|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Washed microbiota transplantation vs.manual fecal microbiota transplantation:clinical findings,animal studies and in vitro screening显示文摘Fecal microbiota transplantation(FMT)by manual preparation has been applied to treat diseases for thousands of years.However,this method still endures safety risks and challenges the psychological endurance and acceptance of doctors,patients and donors.Population evidence showed the washed microbiota preparation with microfiltration based on an automatic purification system followed by repeated centrifugation plus suspension for three times significantly reduced FMT-related adverse events.This washing preparation makes delivering a precise dose of the enriched microbiota feasible,instead of using the weight of stool.Intraperitoneal injection in mice with the fecal microbiota supernatant obtained after repeated centrifugation plus suspension for three times induced less toxic reaction than that by the first centrifugation following the microfiltration.The toxic reactions that include death,the change in the level of peripheral white blood cells,and the proliferation of germinal center in secondary lymphoid follicles in spleen were noted.The metagenomic next-generation sequencing(NGS)indicated the increasing types and amount of viruses could be washed out during the washing process.Metabolomics analysis indicated metabolites with pro-inflammatory effects in the fecal microbiota supernatant such as leukotriene B4,corticosterone,and prostaglandin G2 could be removed by repeated washing.Near-infrared absorption spectroscopy could be served as a rapid detection method to control the quality of the washingprocess.In conclusion,this study for the first time provides evidence linking clinical findings and animal experiments to support that washed microbiota transplantation(WMT)is safer,more precise and more quality-controllable than the crude FMT by manual. | Ting Zhang Gaochen Lu Zhe Zhao Yafei Liu Quan Shen Pan Li Yaoyao Chen Haoran Yin Huiquan Wang Cicilia Marcella Bota Cui Lei Cheng Guozhong Ji Faming Zhang | 2020 | Protein & Cell2020，11，4： | 44 |
| 2 | Mesoporous silica nanoparticles for drug and gene delivery显示文摘Mesoporous silica nanoparticles(MSNs) are attracting increasing interest for potential biomedical applications. With tailored mesoporous structure, huge surface area and pore volume,selective surface functionality, as well as morphology control, MSNs exhibit high loading capacity for therapeutic agents and controlled release properties if modified with stimuli-responsive groups, polymers or proteins. In this review article, the applications of MSNs in pharmaceutics to improve drug bioavailability, reduce drug toxicity, and deliver with cellular targetability are summarized. Particularly,the exciting progress in the development of MSNs-based effective delivery systems for poorly soluble drugs, anticancer agents, and therapeutic genes are highlighted. | Yixian Zhou Guilan Quan Qiaoli Wu Xiaoxu Zhang Boyi Niua Biyuan Wu Ying Huang Xin Pan Chuanbin Wu | 2018 | Acta Pharmaceutica Sinica B2018，8，2： | 31 |
| 3 | Isolation and Identification of Cancer Stem-Like Cells from Murine Melanoma Cell Lines显示文摘In current study,cancer stem-like cells in the murine melanoma B16F10 cells were investigated.CD phenotypes of the B16F10 cells were analyzed by flow cytometry,and the specific CD phenotype cells from the B16F10 cells were isolated by MACS.Then we used colony formation assay in soft agar media,the cell growth assay in serum-free culture media as well as the tumorigenicity investigation of the specific CD phenotype cells in C57BL/6 mice, respectively,to identify cancer stem-like cells in the B16F10 cells.The results showed that the B16F10 cells could form spherical clones in serum-free culture media,and the rate of clonegenesis of CD133 + ,CD44 + and CD44 + CD133 + cells was higher than that of CD133 - ,CD44 - and CD44 + CD133 - cells in soft agar media,respectively. The tumorigenic potential of CD133 + ,CD44 + ,CD44 + CD133 + cells and CD44 + CD133 + CD24 + cells was stronger than that of CD133 - ,CD44 - ,CD44 + CD133 - cells and CD44 + CD133 + CD24 - cells in mice,respectively.In conclusion,the CD44 + CD133 + CD24 + cells have some biological properties of cancer stem-like cells or are highly similar to the characteristics of cancer stem cells(CSC).These results provide an important method for identifying cancer stem-like cells in B16F10 cells and for further cancer target therapy. | Jun Dou Meng Pan Ping Wen Yating Li Quan Tang Lili Chu Fengshu Zhao Chuilian Jiang Weihua Hu Kai Hu Ning Gu | 2007 | Cellular & Molecular Immunology2007，4，6： | 25 |
