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| 1 | Role of hydroxypropyl-β-cyclodextrin on freeze-dried and gamma-irradiated PLGA and PLGA–PEG diblock copolymer nanospheres for ophthalmic flurbiprofen delivery显示文摘 | Estefanía Vega García Espina Calpena Egea | 2012 | International Journal of Nanomedicine (default)2012，，： | 1 |
| 2 | Solidarity in interactive groups in a Learning Community of Spain显示文摘The Learning Communities are an educational project.Its aim is to achieve quality education for children,youth and adults.The method to complete this goal is educational activities as interactive groups.In this paper,we present the solidarity of the students in interactive groups.For this reason,we use the critical communicative method.The research techniques are communicative observation and document analysis.The data has been obtained a learning community in Spain.The results of the analysis show that in interactive groups,the students help each other.Likewise,they cheer up and wait for each other. | Estefanía Fernández Nimbe Arellanos | 2018 | Journal of Contemporary Educational Research2018，2，3： | 0 |
| 3 | Effect of iron and manganese on hydroxyl radical production by 6-hydroxydopamine:mediation of antioxidants显示文摘 | Estefanía M Ramón SO lvaro HA | | 0，，08： | 1 |
| 4 | DNA replication fading as proliferating cells advance in their co-mmitment to terminal differentiation显示文摘 | Estefanía MM Ganier O Hernández P | | 0，，： | 1 |
| 5 | Gene regulatory networks in atrial fibrillation显示文摘Atrial fibrillation(AF)is the most frequent arrhythmogenic syndrome in humans.With an estimate incidence of1%-2%in the general population,AF raises up to almost10%-12%in 80+years.Thus,AF represents nowadays a highly prevalent medical problem generating a large economic burden.At the electrophysiological level,distinct mechanisms have been elucidated.Yet,despite its prevalence,the genetic and molecular culprits of this pandemic cardiac electrophysiological abnormality have remained largely obscure.Molecular genetics of AF familiar cases have demonstrated that single nucleotide mutations in distinct genes encoding for ion channels underlie the onset of AF,albeit such alterations only explain a minor subset of patients with AF.In recent years,analyses by means of genome-wide association studies have unraveled a more complex picture of the etiology of AF,pointing out to distinct cardiac-enriched transcription factors,as well as to other regulatory genes.Furthermore a new layer of regulatory mechanisms have emerged,i.e.,post-transcriptional regulation mediated by non-coding RNA,which have been demonstrated to exert pivotal roles in cardiac electrophysiology.In this manuscript,we aim to provide a comprehensive review of the genetic regulatory networks that if impaired exert electrophysiological abnormalities that contribute to the onset,and subsequently,on self-perpetuation of AF. | Diego Franco Estefanía Lozano-Velasco Amelia Aranega | 2016 | World Journal of Medical Genetics2016，6，1： | 0 |
| 6 | Bioanalytical separation and preconcentration using ionic liquids显示文摘 | Leticia B. Escudero Alexander Castro Grijalba Estefanía M. Martinis Rodolfo G. Wuilloud | 2013 | Analytical and Bioanalytical Chemistry2013，，24： | 1 |
| 7 | Flurbiprofen PLGA-PEG nanospheres: Role of hydroxy-β-cyclodextrin on ex vivo human skin permeation and in vivo topical anti-inflammatory efficacy显示文摘 | Estefanía Vega M. Antònia Egea M. Luisa Gardu?o-Ramírez M. Luisa García Elena Sánchez Marta Espina Ana Cristina Calpena | 2013 | Colloids and Surfaces B: Biointerfaces2013，，： | 1 |
| 8 | In vitro and in vivo evaluation of lactic acid bacteria of aquatic origin as probiotics for turbot (Scophthalmus maximus ) farming 显示文摘 | EstefaníM-A Carlos A Susana M Pablo EH Carmen H Ysabel S Luis MC | 2014 | Fish Shellfish Immun2014，41，2： | 1 |
| 9 | AC and DC technology in microgrids: A review显示文摘 | Estefanía Planas Jon Andreu José Ignacio Gárate I?igo Martínez de Alegría Edorta Ibarra | 2014 | Renewable and Sustainable Energy Reviews2014，，： | 1 |
