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1Genetic analysis and gene mapping of a narrow leaf mutant in rice (Oryza sativa L.)显示文摘A narrow leaf mutant was obtained after T-DNA transformation conducted on a rice variety Zhonghua 11. Several abnormal morphological characteristics, including semi-dwarf, delayed flowering time, narrow and inward rolling leaves, and lower seed-setting, were observed. The rate of net photosynthesis (un-der saturate light) of flag leaves in the mutant was significantly lower than that of the wild type. More-over, the leaf transpiration rate and stomatal conductance in the mutant flag leaf were lower than those of the wild type at the grain filling stage. It was found that the mutant phenotype was not caused by the T-DNA insertion. Genetic analysis showed that the mutant was controlled by a single recessive gene, designated as nal3(t). A genetic linkage map was constructed using a large F2 mapping population de-rived from a cross between nal3(t) and an indica variety Longtefu B with 6 polymorphic markers on chromosome 12 identified from 366 SSR markers by the BAS method. Gene nal3(t) was mapped be-tween the markers RM7018 and RM3331. Fine mapping of nal3(t) locus was conducted with 22 newly developed STS markers based on the sequence diversity around the region harboring nal3(t) between Nipponbare and 93-11, and nal3(t) was finally mapped to a 136-kb region between the STS markers NS10 and RH12-8.WANG DeKai LIU HeQin LI KeLei LI SuJuan TAO YueZhi 2009Chinese Science Bulletin2009,54,5:19
2Impact of anthropogenic drivers on subaqueous topographical change in the Datong to Xuliujing reach of the Yangtze River显示文摘Changes of subaqueous topography in shallow offshore water pose safety risks for embankments,navigation,and ports.This study conducted measurements of subaqueous topography between Datong and Xuliujing in the Yangtze River using a Sea Bat 7125 multi-beam echo sounder,and the channel change from 1998 to 2013 was calculated using historical bathymetry data.The study revealed several important results:(1)the overall pattern of changes through the studied stretch of the river was erosion–deposition–erosion.Erosion with a volume 700×10~6m^3occurred in the upper reach,deposition of about 204×10~6m^3occurred in the middle reach,and erosion of about 602×10~6m^3occurred in the lower reach.(2)Dunes are the most common microtopographic feature,accounting for 64.3%of the Datong to Xuliujing reach,followed by erosional topography and flat river topography,accounting for 27.6%and 6.6%,respectively.(3)Human activities have a direct impact on the development of the microtopography.For instance,the mining of sand formed holes on the surface of dunes with lengths of 20–35 m and depths of 3–5 m.We concluded that the overall trend of erosion(net erosion volume of 468×10~6m^3)occurred in the study area mainly because of the decreased sediment discharge following the closure of the Three Gorges Dam.However,other human activities were also impact factors of topographic change.Use of embankments and channel management reduced channel width,restricted river meandering,and exacerbated the erosion phenomenon.Shuwei ZHENG Heqin CHENG Shengyu SHI Wei XU Quanping ZHOU Yuehua JIANG Fengnian ZHOU Minxiong CAO 2018Science China Earth Sciences2018,61,7:10
3Morphological evolution of mouth bars on the Yangtze estuarine waterways in the last 100 years显示文摘在 Yangtze 河口水路的嘴酒吧在河口,洪水分泌物和上海港口的建设以内在航行运输上有重要影响。在这份报纸, Yangtze 河口水路的嘴酒吧形成的词法进化和机制被分析几百年历史的数据学习,一些或主要的嘴的最近的侧面地图在 Yangtze 河口锁隧道。结果如下被显示出:在北方布朗奇的嘴酒吧从在嘴外面逐渐地搬到里面并且形成了一个巨大的沙酒吧。在北方隧道,嘴酒吧的头下游地移植了大约 30 公里,并且自从 2001,一个隧道酒吧发展中。二个嘴酒吧顶,总是在北方经过存在,消失在 2010。在南方经过的嘴酒吧的头下游地移植了大约 14 km,顶的数字起初增加了,但是现在被归结为仅仅。根据结果,我们能断定嘴酒吧的进化不同取决于他们的地点。在它是的北方布朗奇,直接,与在 Chongming 岛的大规模开垦有关,但是在诺思经过,它与 Yangtze 河口深海的隧道的规定有一种靠近的关系。然而,在北方隧道和南方经过的嘴酒吧的进化是不仅在浅的东方 Hengsha 和 Qingcaosha 水库的闭合与 Yangtze 河口深海的隧道规定工程,而且与开垦连接了。HE Yufang CHENG Heqin CHEN Jiyu 2013Journal of Geographical Sciences2013,23,2:9
