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| 1 | Hydrogen sutfide protects colon cancer cells from chemopreventative agent β-phenylethyl isothiocvanate induced apoptosis显示文摘AIM: Hydrogen sulfide (H2S) is a prominent gaseous constituent of the gastrointestinal (GI) tract with known cytotoxic properties. Endogenous concentrations of H2S are reported to range between 0.2-3.4 mmol/L in the GI tract of mice and humans. Considering such high levels we speculate that, at non-toxic concentrations, H2S may interact with chemical agents and alter the response of colonic epithelium cells to such compounds. The GI tract is a major site for the absorption of phytochemical constituents such as isothiocyanates, flavonoids, and carotenoids, with each group having a role in the prevention of human diseases such as colon cancer. The chemopreventative properties of the phytochemical agent β-phenyethyl isothiocyanate (PEITC) are well recognized. However, little is currently known about the physiological or biochemical factors present in the GI tract that may influence the biological properties of ITCs. The current study was undertaken to determine the effects of H2S on PEITC mediated apoptosis in colon cancer cells.METHODS: Induction of apoptosis by PEITC in human colon cancer HCT116 cells was assessed using classic apoptotic markers namely SubG1 population analysis,caspase-3 like activity and nuclear fragmentation and condensation coupled with the MTT (3-(4,5-dimethylthiazol2-yl)-2,5-diphenyl tetrasodium bromide) viability assay and LDH leakage.RESULTS: PEITC significantly induced apoptosis in HGT116cells as assessed by SubG1 population formation, nuclear condensation, LDH leakage and caspase-3 activity after 24 h, these data being significant from control groups (P<0.01). In contrast, co-treatment of cells with physiological concentrations of H2S (0.1-1 mmol/L) prevented PEITC mediated apoptosis as assessed using the parameters described.CONCLUSION: PEITC effectively induced cell death in the human adenocarcinoma cell line HCT116 in vitro through classic apoptotic mechanisms. However, in the presence of H2S, apoptosis was abolished. These data suggest that H2S may play a significant role in the response of colonic epithelial cells to beneficial as well as toxic agents present within the GI tract. | Peter Rose Philip K Moore Shen Han Ming Ong Choon Nam Jeffrey S Armstrong Matt Whiteman | 2005 | World Journal of Gastroenterology2005，11，26： | 13 |
| 2 | Phylogenetic relationships among Bactrocera species ( Diptera : Tephritidae) inferred from mitochondrial DNA sequences 显示文摘 | Smith P T Kambhanti S Armstrong K A | 2003 | Mor Phylogenet and Evol2003，26，： | 1 |
| 3 | PCB design techniques for lowestcost EMC compliance:part 1显示文摘 | Armstrong M K | 1999 | Electronics & Communication Engineering Journal1999，11，4： | 1 |
| 4 | Joint MAP registration and high- resolution image estimation using a sequence of under-sampled images 显示文摘 | Hardie R C Barnard K J Armstrong E E | 1997 | IEEETransactions on Image Processing1997，6，12： | 1 |
| 5 | Serial surgical de-bridement: a retrospective study on clinical outcomes in chro-nic lower extremity wounds显示文摘 | Cardinal M Eisenbud DE Armstrong DG Zelen C DriverV Attinqer C Phillips T Harding K | 2009 | Wound Repair Reqen2009，17，3： | 1 |
| 6 | Dietary polyunsaturated fat uniquely suppress rat liver fatty acid synthase and s14m RNA content显示文摘 | Clarke S D Armstrong M K Jump D B | 1990 | Journal of Nutrition1990，120，： | 1 |
| 7 | The effect of treating the virus of tobacco mosaic with the juice of various plants 显示文摘 | Duggar B M Armstrong J K | 1925 | Ann Missouri Bor Garden1925，2，： | 1 |
| 8 | Effect of forced,hot-air treatments of papaya fruit on fruit quality and incidence of postharvest disease显示文摘 | Nishijima K A Miura C K Armstrong J W | 1992 | Plant Dis1992，76，： | 1 |
| 9 | Pathogenic races of the xuxumletwilt fusarium显示文摘 | Armsrong G M Armstrong J K Neter D | 1978 | Plant Dis Report1978，，62： | 1 |
| 10 | PCB design techniques for lowestcost EMC compliance:part 2显示文摘 | Armstrong M K | 1999 | Electronics & Communication Engineering Journal1999，11，5： | 1 |
| 11 | DNA barcodes for biosecurity: invasive species identification显示文摘 | Armstrong K F Ball S L | 2005 | Phil Trans R Soc B2005，360，： | 1 |
| 12 | Canadian cons- ensus conference on the management of gastroesophageal reflux disease in adults-update 2004显示文摘 | Armstrong D Marshall J K Chiba N | 2005 | Can J Gastroen- terol2005，19，1： | 1 |
| 13 | X-ray reflection topograph- ic study of growth defect and microindentation strain fields in an RDX explosive crystal 显示文摘 | Elban W Armstrong R Yoo K | 1989 | Journal of Materials Science1989，24，4： | 1 |
| 14 | Compar- ative evaluation of the visibility and block characteristics of a stimulating needle and catheter vs an echogenic nee- dle and catheter for sciatic nerve block with a low-fre- quency ultrasound probe显示文摘 | Brookes J Sondekoppam R Armstrong K | 2015 | Br J Anaesth2015，115，6： | 1 |
| 15 | Phylogenetic relationships among Bactrocera species (Diptera: Tephritidae) inferred from mitochondrial DNA sequences显示文摘 | Smith P T Kambhampati S Armstrong K A | 2003 | Mol Phylo genet Evol2003，26，1： | 1 |
| 16 | Invasive phy- tophagous pests arising through a recent evolutionary radiation: theBaetrocera dorsalis complex of tropical fruit flies显示文摘 | CLARKE A R ARMSTRONG K F CARMICHAEL A E MILNE J R RAGHU S RODERICK G K YEATES D K | 2005 | Annual Review of Entomology2005，50，： | 1 |
| 17 | A New class of Chiral selector for Hight Performance Liquid Chromatogr 显示文摘 | Armstrong D W Liu Y B Erhoryott K H | 1995 | Chirality1995，，7： | 1 |
| 18 | Seed and seedling biology in relation to modeling vegetation dynamics under global climate change显示文摘 | Leishman M R Hughes L French K Armstrong D Westoby M | 1992 | Aust J Bot1992，40，： | 1 |
| 19 | Dietary polyunsaturated fats uniquely suppress rat liver fatty acid synthase and S14 mRNA conten显示文摘 | Clarke S D Armstrong M K Jump D B | 1990 | J Nutr1990，120，： | 1 |
| 20 | Transformation of rainfall by plant canopy显示文摘 | Armstrong C L Mitchell J K | 1987 | Transactions of the ASAE1987，30，3： | 1 |
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