| 10 | Temporal development of genetic and metabolic effects of biotin deprivation. A search for the optimum time to study a vitamin deficiency显示文摘 | Alain Hernández-Vázquez Estefanía Ochoa-Ruiz Isabel Ibarra-González Daniel Ortega-Cuellar Ana Salvador-Adriano Antonio Velázquez-Arellano | 2012 | Molecular Genetics and Metabolism2012，，3： | 1 |
| 11 | New frontiers in focal therapy for prostate cancer:Prostate-specific membrane antigen positron emission tomography/magnetic resonance imaging显示文摘Imaging has a central role in the context of focal therapy(FT)for prostate cancer(PCa).Prostate-specific membrane antigen(PSMA)positron emission tomography/magnetic resonance imaging(PET/MRI)is a novel imaging modality that combines the morpho-functional information of MRI with the molecular characterization of PET.Some papers reported the potential advantages of PSMA PET/MRI in different clinical scenarios.Limited evidence on PSMA PET/MRI is available in the setting of FT.PSMA PET/MRI can be an effective imaging modality for detecting primary PCa and seems to provide accurate local staging of primary PCa.PSMA PET/MRI also shows high performance for restaging and detecting tumor recurrence.The higher soft-tissue contrast and the reduction of ionizing radiation are the main advantages reported in the literature compared to PET/computed tomography.PSMA PET/MRI could represent a turning point in the management of patients with PCa in the context of FT.Further studies are needed to confirm its applications in this specific clinical setting. | Celeste Manfredi Esau Fernandez-Pascual Estefanía Linares-Espinós Felipe Couñago Juan Ignacio Martínez-Salamanca | 2021 | World Journal of Clinical Oncology2021，12，2： | 0 |
| 12 | 感染非洲猪瘟强毒株和弱毒株的野猪排毒方式的差异显示文摘自从非洲猪瘟病毒(ASFV)再次出现以来,该病在欧亚大陆以前所未有的动物大流行方式传播。目前,ASF是全球养猪业面临的最大问题。野猪是ASFV的关键宿主,病毒可以在其中独立循环传播,因此迫切需要研制一种有效的疫苗来抵抗该病毒。目前的科学争论在于:出于安全考虑,接种已经表现出交叉保护易感宿主方面良好效果的减毒活疫苗(LAVs)是否可行。因此,研究的目的是比较ASFV强毒株和弱毒株(LAV)感染野猪后的排毒情况。接种Lv17/WB/Rie1分离株和Armenia07强毒株的野猪表现出了不同的病毒排出途径(唾液和粪便)和病毒血症率。与感染Armenia07强毒株的动物相比,感染Lv17/WB/Rie1分离株动物的血液、唾液、粪便中较少检测出阳性。通过描述排毒特征来理解其传播动力学。这部分知识将有助于评估野猪中新弱毒株的排毒模式,其中还包括与目前最有应用前景的基因缺失突变减毒活疫苗进行比较。 | 张文志(编译) 刘金(编译) Aleksandra Kosowska Estefanía Cadenas-Fernández Sandra Barroso | 2021 | 猪业科学2021，38，8： | 0 |
| 13 | Evolution of wheat architecture,physiology,and metabolism during domestication and further cultivation:Lessons for crop improvement显示文摘In recent decades,genetic advances in yield improvement in the major cereal crops,including wheat,has stagnated or proceeded at a slower rate than is required to meet future global food demand,particularly in the face of climate change.To reverse this situation,and in view of the future climate scenario,there is a need to increase the genetic diversity of wheat to increase its productivity,quality,stability,and adaptation to local agro-environments.The abundant genetic resources and literature are a basis for wheat improvement.However,many species,such as wild relatives,landraces,and old cultivars have not been studied beyond their agronomic characteristics,highlighting the lack of understanding of the physiological and metabolic processes(and their integration) associated with higher productivity and resilience in limiting environments.Retrospective studies using wheat ancestors and modern cultivars may identify novel traits that have not previously been considered,or have been underestimated,during domestication and breeding,but that may contribute to future food security.This review describes existing wheat genetic diversity and changes that occurred during domestication and breeding,and considers whether mining natural variation among wheat ancestors offers an opportunity to enhance wheat agronomic performance,spike architecture,canopy-and organ-level photosynthetic capacity,and responses to abiotic stress,as well as to develop new wheat hybrids. | Arturs Katamadze Omar Vergara-Díaz Estefanía Uberegui Ander Yoldi-Achalandabaso José Luis Araus Rubén Vicente | 2023 | The Crop Journal2023，11，4： | 0 |
