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| 1 | 2019新型冠状病毒基因组特征和流行病学:病毒起源和受体结合的意义显示文摘研究者对来自9例新型冠状病毒肺炎住院患者的支气管肺泡灌洗液样本和培养的分离株进行了下一代测序。从这些个体中获得了严重急性呼吸综合征-冠状病毒2(severe acute respiratory syndrome-coronavirus 2,SARS-CoV-2)的完整和部分基因组序列。利用Sanger测序连接病毒重叠群以获得全长基因组,cDNA末端快速扩增确定终端区。对这些SARSCoV-2基因组和其他冠状病毒基因组进行了系统进化分析,以确定该病毒的进化史并有助于推断其可能的起源。 | 刘青(译) 刘莉(审校) Lu R Zhao X Li J Niu P Yang B Wu H Wang W Song H Huang B Zhu N Bi Y Ma X Zhan F Wang L Hu T Zhou H Hu Z Zhou W Zhao L Chen J Meng Y Wang J Lin Y Yuan J Xie Z Ma J Liu WJ Wang D Xu W Holmes EC Gao GF Wu G Chen W Shi W Tan W | 2020 | 中华高血压杂志2020，28，3： | 516 |
| 2 | Methods, units and quality requirements for the analysis of haemoglobin A_(1c) in diabetes mellitus显示文摘The formation of glycohemoglobin, especially the hemoglobin A1c(Hb_(A1c)) fraction, occurs when glucose becomes coupled with the amino acid valine in the β-chain of Hb; this reaction is dependent on the plasma concentration of glucose. Since the early 1970 s it has been known that diabetics display higher values of Hb_(A1c) because they have elevated blood glucose concentrations. Thus Hb_(A1c) has acquired a very important role in the treatment and diagnosis of diabetes mellitus. After the introduction of the first quantitative measurement of Hb_(A1c), numerous methods for glycohemoglobin have been introduced with different assay principles: From a simple minicolumn technique to the very accurate automated highpressure chromatography and lastly to many automated immunochemical or enzymatic assays. In early days, the results of the quality control reports for Hb_(A1c) varied extensively between laboratories, therefore in United States and Canada working groups(WG) of the Diabetes Controls and Complications Trial(DCCT) were set up to standardize the Hb_(A1c) assays against the DCCT/National Glycohemoglobin Standardization Program reference method based on liquid chromatography. In the 1990 s, the International Federation of Clinical Chemistry and Laboratory Medicine(IFCC) appointed a new WG to plan a reference preparation and method for the Hb_(A1c) measurement. When the reference procedureswere established, in 2004 IFCC recommended that all manufacturers for equipment used in Hb_(A1c) assays should calibrate their methods to their proposals. This led to an improvement in the coefficient of variation(CV%) associated with the assay. In this review, we describe the glycation of Hb, methods, standardization of the Hb_(A1c) assays, analytical problems, problems with the units in which Hb_(A1c) values are expressed, reference values, quality control aspects, target requirements for Hb_(A1c), and the relationship of the plasma glucose values to Hb_(A1c) concentrations. We also note that the acceptance of the mmol/mol system for Hb_(A1c) as recommended by IFCC, i.e., the new unit and reference ranges, are becoming only slowly accepted outside of Europe where it seems that expressing Hb_(A1c) values either only in per cent units or with parallel reporting of percent and mmol/mol will continue. We believe that these issues should be resolved in the future and that it would avoid confusion if mmol/mol unit for Hb_(A1c) were to gain worldwide acceptance. | Ilkka Penttil? Karri Penttil? P?ivi Holm Harri Laitinen P?ivi Ranta Jukka T?rr?nen Rainer Rauramaa | 2016 | World Journal of Methodology2016，6，2： | 3 |
| 3 | Overall efficiency of in vitro embryo production and vitrification in cattle显示文摘 | G Vajta P Holm T Greve | 1996 | Theriogenology1996，45，： | 2 |
| 4 | New method for culture of zona-included or zona-free embryos: The well of the well (WOW) system显示文摘 | G Vajta T T Peura P Holm | 2000 | Molecular Reproduction andDevelopment2000，55，： | 2 |
| 5 | Vitrification of porcine embryos using the Open Pulled Straw(OPS) method 显示文摘 | Vajta G Holm P Greve T | 1997 | Acta Vet Scand1997，38，4： | 1 |
| 6 | Management theory applications of prospect theory:Accomplishments,challenges,and opportunities显示文摘 | Holmes M.Jr Bromiley P Devers C Holcomb T &McGuire J | | 0，，04： | 1 |
| 7 | Open Pulled Straw(OPS) Vitrifica- tion: a New Way to Reduce Cryoinjuries of Bovine Ova and Embryos 显示文摘 | Vajta G Holm P Kuwayama M Booth P J Jacobsen H Greve T Callesen H | 1998 | Molecular Reproduction and Develop- ment1998，51，1： | 1 |
| 8 | Using River Habitat Survey for Environmental Assessment and Catchment Planning in the UK显示文摘 | Raven P J Holmes N T H Naura M | 2000 | Hydrobiologia2000，，： | 1 |
| 9 | Optimized space vector switching sequences for multilevel inverters显示文摘 | McGrath B P Holmes D G Lipo T A | 2003 | IEEE Transactions on Power Electronics2003，18，6： | 1 |
| 10 | Lipofection--a highly efficient, lipid-mediated DNA-transfection procedure显示文摘 | Felgner P L Gadek T R Holm M | | 0，，： | 1 |
| 11 | Effects of transitory water stress on potato tuber stem-end reducing sugar and fry color显示文摘 | Eldredge E P Holmes Z A Mosley A R Shock C C Stieber T D | | 0，，： | 1 |
| 12 | Reproductive health in humans and wildlife:Are adverse trends associated with environmental chemical exposure显示文摘 | Harrison P T C Holmes P Humfrey C D N | 1997 | Science of the Total Environment1997，205，23： | 1 |
| 13 | Reovirus apoptosis and virulence are regulated by host cell membrane penetration efficiency显示文摘 | Danthi P Kobayashi T Holm GH | 2008 | J Virol2008，82，1： | 1 |
| 14 | Optimized design of stationary frame three phase ac current regulators显示文摘 | Holmes D G Lipo T A McGrath B P | 2009 | IEEE Transactions on Power Electronics2009，24，11： | 1 |
| 15 | Losartan, an AT1 antagonist,prevents aortic aneurysm in a mouse model of Marian syndrome显示文摘 | HABASHI J P JUDGE D P HOLM T M | 2006 | Scinece2006，312，5770： | 1 |
| 16 | Activation of the ribosomal RNA genes late in the third cell cycle of porcine embryos 显示文摘 | Viuff D Greve T Holm P | 2002 | Biol Reprod2002，66，： | 1 |
| 17 | The effect of body mass in-dex on three methods of fetal weight estimation显示文摘 | Farrell T Holmes R Stone P | 2002 | BJOG2002，109，6： | 1 |
| 18 | Effect of folic acid treatment on endothelium-dependent vasodilation and nitric oxide-derived end products in hyperhomocysteinemic subjects 显示文摘 | Holven KB Holm T Aukrust P | 2001 | Am J Med2001，110，7： | 1 |
| 19 | Using river habitat survey for environmental assessment and catchment planning in the UK显示文摘 | RAVEN P J HOLMES N T H NAURA M | 2000 | Hydrobio-logia2000，，： | 1 |
| 20 | Using river habitat survey for environmental, assessment and catchment planning in the UK 显示文摘 | RAVEN P J HOLMES N T H NAURA M | 2000 | Hydrolbiologia2000，，： | 1 |
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