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| 1 | Preventative effects of a probiotic, Lactobacillus salivarius ssp. salivarius, in the TNBS model of rat colitis显示文摘AIM: To investigate the intestinal anti-inflammatory effect and mechanism of a probiotic Lactobacillus sakuvarius ssp.salivariusCECT5713 in the TNBS model of rat colitis.METHODS: Female Wistar rats (180-200 g) were used in this study. A group of rats were administered orally the probiotic L. salivarius ssp. salivarius (5×108 CFU suspended in 0.5 mL of skimmed milk) daily for 3 wk. Two additional groups were used for reference, a non-colitic and a control colitic without probiotic treatment, which received orally the vehicle used to administer the probiotic. Two weeks after starting the experiment, the rats were rendered colitic by intracolonic administration of 10 mg of TNBS dissolved in 0.25 mL of 500 mL/L ethanol. One week after colitis induction, all animals were killed and colonic damage was evaluated both histologically and biochemically. The biochemical studies performed in colonic homogenates include determination of myeloperoxidase (MPO) activity,glutathione (GSH) content, leukotriene B4 (LTB4) and tumor necrosis factor α (TNF-α) levels, as well as inducible nitric oxide synthase (iNOS) expression. In addition, the luminal contents obtained from colonic samples were used for microbiological studies, in order to determine Lactobacilli and Bifidobacteria counts.RESULTS: Treatment of colitic rats with L. salivarius ssp.salivarius resulted in amelioration of the inflammatory response in colitic rats, when compared with the corresponding control group without probiotic treatment. This antiinflammatory effect was evidenced macroscopically by a significant reduction in the extent of colonic necrosis and/or inflammation induced by the administration of TNBS/ethanol (2.3+0.4 cm vs3.4+0.3 cm in control group,P<0.01) and histologically by improvement of the colonic architecture associated with a reduction in the neutrophil infiltrate in comparison with non-treated colitic rats. The latter was confirmed biochemically by a significant reduction of colonic MPO activity (105.3+26.0 U/g vs180.6±21.9 U/g, P<0.05), a marker of neutrophil infiltration. The beneficial effect was associated with an increase of the colonic GSH content (1 252±42 nmol/g vs 1 087+51 nmol/g, P<0.05),which is depleted in colitic rats, as a consequence of the oxidative stress induced by the inflammatory process. In addition, the treatment of colitic rats with L. salivarius resulted in a significant reduction of colonic TNF-α levels (509.4±68.2 pg/g vs782.9±60.1 pglg, P<0.01) and in a lower colonic iNOS expression, when compared to TNBS control animals without probiotic administration. Finally,treated colitic rats showed higher counts of Lactobacilli species in colonic contents than control colitic rats, whereas no differences were observed in Bifidobacteria counts.CONCLUSION: Administration of the probiotic L. salivarius ssp. salivarius CECT5713 facilitates the recovery of the inflamed tissue in the TNBS model of rat colitis, an effect associated with amelioration of the production of some of the mediators involved in the inflammatory response in the intestine, such as cytokines, including TNF-α and NO. This beneficial effect could be ascribed to its effect on the altered immune response that occurs in this inflammatory condition. | Laura Peran Desiree Camuesco Monica Comalada Ana Nieto Angel Concha Maria Paz Diaz-Ropero Monica Olivares Jordi Xaus Antonio Zarzuelo Julio Galvez | 2005 | World Journal of Gastroenterology2005，11，33： | 17 |
| 2 | L-肉碱对仔猪的促生长作用显示文摘L┐肉碱对仔猪的促生长作用JFGalvez等著李长忠摘译郭年藩校肉碱(carnitine)也叫维生素Bt,仔猪日粮中添加L-肉碱能改善能量代谢过程和生长性能,且能节省赖氨酸和蛋氨酸的用量。在能量代谢过程中,L-肉碱以脂酰和乙酰肉碱的形式结合被活化的脂... | JF Galvez 李长忠 郭年藩 | 1997 | 国外畜牧科技1997，24，2： | 10 |