| 14 | Healthcare staff as promoters of parental presence at anesthetic induction:Net Promoter Score survey显示文摘BACKGROUND Surgical intervention is usually a traumatic event that causes stress and anxiety in the pediatric patient and the family environment.To reduce the harmful effects of presurgical anxiety,parental presence during induction of anesthesia(PPIA)is one of the more notable interventions used in medical centers.However,data on this measure are difficult to evaluate and often face resistance from healthcare staff.AIM To analyze the perception of the healthcare workers after the implementation of a PPIA program.METHODS A survey was developed and sent by email to all the healthcare staff working in the children’s area of a tertiary hospital.It consisted of 14 items divided into positive aspects of PPIA and negative aspects of PPIA evaluated with the use of a Likert scale(1 to 5).The demographics of the respondents were included in the data collected.The answers to the questions were interpreted through the Net Promoter Score(NPS).The statistical analysis compared the differences in the responses to each question of the survey made by the different groups of health personnel included.RESULTS A total of 141 surveys were sent out,with a response rate of 69%.Of the total number of responses,68%were from women and 32%from men.The average age of the participants was 42.3±10.6 years.As for the positive questions about the PPIA,83%had an NPS>50,and only one had a score between 0 and 50,which means that the quality of the service was rated as excellent or good by 100%of the respondents.On the other hand,100%of the negative questions about the PPIA had a negative NPS.Responses to the question“PPIA increases patient safety”were significantly different(P=0.037),with a lower percentage of pediatric surgeons(70%)thinking that PPIA increased patient safety,compared with anesthesiologists(90%),nursing(92%),and other medical personnel(96%).CONCLUSION The personnel who participated in the PPIA program at our center were in favor of implementation.There were no validated arguments to support worker resistance to the development of the PPIA. | María Velayos Karla Estefanía María Alvarez María C Sarmiento Lucas Moratilla Pascual Sanabria Francisco Hernández Manuel V López Santamaría | 2021 | World Journal of Clinical Pediatrics2021，10，6： | 1 |
| 15 | In vitro and in vivo evaluation of lactic acid bacteria of aquatic origin as probiotics for turbot ( Scophthalmus maximus L.) farming显示文摘 | Estefanía Mu?oz-Atienza Carlos Araújo Susana Magadán Pablo E. Hernández Carmen Herranz Ysabel Santos Luis M. Cintas | 2014 | Fish and Shellfish Immunology2014，，： | 1 |
| 16 | 墨西哥湖屋显示文摘Casa del Lago是一栋毗邻水域的独立住宅。住户为一对拥有多元文化的成熟夫妇。设计师利用建筑场域的特点,将开放的公共空间也规划成为日常使用动线的一部分,并将墨西哥尤卡坦文化融入空间之中,同时让房屋还可以容纳主人的艺术收藏品。 | Carlos Patrón Carlos Patrón lbarra Alejandro Patrón Sansor Ana Pat ró n Ibarra Estefanía Rivero Janssen Joaquín Mu?oz Olivera Leo Espinosa 李薇 | 2019 | 现代装饰2019，0，11： | 0 |
| 17 | Mesenchymal stem cell-derived exosomes promote neurogenesis and cognitive function recovery in a mouse model of Alzheimer’s disease显示文摘Studies have shown that mesenchymal stem cell-derived exosomes can enhance neural plasticity and improve cognitive impairment.The purpose of this study was to investigate the effects of mesenchymal stem cell-derived exosomes on neurogenesis and cognitive capacity in a mouse model of Alzheimer’s disease.Alzheimer’s disease mouse models were established by injection of beta amyloid 1?42 aggregates into dentate gyrus bilaterally.Morris water maze and novel object recognition tests were performed to evaluate mouse cognitive deficits at 14 and 28 days after administration.Afterwards,neurogenesis in the subventricular zone was determined by immunofluorescence using doublecortin and PSA-NCAM antibodies.Results showed that mesenchymal