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| 1 | Near-infrared photoimmunotherapy of pancreatic cancer using an indocyanine green-labeled anti-tissue factor antibody显示文摘AIM To investigate near-infrared photoimmunotherapeutic effect mediated by an anti-tissue factor(TF) antibody conjugated to indocyanine green(ICG) in a pancreatic cancer model.METHODS Near-infrared photoimmunotherapy(NIR-PIT) is a highly selective tumor treatment that utilizes an antibody-photosensitizer conjugate administration, followed by NIR light exposure. Anti-TF antibody 1849-ICG conjugate was synthesized by labeling of rat IgG2 b anti-TF monoclonal antibody 1849(anti-TF 1849) to a NIR photosensitizer,ICG. The expression levels of TF in two human pancreatic cancer cell lines were examined by western blotting. Specific binding of the 1849-ICG to TF-expressing BxPC-3 cells was examined by fluorescence microscopy. NIR-PITinduced cell death was determined by cell viability imaging assay. In vivo longitudinal fluorescence imaging was used to explore the accumulation of 1849-ICG conjugate in xenograft tumors. To examine the effect of NIRPIT, tumor-bearing mice were separated into 5 groups:(1) 100 μg of 1849-ICG i.v. administration followed by NIR light exposure(50 J/cm2) on two consecutive days(Days 1 and 2);(2) NIR light exposure(50 J/cm2) only on two consecutive days(Days 1 and 2);(3) 100 μg of 1849-ICG i.v. administration;(4) 100 μg of unlabeled antiTF 1849 i.v. administration; and(5) the untreated control. Semiweekly tumor volume measurements, accompanied with histological and immunohistochemical(IHC) analyses of tumors, were performed 3 d after the 2nd irradiation with NIR light to monitor the effect of treatments. RESULTS High TF expression in BxPC-3 cells was observed via western blot analysis, concordant with the observed preferential binding with intracellular localization of 1849-ICG via fluorescence microscopy. NIR-PIT-induced cell death was observed by performing cell viability imaging assay. In contrast to the other test groups, tumor growth was significantly inhibited by NIR-PIT with a statistically significant difference in relative tumor volumes for 27 d after the treatment start date [2.83 ± 0.38(NIR-PIT) vs 5.42 ± 1.61(Untreated), vs 4.90 ± 0.87(NIR), vs 4.28 ±1.87(1849-ICG), vs 4.35 ± 1.42(anti-TF 1849), at Day 27, P < 0.05]. Tumors that received NIR-PIT showed evidence of necrotic cell death-associated features upon hematoxylin-eosin staining accompanied by a decrease in Ki-67-positive cells(a cell proliferation marker) by IHC examination.CONCLUSION The TF-targeted NIR-PIT with the 1849-ICG conjugate can potentially open a new platform for treatment of TF-expressing pancreatic cancer. | Winn Aung Atsushi B Tsuji Aya Sugyo Hiroki Takashima Masahiro Yasunaga Yasuhiro Matsumura Tatsuya Higashi | 2018 | World Journal of Gastroenterology2018，24，48： | 7 |
| 2 | Giant osteoma of the frontal sinus 显示文摘 | Ataman M Ayas K Gursel B | 1993 | Rhinology1993，31，4： | 1 |
| 3 | Healing of periodontal defects treated with enamel matrix proteins and root surface conditioning-an experimental study in dogs显示文摘 | Sakallioglu U Acikgoz G Ayas B | 2004 | Biomaterials2004，25，： | 1 |
| 4 | The effect of abdominal massage on bowel function in patients with spinal cord injury显示文摘 | Ayas S Leblebici B Sozay S | 2006 | Am J Phys Med Rehabil2006，85，12： | 1 |