4OspTAC2 encodes a pentatricopeptide repeat protein and regulates rice chloroplast development显示文摘Functional chloroplast generation depends on the precise coordination of gene expression between the plastid and the nucleus and is essential for plant growth and development. In this study, a rice(Oryza sativa) mutant that exhibited albino and seedling-lethal phenotypes was isolated from a60Co-irradiated rice population. The mutant gene was identified as an ortholog of the Arabidopsis plastid transcriptionally active chromosome protein 2(p TAC2) gene, and the mutant strain was designated osptac2. Sequence and transcription analyses showed that Osp TAC2 encodes a putative chloroplast protein consisting of 10 pentratricopeptide repeat(PPR) domains and a C-terminal small Mut S-related(SMR) domain. Cytological observations via microscopy showed that the Osp TAC2-green fluorescent fusion protein is localized in the chloroplasts. Transmission electron microscopy revealed that the chloroplast of the osptac2 mutant lacks an organized thylakoid membrane. The transcript levels of all investigated PEP(plastid-encoded RNA polymerase)-dependent genes were dramatically reduced in the osptac2 mutant, whereas the transcript levels of NEP(nuclear-encoded polymerase)-dependent genes were increased. These results suggest that Osp TAC2 plays a critical role in chloroplast development and indicate that the molecular function of the Osp TAC2 gene is conserved in rice and Arabidopsis.Dekai Wang Heqin Liu Guowei Zhai Liangsheng Wang Jianfeng Shao Yuezhi Tao 2016Journal of Genetics and Genomics2016,43,10:7
5The self-regulation process and its mechanism of channels' bed changes in the Changjiang(Yangtze) Estuary in China显示文摘Recent bathymetric changes in the Changjiang Estuary under the influence of artificial regulation engineerings and basin reservoirs have been analyzed based on the maritime charts since 1997 and recent fieldworks. The results indicate a slight erosion of the channels in the upper and middle estuary, continuing deposition and seaward move of the mouth bar crest and intensifying erosion at the nearshore seabed. It is noteworthy that the morphological evolution caused by intensive human activities dominates over the changes from nature process.First, the riverbes are eroded overall in the South Branch(SB), the South Channel(SC) and the upper and middle reaches of the North Channel(NC). The nearshore seabed outside the river mouth is being eroded slightly, which is attributed to the declining sediment supply from the Changjiang Basin due to the construction of the Three Gorges Dam upstream. The sediment above the seabed is very active and coarsened, meanwhile, sand waves are becoming more distinct. Second, a deposition occurs in the North Brach(NB), the mouth of the NC, the mouth bars of the North Passage(NP) and the South Passage(SP) and especially the main channel of the NP, where it shows a massive siltation after the deep waterway project. The reasons for the recent changes are not only the dynamic structure in estuarine mouth bars, but also the supply of sediment resuspension in a local and offshore area. Meanwhile, the severe erosion and siltation in some reaches is related to the construction of estuarine engineerings. It is indicated that the Changjiang Estuary is gradually self-adjusted and adapting to the varying natural factors and intensive human activities. The study on the mechanism of self-regulation of the recent bathymetric changes in the Changjiang Estuary has important and practical significance.ZHANG Xiaohe LI Jiufa ZHU Wenwu CHENG Heqin CHEN Wei 2015Acta Oceanologica Sinica2015,34,7:5
6Characterization and molecular cloning of a serine hydroxymethyltransferase 1(OsSHM_1) in rice显示文摘Serine hydroxymethyltransferase(SHMT) is important for one carbon metabolism and photorespiration in higher plants for its participation in plant growth and development,and resistance to biotic and abiotic stresses. A rice serine hydroxymethyltransferase gene, Os SHM1, an ortholog of Arabidopsis SHM1, was isolated using map-based cloning. The osshm1 mutant had chlorotic lesions and a considerably smaller,lethal phenotype under natural ambient CO2 concentrations,but could be restored to wild type with normal growth under elevated CO2levels(0.5% CO2), showing a typical photorespiratory phenotype. The data from antioxidant enzymes activity measurement suggested that osshm1 was subjected to signi fi cant oxidative stress. Also, Os SHM1 was expressed in allorgans tested(root, culm, leaf, and young panicle) but predominantly in leaves. Os SHM1 protein is localized to the mitochondria. Our study suggested that molecular function of the Os SHM1 gene is conserved in rice and Arabidopsis.Dekai Wang Heqin Liu Sujuan Li Guowei Zhai Jianfeng Shao Yuezhi Tao 2015Journal of Integrative Plant Biology2015,57,9:3