stem cells-derived exosomes stimulated neurogenesis in the subventricular zone and alleviated beta amyloid 1?42-induced cognitive impairment,and these effects are similar to those shown in the mesenchymal stem cells.These findings provide evidence to validate the possibility of developing cell-free therapeutic strategies for Alzheimer’s disease.All procedures and experiments were approved by Institutional Animal Care and Use Committee(CICUAL)(approval No.CICUAL 2016-011)on April 25,2016. | Edwin E. Reza-Zaldivar Mercedes A. Hernández-Sapiéns Yanet K. Gutiérrez-Mercado Sergio Sandoval-ávila Ulises Gomez-Pinedo Ana L. Márquez-Aguirre Estefanía Vázquez-Méndez Eduardo Padilla-Camberos Alejandro A. Canales-Aguirre | 2019 | Neural Regeneration Research2019，14，9： | 19 |
| 18 | Use of a combination strategy to improve neuroprotection and neuroregeneration in a rat model of acute spinal cord injury显示文摘Spinal cord injury is a very common pathological event that has devastating functional consequences in patients. In recent years, several research groups are trying to find an effective therapy that could be applied in clinical practice. In this study, we analyzed the combination of different strategies as a potential therapy for spinal cord injury. Immunization with neural derived peptides(INDP), inhibition of glial scar formation(dipyridyl: DPY), as well as the use of biocompatible matrix(fibrin glue: FG) impregnated with bone marrow mesenchymal stem cells(MSCs) were combined and then its beneficial effects were evaluated in the induction of neuroprotection and neuroregeneration after acute SCI. Sprague-Dawley female rats were subjected to a moderate spinal cord injury and then randomly allocated into five groups: 1) phosphate buffered saline; 2) DPY; 3) INDP + DPY; 4) DPY+ FG; 5) INDP + DPY + FG + MSCs. In all rats, intervention was performed 72 hours after spinal cord injury. Locomotor and sensibility recovery was assessed in all rats. At 60 days after treatment, histological examinations of the spinal cord(hematoxylin-eosin and Bielschowsky staining) were performed. Our results showed that the combination therapy(DPY+ INDP + FG+ MSCs) was the best strategy to promote motor and sensibility recovery. In addition, significant increases in tissue preservation and axonal density were observed in the combination therapy group. Findings from this study suggest that the combination theapy(DPY+ INDP + FG + MSCs) exhibits potential effects on the protection and regeneration of neural tissue after acute spinal cord injury. All procedures were approved by the Animal Bioethics and Welfare Committee(approval No. 178544; CSNBTBIBAJ 090812960)on August 15, 2016. | Elisa García Roxana Rodríguez-Barrera Vinnitsa Buzoianu-Anguiano Adrian Flores-Romero Emanuel Malagón-Axotla Marco Guerrero-Godinez Estefanía De la Cruz-Castillo Laura Castillo-Carvajal Monserrat Rivas-Gonzalez Paola Santiago-Tovar Ivis Morales Cesar Borlongan Antonio Ibarra | 2019 | Neural Regeneration Research2019，14，6： | 6 |
| 19 | Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort显示文摘BACKGROUND Colorectal cancer(CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2(COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and stability. Since metabolic and proteomic profile analysis is essential for cancer prognosis and diagnosis, our hypothesis is that the analysis of correlations between these specific parameters and COX-2 state in tumors of a high number of CRC patients could be useful for the understanding of the basis of this cancer in humans.AIM To analyze COX-2 regulation in colorectal cancer and to perform a detailed analysis of their metabolic and proteomic profile.METHODS Biopsies from both healthy and pathological colorectal tissues were taken under informed consent from patients during standard colonoscopy procedure in the University Hospital of Bellvitge(Barcelona, Spain) and Germans Trias i Pujol University Hospital(Campus Can Ruti)(Barcelona, Spain). Western blot analysis was used to determine COX-2 levels. Deglycosylation assays were performed in both cells and tumor samples incubating each sample with peptide N-glycosidase F(PNGase F). Prostaglandin E2(PGE2) levels were determined using a specific ELISA. 