| 5 | Effects of rise-dronate on alveolar bone loss and angiogenesis: A ste- reologic study in rats显示文摘 | Cetinkaya BO Keles GC Ayas B | 2008 | J Periodontol2008，79，10： | 1 |
| 6 | Healing of periodontal defects treated with enamel matrix proteins and root surface conditioning- an experimental study in dogs显示文摘 | Sakallioglu U Acikgoz G Ayas B | 2004 | Biomaterials2004，25，10： | 1 |
| 7 | Healing of periodontal defects treated with enamel matrix proteins and root surface conditioning-an experimental study in dogs显示文摘 | Sakallioglu U Acikgoz G Ayas B | 2004 | Biomaterials2004，25，10： | 1 |
| 8 | Healing of periodontal defects treated with enamel matrix proteins and root surface conditioning:an experimental study in dogs显示文摘 | Sakallio(g)lu U Asikg(o)z G Ayas B | 2004 | Biomaterials2004，25，10： | 1 |
| 9 | Determination of systemically & locally induced periodontal defects in rats 显示文摘 | Keles GG Acikgoz G Ayas B | 2005 | Indian J Med Res2005，121，3： | 1 |
| 10 | Increased expression of vascular endothelial growth factor in cyclosporin A-induced gingival overgrowth in rats显示文摘 | Cetinkaya BO Acikgoz G Ayas B | 2006 | J Periodontol2006，77，： | 1 |
| 11 | Gingival levels of monocyte chemoattractant protein-1 (MCP-1) in diabetes mellitus and periodontitis: an experimental study in rats显示文摘 | Elif Eser Sakall?o?lu Bülent Ayas Müge Lütfio?lu Gonca ?ayir Kele? G?khan A??kg?z Erhan F?ratl? | 2008 | Clinical Oral Investigations2008，，1： | 1 |
| 12 | Gingival levels of monocyte chemoattractant protein-1 (MCP-1) in diabetes mellitus and periodontitis: an experimental study in rats显示文摘 | Elif Eser Sakall?o?lu Bülent Ayas Müge Lütfio?lu Gonca ?ayir Kele? G?khan A??kg?z Erhan F?ratl? | 2008 | Clinical Oral Investigations2008，，1： | 1 |
| 13 | Serum vitamin D levels are independently associated with severity of coronary artery disease显示文摘 | Akin F Aya B K0se N | 2012 | J Investig Med2012，60，6： | 1 |
| 14 | The effect of abdominal massage on bowel function in patients with spinal cord injury显示文摘 | Ayas S Leblebici B Sozay S | 2006 | American Journal of Physical Medicine & Rehabilitation2006，85，： | 1 |
| 15 | Bves:prototype of a new class of cell adhesion molecules expressed during coronary artery devebpment显示文摘 | Aya M W David E B David M B | 2001 | Development2001，128，： | 1 |
| 16 | The expression of vascular endothelial growth factor in a rat model at destruction and healing stages of periodontal disease显示文摘 | Cetinkaya BO Keles GC Ayas B | 2007 | J Periodontol2007，78，6： | 1 |
| 17 | Gingival levels of monocyte chemoattractant protein-1 ( MCP-1 ) in diabetes mellitus and periodontitis:an xperimental study in rats显示文摘 | Sakalhoglu E E Ayas B Ltitfioglu M | 2008 | Clin Oral Investig2008，12，1： | 1 |
| 18 | Gingival levels of monocyte chemoattractant protein-1 (MCP-1) in diabetes mellitus and periodontitis: an experimental study in rats显示文摘 | Elif Eser Sakall?o?lu Bülent Ayas Müge Lütfio?lu Gonca ?ayir Kele? G?khan A??kg?z Erhan F?ratl? | 2008 | Clinical Oral Investigations2008，，1： | 1 |
| 19 | The effect of abdominal massage on bowel function in patients with spinal cord injury 显示文摘 | Ayas S Leblebici B Sozay S | 2006 | Am J Phys Med Rehabil2006，85，12： | 1 |
| 20 | Strategies for reducing subsurface drainage in irrigated agriculture through improved irrigation显示文摘 | Hanson B R Ayaes J E | 2002 | Irrigation and Drainage Systems2002，16，： | 1 |
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