7Sand mining impact on Poyang Lake: a case study based on high-resolution bathymetry and sub-bottom data显示文摘Poyang Lake in the Changjiang(Yangtze)River catchment has undergone frequent spring drought since 2003,and some researchers attributed this phenomenon to sand mining and the lakebed deformation in the outlet channel linking the lake with Changjiang River main channel.However,there is still a lack of high-resolution subaqueous geomorphological evidence of how sand mining led to lakebed deformation in the outlet channel.We examined the bed morphology and sub-bottom sedimentary structure of the outlet channel,using a multibeam echo sounder and sub-bottom profi ler in Poyang Lake.We found that:(1)the subaqueous micro-topography types of the outlet channel are characterized by sand mining disturbance,natural erosional topography,and fl at bed and dunes,accounting for 44.9%,21.4%,28.6%,and 5.1%of the channel area,respectively;and(2)sand mining activity aff ects the local bed topography extensively and signifi cantly.The depth of sandpits caused by sand mining varied from 1.4 m to 12 m deeper than the surrounding bed surface,with 4.41 m of depth increase on average.Hence,the large-scale high-intensity sand mining activities and their signifi cant geomorphic eff ects demand for an improved assessment for future management and longer-term sustainability.Because of the large-scale and ongoing high-intensity sand mining activities in the Poyang Lake outlet channel,these eff ects should raise caution in the future and contribute to monitoring eff orts that are essential to implement sustainable management solutions.The present study and techniques implemented can serve as a scientifi c reference for dam construction and sand mining within the Poyang Lake basin.Shuwei ZHENG Heqin CHENG Ming TANG Wei XU Enfeng LIU Shu GAO Jim BEST Yuehua JIANG Quanping ZHOU 2022Journal of Oceanology and Limnology2022,40,4:3
8Succinate dehydrogenase SDH1–1 positively regulates cotton resistance to Verticillium dahliae through a salicylic acid pathway显示文摘Background:Verticillium wilt,caused by the soil-borne fungus of Verticillium dahliae Kleb.,is one of the most devastating diseases of cotton.The complex mechanism underlying cotton resistance to Verticillium wilt remains uncharacterized.Identifying an endogenous resistance gene may be helpful to control this disease.Previous studies revealed that succinate dehydrogenase(SDH)is involved in reactive oxygen species(ROS)-induced stress signaling pathway that is likely to be triggered by salicylic acid(SA).Here,through the metabolomics and differential expression analyses in wilt-inoculated cotton(Gossypium hirsutum),we noticed that Gh SDH1–1 gene in cotton may play an important role in the resistance to V.dahlia.Then we reported Gh SDH1–1 gene and its functional analysis in relation to the resistance of cotton to V.dahliae.Results:The Gh SDH1–1 gene in cotton root was significantly up-regulated after V.dahlia inoculation,and its expression level peaked at 12 and 24 h post-infection.SA can also induce the up-regulation of Gh SDH1–1.Additionally,the functional analysis showed that Gh SDH1–1-silenced cotton was more susceptible to V.dahliae than the control because of the significant decrease in abundance of immune-related molecules and severe damage to the SA-signaling pathway.In Arabidopsis thaliana,high expression of Gh SDH1–1 conferred high resistance to V.dahliae.Arabidopsis that overexpressed Gh SDH1–1 had higher resistance to V.dahliae infection compared with the wild-type.Conclusions:Our findings provide new insights into the role of Gh SDH1–1;it positively regulates cotton resistance to Verticillium wilt.The regulatory mechanism of Gh SDH1–1 is closely related to SA-related signaling pathway.ZHANG Xiangyue FENG Zili ZHAO Lihong LIU Shichao WEI Feng SHI Yongqiang FENG Hongjie ZHU Heqin 2020Journal of Cotton Research2020,3,2:3