1 H high resolution magic angle spinning(HRMAS) analysis was performed using a Bruker AVIII 500 MHz spectrometer and proteomic analysis was performed in a nano-liquid chromatography-tandem mass spectrometer(nano LC-MS/MS) using a QExactive HF orbitrap MS.RESULTS Our data show that COX-2 has a differential expression profile in tumor tissue of CRC patients vs the adjacent non-tumor area, which correspond to a glycosylated and less active state of the protein. This fact was associated to a lesser PGE2 production in tumors. These results were corroborated in vitro performing deglycosylation assays in HT29 cell line where COX-2 protein profile was modified after PNGase F incubation, showing higher PGE2 levels. Moreover,HRMAS analysis indicated that tumor tissue has altered metabolic features vs non-tumor counterparts, presenting increased levels of certain metabolites such as taurine and phosphocholine and lower levels of lactate. In proteomic experiments, we detected an enlarged number of proteins in tumors that are mainly implicated in basic biological functions like mitochondrial activity,DNA/RNA processing, vesicular trafficking, metabolism, cytoskeleton and splicing.CONCLUSION In our colorectal cancer cohort, tumor tissue presents a differential COX-2 expression pattern with lower enzymatic activity that can be related to an altered metabolic and proteomic profile. | Patricia Prieto Rafael I Jaén Daniel Calle María Gómez-Serrano Estefanía Nú?ez María Fernández-Velasco Paloma Martín-Sanz Sergio Alonso Jesús Vázquez Sebastián Cerdán Miguel ángel Peinado Lisardo Boscá | 2019 | World Journal of Gastroenterology2019，25，4： | 4 |
| 20 | Exploiting the diversity of tomato:the development of a phenotypically and genetically detailed germplasm collection显示文摘A collection of 163 accessions,including Solanum pimpinellifolium,Solanum lycopersicum var.cerasiforme and Solanum lycopersicum var.lycopersicum,was selected to represent the genetic and morphological variability of tomato at its centers of origin and domestication:Andean regions of Peru and Ecuador and Mesoamerica.The collection is enriched with S.lycopersicum var.cerasiforme from the Amazonian region that has not been analyzed previously nor used extensively.The collection has been morphologically characterized showing diversity for fruit,flower and vegetative traits.Their genomes were sequenced in the Varitome project and are publicly available(solgenomics.net/projects/varitome).The identified SNPs have been annotated with respect to their impact and a total number of 37,974 out of 19,364,146 SNPs have been described as high impact by the SnpEeff analysis.GWAS has shown associations for different traits,demonstrating the potential of this collection for this kind of analysis.We have not only identified known QTLs and genes,but also new regions associated with traits such as fruit color,number of flowers per inflorescence or inflorescence architecture.To speed up and facilitate the use of this information,F2 populations were constructed by crossing the whole collection with three different parents.This F2 collection is useful for testing SNPs identified by GWAs,selection sweeps or any other candidate gene.All data is available on Solanaceae Genomics Network and the accession and F2 seeds are freely available at COMAV and at TGRC genebanks.All these resources together make this collection a good candidate for genetic studies. | Estefanía Mata-Nicolás Javier Montero-Pau Esther Gimeno-Paez Víctor Garcia-Carpintero Peio Ziarsolo Naama Menda Lukas A.Mueller JoséBlanca Joaquín Cañizares Esther van der Knaap María JoséDíez | 2020 | Horticulture Research2020，7，1： | 2 |
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