| 3 | Clinicopathological predictors of long-term benefit in breast cancer treated with neoadjuvant chemotherapy显示文摘AIM To investigate the survival impact of clinicopathological factors, including pathological complete response(p CR) and tumor-infiltrating lymphocytes(s TIL) levels according to subtypes, in breast cancer(BC) patients who received neo-adjuvant chemotherapy(NAC).METHODS We evaluated 435 BC patients who presented and received NAC at the Instituto Nacional de Enfermedades Neoplasicas from 2003 to 2014. s TIL was analyzed as the proportion of tumor stroma occupied by lymphocytes, and was prospectively evaluated on hematoxylin and eosin-stained sections of the preN AC core biopsy. p CR was considered in the absence of infiltrating cancer cells in primary tumor and axillary lymph nodes. Analysis of statistical association between clinical pathological features, s TIL, p CR and survival were carried out using SPSSvs19.RESULTS Median age was 49 years(range 24-84 years) and the most frequent clinical stage was ⅢB(58.3%). Luminal A, Luminal B, HER2-enriched and(triple-negative) TN phenotype was found in 24.6%, 37.9%, 17.7% and 19.8%, respectively. p CR was observed in 11% and median percentage of s TIL was 40%(2%-95%) in the whole population. p CR was associated to Ct1-2(P = 0.045) and to high s TIL(P = 0.029) in the whole population. There was a slight trend towards significance for s TIL(P = 0.054) in Luminal A. s TIL was associated with grade Ⅲ(P < 0.001), no-Luminal A subtype(P < 0.001), RE-negative(P < 0.001), PgR-negative(P < 0.001), HER2-positive(P = 0.002) and p CR(P = 0.029) in the whole population. Longer disease-free survival was associated with grade Ⅰ-Ⅱ(P = 0.006), cN 0(P < 0.001), clinical stage Ⅱ(P = 0.004), ER-positive(P < 0.001), Pg R-positive(P < 0.001), luminal A(P < 0.001) and p CR(P = 0.002). Longer disease-free survival was associated with grade Ⅰ-Ⅱ in Luminal A(P < 0.001), N0-1 in Luminal A(P = 0.045) and TNBC(P = 0.01), clinical stage Ⅱ in Luminal A(P = 0.003) and TNBC(P = 0.038), and pC R in TNBC(P < 0.001). Longer overall survival was associated with grade Ⅰ-Ⅱ(P < 0.001), ER-positive(P < 0.001), PgR-positive(P < 0.001), Luminal A(P < 0.001), cN 0(P = 0.002) and p CR(P = 0.002) in the whole population. Overall survival was associated with clinical stage Ⅱ(P = 0.017) in Luminal A, older age(P = 0.042) in Luminal B, and pC R in TNBC(P = 0.005).CONCLUSION Predictive and prognostic values of clinicopathological features, like p CR and s TIL, differ depending on the evaluated molecular subtype. | Marco Galvez Carlos A Castaneda Joselyn Sanchez Miluska Castillo Lia Pamela Rebaza Gabriela Calderon Miguel De La Cruz Jose Manuel Cotrina Julio Abugattas Jorge Dunstan Henry Guerra Omar Mejia Henry L Gomez | 2018 | World Journal of Clinical Oncology2018，9，2： | 4 |
| 4 | Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin America显示文摘 | Lena Galvez Ranilla Young-In Kwon Emmanouil Apostolidis Kalidas Shetty | 2010 | Bioresource Technology2010，，12： | 4 |
| 5 | The microbiota is dispensable for the early stages of peripheral regulatory T cell induction within mesenteric lymph nodes显示文摘Intestinal Foxp3+regulatory T cell(Treg)subsets are crucial players in tolerance to microbiota-derived and food-borne antigens,and compelling evidence suggests that the intestinal microbiota modulates their generation,functional specialization,and maintenance.Selected bacterial species and microbiota-derived metabolites,such as short-chain fatty acids(SCFAs),have been reported to promote Treg homeostasis in the intestinal lamina propria.Furthermore,gut-draining mesenteric lymph nodes(mLNs)are particularly efficient sites for the generation of peripherally induced Tregs(pTregs).Despite this knowledge,the direct role of the microbiota and