9Nonlinear advection,Coriolis force,and frictional influence in the South Channel of the Yangtze Estuary,China显示文摘Observation data of along-estuary and lateral current velocities over a transect located at the South Channel of the Yangtze estuary was obtained during a spring tide in August 2011.Harmonic analysis was done on the current velocities to get a mean component and a semi-diurnal component.Based on these two components,the driving mechanisms of mean lateral flow and M2 lateral tidal flow are shown and analyzed respectively.The dominant driving force of mean lateral flow is nonlinear advection and that of lateral M2 tidal flow is Coriolis force.The friction plays an important role near the bottom and surface for both lateral mean flow and M2 tidal flow.YANG ZhongYong CHENG HeQin LI JiuFa 2015Science China Earth Sciences2015,58,3:2
10Fluctuations in the tidal limit of the Yangtze River estuary in the last decade显示文摘The tidal limit is the key interface indicating whether water levels will be affected by tidal waves, which is of great significance to navigation safety and regional flood control. Due to limitations in research methods, recent changes in the Yangtze River tidal limit, caused by sea level rise and large-scale engineering projects, urgently need to be studied. In this study,spectrum analysis was undertaken on measured water level data from downstream Yangtze River hydrological stations from2007 to 2016. The bounds of the tidal limit were identified through comparisons between the spectra and red noise curves, and the fluctuation range and characteristics were summarized. The results showed that:(1) During the extremely dry period, when the flow rate at Jiujiang station was about 8440 m3 s-1, the tidal limit was near Jiujiang; whereas during the flood season, when the flow rate at Jiujiang station was about 66700 m3 s-1, the tidal limit was between Zongyang Sluice and Chikou station.(2)From the upper to lower reach, the effect of the Jiujiang flow rate on the tidal limit weakens, while the effect of the Nanjing tidal range increases. The tidal limit fluctuates under similar flow rates and tidal ranges, and the fluctuation range increases with increasing flow rate and decreasing tidal range.(3) With the continued influence of rising sea levels and construction in river basin estuaries, the tidal limit may move further upstream.Shengyu SHI Heqin CHENG Xiaona XUAN Fangxi HU Xiaoting YUAN Yuehua JIANG Quanping ZHOU 2018Science China Earth Sciences2018,61,8:2
11A review of the pathogenicity mechanism of Verticillium dahliae in cotton显示文摘Verticillium wilt,caused by the notorious fungal pathogen Verticillium dahliae,is one of the main limiting factors for cotton production.Due to the stable dormant structure microsclerotia,long-term variability and co-evolution with host plant,its pathogenicity mechanism is very complicated,and the interaction mechanism between pathogen and host plant is also unclear.So identification and functional analysis of the genes involved in the pathogenicity or virulence of this fungus will benefit to uncover the molecular pathogenic mechanism of V.dahliae.In this review,many multifunction genes covering microsclerotia development,pathogen infection,effector proteins,transcription factors,horizontal gene transfer and trans-kingdom RNA silencing have been summarized to provide a theoretical basis to deep understand the molecular pathogenicity mechanism of V.dahliae and promote to effectively control Verticillium wilt.Furtherly,these pathogenicity-related genes may be considered as targets for effective control of Verticillium wilt in cotton.ZHANG Yalin ZHOU Jinglong ZHAO Lihong FENG Zili WEI Feng BAI Hongyan FENG Hongjie ZHU Heqin 2022Journal of Cotton Research2022,5,1:2
12Recent progress on the modification of high nickel contentNCM:Coating,doping,and single crystallization显示文摘High nickel content layered cathodes,represented by NCM(LiNi_(x)Co_(y)Mn_(z)O_(2),x+y+z=1),are now widely employed in the market of electric vehicles,owing to their high energy density.With the gradual increase of nickel content and capacity,the issues on cycling life and safety become more serious.In this review,various strategies for improving the performance of high nickel NCM are summarized on the aspects of surface coating,ionic doping,and singlecrystal NCM.The coating strategy was separately described according to the physical property of coating species,including inert material coating,Li^(+)-conductor coating,electronic conductor coating,and mixed conductor coating.These coating species help to suppress the