|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Detection of tumor stem cell markers in pancreatic carcinoma cell lines显示文摘BACKGROUND: Cancer of the pancreas is the fourth leading cause of cancer death in industrialized countries. In malignancy, actively proliferating cells may be effectively targeted and killed by anti-cancer therapies, but stem cells may survive and support re-growth of the tumor. Thus, new strategies for the treatment of cancer clearly will also have to target cancer stem cells. The goal of the present study was to determine whether pancreatic carcinoma cell growth may be driven by a subpopulation of cancer stem cells. Because previous data implicated ABCG2 and CD133 as stem cell markers in hematopoietic and neural stem/progenitor cells, we analyzed the expression of these two proteins in pancreatic carcinoma cell lines. METHODS: Five established pancreatic adenocarcinoma cell lines were analyzed. Total RNA was isolated and real- time RT-PCR was performed to determine the expression of ABCG2 and CD133. Surface expression of ABCG2 and CD133 was analyzed by flow cytometric analysis. RESULTS: All pancreatic carcinoma cell lines tested expressed significantly higher levels of ABCG2 than non-malignant fibroblasts or two other malignant non- pancreatic cell lines, i.e., SaOS2 osteosarcoma and SKOV3 ovarian cancer. Elevated CD133 expression was found in two out of five pancreatic carcinoma cell lines tested. Using flow cytometric analysis we confirmed surface expression of ABCG2 in all five lines. Yet, CD133 surface expression was detectable in the two cell lines, A818-6 and PancTu1, which exhibited higher mRNA levels.CONCLUSIONS: Two stem cell markers, ABCG2 and CD133 are expressed in pancreatic carcinoma cell lines. ABCG2 and/or CD133 positive cells may represent subpopulation of putative cancer stem cells also in this malignancy. Because cancer stem cells are thought to be responsible for tumor initiation and its recurrence after an initial response to chemotherapy, they may be a very promising target for new drug developments. | Monika Olempska Patricia Alice Eisenach Ole Ammerpohl Hendrik Ungefroren Fred Fandrich Holger Kalthoff | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，1： | 69 |
| 2 | Activation of Wnt signalling in stroma from pancreatic cancer identified by gene expression profiling 显示文摘 | Pilarsky C Ammerpohl O Sipos B | 2008 | J Cell Mol Med2008，12，6： | 1 |
| 3 | Gap junction-m- ediated bystander effect in primary cultures of human malignant gliomas with recombinant expression of the HSVtk gene显示文摘 | Asklund T Appelskog IB Ammerpohl O | 2003 | Exp Cell Res2003，284，2： | 1 |
| 4 | Hunting for the 5th base: techniques for analyzing DNA methylation 显示文摘 | Ole Ammerpohl Jose I Martin-Subero | 2009 | Biochim Biophys Acta2009，1790，： | 1 |
| 5 | Expression of L amino acid transport system 1 and analysis of iodine-123-methyltyrosine tumor uptake in a pancreatic xenotransplantation model using fused high-resolution-micro-SPECT-MRI显示文摘BACKGROUND:The specificity in discriminating pancreatitis is limited in the positron emission tomography(PET)using Fluorine-18-fluorodeoxyglucose.Furthermore,PET is not widely available compared to the single photon emission computed tomography(SPECT).Since amino acids play a minor role in metabolism of inflammatory cells,the potential of the SPECT tracer,3-[ 123 I]iodo-L-α-methyltyrosine(123I-IMT),for detecting pancreatic cancer was examined in xenotransplantation models of human pancreatic carcinoma in mice. METHODS: 123 I-IMT was injected to eight mice inoculated with subcutaneous or orthotopic pancreatic tumors.Fused high-resolution-micro-SPECT(Hi-SPECT)and magnetic resonance imaging were performed.The gene expression level of L amino acid transport-system 1(LAT1)was analyzed and correlated with tumor uptake of 123 I-IMT. RESULTS:A high uptake of 123 I-IMT was detected in all tumor-bearing mice.The median