their metabolites in the early stages of pTreg induction within mLNs is not fully elucidated.Here,using an adoptive transfer-based pTreg induction system,we demonstrate that neither transfer of a dysbiotic microbiota nor dietary SCFA supplementation modulated the pTreg induction capacity of mLNs.Even mice housed under germ-free(GF)conditions displayed equivalent pTreg induction within mLNs.Further molecular characterization of these de novo induced pTregs from mLNs by dissection of their transcriptomes and accessible chromatin regions revealed that the microbiota indeed has a limited impact and does not contribute to the initialization of the Treg-specific epigenetic landscape.Overall,our data suggest that the microbiota is dispensable for the early stages of pTreg induction within mLNs. | Carolin Wiechers Mangge Zou Eric Galvez Michael Beckstette Maria Ebel Till Strowig Jochen Huehn Joern Pezoldt | 2021 | Cellular & Molecular Immunology2021，18，5： | 2 |
| 6 | Stromal cell-derived factor-lalpha promotes melanoma cell invasion across basement membranes involving stimulation of membrane-type 1 matrix metalloproteinase and Rho GTPase activities 显示文摘 | Bartolome RA Galvez BG Longo N | 2004 | Cancer Res2004，64，7： | 1 |
| 7 | Application of bacteriocins in the control of foodborne pathogenic and spoilage bacteria显示文摘 | GALVEZ A LOPEZ R L ABRIOUEL H | 2008 | Critical Reviews in Biotechnology2008，28，2： | 1 |
| 8 | Purification and char- acterization of a unique, potent, peptidyl probe for the high conductan- ce calcium-activated potassium channel from venom of the scorpion B- uthus tamulus显示文摘 | Galvez A Gimenez-Gallego G Reuben JP | 1990 | Journal of Biological Chemistry1990，265，11： | 1 |
| 9 | Pregabalin reduces pain and improves sleep and mooddisturbances in patients with post-herpetic neuralgia : resultsof a randomised,placebo-controlled clinical trial显示文摘 | SABATOWSKI R GALVEZ R CHERRY D A | 2004 | Pain2004，109，12： | 1 |
| 10 | Monthly cisplatin ( C ) and gemcitabine(G) as second-line chemotherapy for patients with advanced breast cancer 显示文摘 | Galvez CA Calmarini F Curie M | 2000 | Breast Cancer Res Treat2000，64，1： | 1 |
| 11 | Acinetobacter baumannii baateremin:clinical and prongostic features显示文摘 | Rodriguez BJ Pascual A Galvez J | 2003 | Euferm Infect Microbiol Clin2003，21，5： | 1 |
| 12 | Onset of breast development in a longitudinal cohort显示文摘 | Biro FM Greenspan LC Galvez MP | 2013 | Pediatrics2013，132，6： | 1 |
| 13 | Anti - inflammatory activity of diosmin and hesper- idin in rat colitis induced by TNBS显示文摘 | Crespo ME Galvez J Cruz T | 1999 | PlanMed1999，65，7： | 1 |
| 14 | Knowledge represcntation form in mechanical engineering 显示文摘 | Bello R Galvez D Sanchez G | 1994 | Knowledge - Based Systems1994，7，3： | 1 |
| 15 | Serial EEG findings in 27cases of creutzeldt Jacob Disease显示文摘 | Chiofalo N Fuentes A Galvezs | 1980 | Arch Neuro11980，37，3： | 1 |
| 16 | Splitting failure of precast prestressed con-crete during the release of the prestressing force 显示文摘 | Galvez J C Benitez J M Tork B | 2009 | Engineering Failure Analysis2009，16，8： | 1 |
| 17 | A new legged-robot configuration for research in force dis- tribution 显示文摘 | GALVEZ J A ESTREMERA J GONZALEZ P | 2003 | Mechatronics2003，13，： | 1 |
| 18 | Distinct path- ways regulate proapoptotic Nix and BNip3 in cardiac stress 显示文摘 | Galvez A S Brunskill E W Marreez Y | 2006 | J Biol Chem2006，281，3： | 1 |
| 19 | Non-intubated video- assisted ehoracic surgery management of secondary spontaneous pneu- mothorax显示文摘 | Galvez C Bolufer S Navarro-Martinez J | 2015 | Ann Transl IVied2015，3，8： | 1 |
| 20 | Transmission studies of the hoja blanca virus with highly active, virus-free colonies of Sogatodes oryzicola显示文摘 | Galvez G E | 1968 | Phytopathology1968，58，： | 1 |
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