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| 1 | SARS-CoV-2 spike protein binds to bacterial lipopolysaccharide and boosts proinflammatory activity显示文摘There is a link between high lipopolysaccharide(LPS)levels in the blood and the metabolic syndrome,and metabolic syndrome predisposes patients to severe COVID-19.Here,we define an interaction between SARS-CoV-2 spike(S)protein and LPS,leading to aggravated inflammation in vitro and in vivo.Native gel electrophoresis demonstrated that SARS-CoV-2 S protein binds to LPS.Microscale thermophoresis yielded a of〜47 nM for the interaction.Computational modeling and all-atom molecular dynamics simulations further substantiated the experimental results,identifying a main LPS-binding site in SARS-CoV-2 S protein.S protein,when combined with low levels of LPS,boosted nuclear factor-kappa B(NF-k B)activation in monocytic THP-1 cells and cytokine responses in human blood and peripheral blood mononuclear cells,respectively.The in vitro inflammatory response was further validated by employing NF-kB reporter mice and in vivo bioimaging.Dynamic light scattering,transmission electron microscopy,and LPS-FITC analyses demonstrated that S protein modulated the aggregation state of LPS,providing a molecular explanation for the observed boosting effect.Taken together,our results provide an interesting molecular link between excessive inflammation during infection with SARS-CoV-2 and comorbidities involving increased levels of bacterial endotoxins. | Ganna Petruk Manoj Puthia Jitka Petrlova Firdaus Samsudin Ann-Charlotte Stromdahl Samuel Cerps Lena Uller Sven Kjellstrom Peter J.Bond Artur Schmidtchen | 2020 | Journal of Molecular Cell Biology2020，12，12： | 3 |
| 2 | Effect of sildena- fil on haemodynamic parameters of pulmonary circulation and physical capacity in patients with systolic chronic heart failure with secondary severe pulmonary hypertension 显示文摘 | Josiak K Inorowicz E Petruk Kowalczyk J | 2011 | Kardiol Pol2011，69，5： | 1 |
| 3 | Nimodipine treatment in poor-grade aneurysm patients 显示文摘 | Petruk KC West M Mohr G | 1988 | J Neurosurg1988，68，4： | 1 |
| 4 | Glioma immunology and immunotherapy显示文摘 | Pamey IF Han C Petruk KC | 2000 | Neatrosurgery2000，46，4： | 1 |
| 5 | Glioma immunology and inuramotherapy 显示文摘 | Parney IF Hao C Petruk KC | 2000 | Neurosmgery2000，46，4： | 1 |
| 6 | Geochemistry of secondgry copper minerals from Proterozoic porphyry copper deposit,Malanjkhand,India显示文摘 | Petruk William Nehru Cherukupalli E Zhang Zheru | 1991 | Ore Geology Reviews1991，6，23： | 1 |
| 7 | Computed tomography angiography versus digital subtraction angiography for the diagnosis and early tretment of ruptured intracranial aneursms显示文摘 | Anderson GB Steinke DE Petruk KC | 1999 | Neurosurgery1999，45，： | 1 |
| 8 | Glioma immunology and immunotherapy 显示文摘 | Hao CH Petruk KC | 2000 | Neurosurgery2000，46，： | 1 |
| 9 | Computed tomo-graphy in cranniography versus digital subtraction angiographyfor the diagnosis and early treatment of ruptured intracranialaneurysms显示文摘 | Anderson GB Steinke DE Petruk KC | 1999 | Neurosurgery1999，45，6： | 1 |
| 10 | Glioma immunology and immunotherapy 显示文摘 | Pamey IF Chunhai Hao Petruk KC | 2000 | Neurosurgery2000，46，77： | 1 |
| 11 | Computed tomographic angiography versus digital subtraction angiography for t he diagnosis and early treatment of ruptured intracrsuial aneurysma显示文摘 | Anderson CB Steinke DE Petruk KC | 1999 | Neuro-surgery1999，45，6： | 1 |
| 12 | Electron microprobe analyses for pyrochlores from Oka, Quebec 显示文摘 | Petruk W De'Alton R O | 1975 | The Canadian Mineralogist1975，15，28： | 1 |
| 13 | Ecdysone-and no-mediated gene regulation by competing EcR/UsP and E75A nuclear receptors during Drosophila development显示文摘 | Johnston DM Sedkov Y Petruk S Riley KM Fujioka M Jaynes JB | | 0，，： | 1 |
| 14 | Glioma immunology and immunotherapy显示文摘 | Hao CH Petruk KC | 2000 | Neurosurgery2000，46，4： | 1 |
| 15 | GM-CSF and B7-2 combination imm unogene therapy in and allogeneic hu-PBL-SCID/beige mouse:Human glioblast oma multiforme model显示文摘 | Petruk KC Zhang C | 1997 | Hum Gene Ther1997，8，3： | 1 |
| 16 | Ecdysone- and NO-Mediated Gene Regulation by Competing EcR/Usp and E75A Nuclear Receptors during Drosophila Development显示文摘 | Danika M. Johnston Yurii Sedkov Svetlana Petruk Kristen M. Riley Miki Fujioka James B. Jaynes Alexander Mazo | 2011 | Molecular Cell2011，，1： | 1 |
| 17 | Glioma immunology and immunotherapy 显示文摘 | Parney IF Hao C Petruk KC | 2000 | Neurosurgery2000，46，4： | 1 |
| 18 | Glioma immunology and immunotherapy 显示文摘 | Parney LF Hao C Petruk KC | 2000 | Neurosurgery2000，46，4： | 1 |
| 19 | Effect of silde- nafil on haemodynamic parameters of pulmonary circulation and physical capacity in patients with systolic chronic heart failure with secondary severe pulmonary hypertension 显示文摘 | Josiak K Inorowicz E Petruk Kowalczyk J | 2011 | Kardiol Pol2011，69，5： | 1 |
| 20 | Granulocyte-macrophage colony-stimulating factor and B7-2 combination immunogene therapy in an allogeneic Hu-PBL-SCID/beige mouse-human glioblastoma multiforme model显示文摘 | Petruk KC Zhang C | 1997 | Hum Gene Ther1997，8，9： | 1 |
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