tumor-to-background ratio (T/B)was 12.1(2.0-13.2)for orthotopic and 8.4(1.8-11.1)for subcutaneous xenotransplantation,respectively.Accordingly, the LAT1 expression in transplanted Colo357 cells was increased compared to non-malignant controls.CONCLUSIONS:Our mouse model could show a high 123 I-IMT uptake in pancreatic cancer.Fused MRI scans facilitate precise evaluation of uptake in the specific regions of interest.Further studies are required to confirm these findings in tumors derived from other human pancreatic cancer cells.Since amino acids play a minor role in the metabolism of inflammatory cells,the potential for application of 123 I-IMT to distinguish pancreatic tumor from inflammatory pancreatitis warrants further investigation. | Corinna von Forstner Maaz Zuhayra Ole Ammerpohl Sanjay Tiwari Olav Jansen Holger Kalthoff Eberhard Henze Jan-Hendrik Egberts | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，1： | 1 |
| 6 | Detection of tumor stem cell markers in pancreatic carcinoma cell lines显示文摘 | Olempska M Eisenach P A Ammerpohl O Ungefroren H Fandrich F Kalthoff H | 2007 | Hepatobiliary Pancreat Dis Int2007，6，： | 1 |
| 7 | Activation of Wnt sig- nalling in stroma from pancreatic cancer identified by gene expres- sion profiling显示文摘 | Pilarsky C Ammerpohl O Sipos B | 2008 | J Cell Mol Med2008，12，6： | 1 |
| 8 | HDACi phenylbutyrate increases bystander killing of HSV-tk transfected glioma cells显示文摘 | Ammerpohl O Thormeyerb D Khana Z | 2004 | Biochemical and Biophysical Research Communications2004，324，1： | 1 |
| 9 | HDACi phenylbutyrate increases bystander killing of HSV-tk transfected glioma cells显示文摘 | AMMERPOHL O THORMEYER D KHAN Z | 2004 | Biochem Biophys Res Commun2004，324，1： | 1 |
| 10 | Gap junction-mediated bystander effect in primary cultures of human malignant gliomas with recombinant expression of the HSVtk gene显示文摘 | Asklund T Appelskog IB Ammerpohl O | 2003 | Exp Cell Res2003，284，2： | 1 |
| 11 | Gap junction-mediated bystander effect in primary cultures of human malignant gliomas with recombinant expression of the HSVtk gene显示文摘 | Asklund T Appelskog IB Ammerpohl O | 2003 | Exp Cell Res2003，284，2： | 1 |
| 12 | HDACi phenylbutyrate increases bystander killing of HSV-tk transfected glioma cells显示文摘 | Ammerpohl O Thormeyer D Khan Z | 2004 | Biochem Biophys Res Commun2004，324，1： | 1 |
| 13 | Array-based DNA methylation profiling of primary lymphomas of the central nervous system显示文摘 | Richter J Ammerpohl O Martin-Subero JI | 2009 | BMC cancer2009，9，： | 1 |
| 14 | Gap junction mediated bystander effect in primary cultures of human malignant gliomas with recombinant expression of the HSVtk geng显示文摘 | Asklund T Appelskog IB Ammerpohl 0 | 2003 | Exp Cell Res2003，284，2： | 1 |
| 15 | Progression-free survival in ovarian cancer is reflected in epigenetic DNA methylation profiles 显示文摘 | Bauerschlag DO Ammerpohl O Brautigam K | 2011 | Oncology2011，80，112： | 1 |
| 16 | Meta-analysis of microarray data on pancreatic cancer defines a set of commonly dysregulated genes显示文摘 | GRUTZMANN R BORISS H AMMERPOHL 0 etal | 2005 | Oncogene2005，24，32： | 1 |
| 17 | Gene expression profiling of microdissected pancreatic ductal carcinomas using high-density DNA microarrays显示文摘 | GRUTZMANN R PILARSKY C AMMERPOHL O | 2004 | Neoplasia2004，6，： | 1 |
| 18 | Gap junction-mediated bystander effect in primary cultures of human malignant gliomas with recombinant expression of the HSV-TK gene显示文摘 | Asklund T Appelskog IB Ammerpohl O | 2003 | Exp Cell Res2003，284，2： | 1 |
| 19 | Gap junction-mediated bystander effect in primary cultures of human malignant gliomas with recombinant expression of the HSV-TK gene显示文摘 | Asklund T Appelskog I B Ammerpohl O | 2003 | Exp Cell Res2003，284，2： | 1 |
| 20 | Array-based DNA methylation profiling of primary lymphomas of the central nervous system显示文摘 | Richter J Ammerpohl O Martin-Subero JI | 2009 | BMCCancer2009，9，21： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员