interfacial oxidation of electrolytes by NCM,improving the cycling life and safety.The elemental doping in the crystal lattice of NCM is then presented in the aspects of cation,anion,and mixed-ion doping,which are beneficial to stabilize the layered structure during charge–discharge and so promote the electrochemical performance.In quite recent years,the strategy of single-crystal NCM was demonstrated to be a promising pathway,owing to the dramatically reduced surface area and grain boundary.Finally,the remaining unsolved challenges and future strategies for further development of NCM cathode materials are outlined.Junqing Yan Hao Huang Junfan Tong Wei Li Xiaohang Liu Haoxuan Zhang Heqin Huang Weidong Zhou 2022Interdisciplinary Materials2022,1,3:2
13Transalkyla- tion of diisopropylbenzenes with benzene over hierarchical beta zeolite显示文摘Yang Heqin Liu Zhicheng Gao Huanxin 2010Applied Catalysis A General2010,379,12:1
14Synthesis and catalytic performances of hierarchical SAPO-34 monolith显示文摘Yang Heqin Liu Zhicheng Gao Huanxin 2010Journal of Materials Chernistry2010,20,16:1
15Transalkyla- tion of di-isopropylbenzenes with benzene over hierarchical beta zeolite 显示文摘Yang Heqin Liu Zhicheng Gao Huanxin 2010Applied Catalysis A : General2010,379,:1
16Frequency and distribution of genome-based microsatellites in Ver- ticillium dahliae 显示文摘Li Zhifang Zhu Heqin Zhao Lihong 2012African Journal of Microbiology Research2012,6,49:1
17Synthesis of 5-(5-nitro-2-pyridylazo)-2, 4-Diaminotoluene and its Application to Spectrophotornetric Determination of Microamounts of Palladium显示文摘Yang Heqin Zhang Gang Zhang Linlin 1996Talanta1996,43,:1
18Synthesis of pyridine and picolines over Co - modified HZSM - 5 catalyst 显示文摘Yumin Heqin Yang Fang Jin Yue Zhang Yongdan Li 2008Chemical Engineering Journal2008,136,:1
19Efficacy evaluation and mechanism of Bacillus subtilis EBS03 against cotton Verticillium wilt显示文摘Background:In our previous study,a strain EBS03 with good biocontrol potential was screened out of 48 strains of cotton endophyte Bacillus subtilis by evaluating the controlling effect against cotton Verticillium wilt.However,its mechanism for controlling Verticillium wilt remains unclear.The objective of this study was to further clarify its con-trolling effect and mechanism against cotton Verticillium wilt.Results:The results of confrontation culture test and double buckle culture test showed that the inhibitory effects of EBS03 volatile and nonvolatile metabolite on mycelium growth of Verticillium dahliae were 70.03%and 59.00%,respectively;the inhibitory effects of sporulation and microsclerotia germination were 47.16%and 70.06%,respec-tively.In the greenhouse test,the EBS03 fermentation broth root irrigation had the highest controlling effect at 87.11%on cotton Verticillium wilt,and significantly promoted the growth of cotton seedlings.In the field experi-ment,the controlling effect of EBS03 fermentation broth to cotton Verticillium wilt was 42.54%at 60 days after cotton sowing,and the boll number per plant and boll weight in EBS03 fermentation broth seed soaking,root irrigation,and spraying treatments significantly increased by 19.48%and 7.42%,30.90%and 2.62%,15.99%and 9.20%,respec-tively.Furthermore,EBS03 improved the resistance of cotton leaves against the infection of V.dahliae,and induced the outbreak of reactive oxygen species and accumulation of callose.In addition,the results of real time fluorescent quantitative polymerase chain reaction(RT-qPCR)detection showed that EBS03 significantly induced upregulation expression level of defense-related genes PAL,POD,PPO,and PR10 in cotton leaves,enhanced cotton plant resistance to V.dahliae,and inhibited colonization level of this fungal pathogen in cotton.Conclusion:Bacillus subtilis EBS03 has a good biological defense capability,which can inhibit the growth and coloni-zation level of V.dahliae,and activate the resistance of cotton to Verticillium wilt,thus increase cotton yield.BAI Hongyan FENG Zili ZHAO Lihong FENG Hongjie WEI Feng ZHOU Jinglong GU Aixing ZHU Heqin PENG Jun ZHANG Yalin 2022Journal of Cotton Research2022,5,4:1
20Characterization and Performance of V2O5-TiO2 Catalysts Prepared by Rapidcombustion Method 显示文摘Heqin Guo Debao Li Dong Jiang 2010Catalysts Today2010,158,:1
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