| 4 | Experimental and clinical study of influence of high-frequency electric surgical knives on healing of abdominal incision显示文摘瞄准:在腹壁切口愈合上学习高周波的电的外科的刀的影响。方法:240 只白鼠被划分成 10 (0 ) , 10 (2 ) , 10 (5 ) ,并且 10 (8 ) 组和腹部手术的老鼠模型被分别地使用电的外科的刀和普通口针劝诱。然后,他们分别地被给生理盐水和 0.2 mL 的皮下注射在 10 的集中(2 ) 的埃希氏杆菌属关口 i,葡萄球菌 aureus 和绿脓假单胞菌的量的混合, 10 (5 ) 并且 10 (8 ) 。根据动物实验,经历腹部手术(上面的类型 II ) 的 220 个病人随机被分配进跟随三个组之一:电热刀(EK, 93 个盒子) ,电凝法(EC, 55 个盒子) 并且控制(72 个盒子) 。高周波的电的外科的刀习惯于为在 EK 的血壅滞的腹的纸巾和电凝法组织的 dissect。普通口针和电凝法在 EC 组被使用。普通口针和系住的丝缝线在控制被使用。结果:在除了组 10 的所有组(0 ) ,电的外科的刀做的 incisional 创伤的感染率与普通口针比那显著地高。而且,在组有有效差量 10 (2 ) , 10 (5 ) ,并且 10 (8 )(P<0.05 ) ,然而并非(0 )(P>0.05 ) 在组, 10 在 EK 和 EC 之间组织。临床的研究显示出在控制组在 EK,在 EC 的 11 个盒子(16.36%) 和 2 个盒子(2.86%) 在 16 种情况(17.20%) 中愈合的一处推迟的创伤。在 EK 和控制之间的有效差量组织(chi2 = 8.57, P<0.01 ) ,并且在 EC 和控制组之间(chi2 = 5.66, P<0.05 ) 被观察,然而并非在 EK 和 EC 之间(chi2 = 0.017, P>0.05 ) 。结论:高周波的电的刀可以显著地推迟腹壁切口愈合。它的申请应该被最小化以便减少手术后的复杂并发症的可能性。 | Guang-Wei Ji Yuan-Zhi Wu Xu Wang Hua-Xiong Pan Ping Li Wan-Ying Du Zhi Qi An Huang Li-Wei Zhang Li Zhang Wen Chen Guang-Hua Liu Hui Xu Quan Li Ai-Hua Yuan Xiao-Ping He Guo-Hua Mei | 2006 | World Journal of Gastroenterology2006，12，25： | 23 |
| 5 | Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1显示文摘Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid peroxidation.Currently,the therapeutic role of ferroptosis on cancer is gaining increasing interest.Baicalin an active component in Scutellaria baicalensis Georgi with anticancer potential various cancer types;however,the effects of baicalein on bladder cancer and the underlying molecular mechanisms remain largely unknown.In the study,we investigated the effect of baicalin on bladder cancer cells5637 and KU-19-19.As a result,we show baicalin exerted its anticancer activity by inducing apoptosis and cell death in bladder cancer cells.Subsequently,we for the first time demonstrate baicalin-induced ferroptotic cell death in vitro and in vivo,accompanied by reactive oxygen species(ROS) accumulation and intracellular chelate iron enrichment.The ferroptosis inhibitor deferoxamine but not necrostatin-1,chloroquine(CQ),N-acetyl-L-cysteine,L-glutathione reduced,or carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone(Z-VAD-FMK) rescued baicalin-induced cell death,indicating ferroptosis contributed to baicalin-induced cell death.Mechanistically,we show that ferritin heavy chain1(FTH1) was a key determinant for baicalin-induced ferroptosis.Overexpression of FTH1 abrogated the anticancer effects of baicalin in both 5637 and KU19-19 cells.Taken together,our data for the first time suggest that the natural product baicalin exerts its anticancer activity by inducing FTH1-dependent ferroptosis,which will hopefully provide a prospective compound for bladder cancer treatment. | Na Kong Xiaying Chen Jiao Feng Ting Duan Shuiping Liu Xueni Sun Peng Chen Ting Pan Lili Yan Ting Jin Yu Xiang Quan Gao Chengyong Wen Weirui Ma Wencheng Liu Mingming Zhang Zuyi Yang Wengang Wang Ruonan Zhang Bi Chen Tian Xie Xinbing Sui Wei Tao | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 23 |
| 6 | The Association between Lower Incisal Inclination and Morphology of the Supporting Alveolar Bone—A Cone-Beam CT Study显示文摘Aim To investigate the relationship between the positioning of the lower central incisor and physical morphology of the surrounding alveolar bone. Methodology Thirty-eight patients (18 males, 20 females), with mean age of 13.4 years, were included in this study. As part of orthodontic treatment planning the patients were required to take dental Cone-beam CT (CBCT) covering the region of lower incisors, the surrounding alveolar bone and the mandibular symphysis. The cephalometric parameters were designed and measured to indicate the inclination of lower central incisor and physical morphology of the adjacent alveolar bone. Computer-aided descriptive statistical analysis was performed using SPSS 15.0 software package for Windows. A correlation analysis and a linear regression analysis between the incisor inclination and the alveolar bone morphology were performed. Results Significant positive correlations were found between the lower central incisor inclination and the morphological contour of the alveolar bone (P<0.05). The lower central incisor root apex was closer to the lingual alveolar crest when it was buccally inclined. Conclusion The morphology of the alveolar bone may be affected by incisal inclination. | Quan Yu Xiao-gang Pan Guo-ping Ji Gang Shen | 2009 | International Journal of Oral Science2009，1，4： | 19 |
| 7 | Natural Variation in CCD4 Promoter Underpins Species-Specific Evolution of Red Coloration in Citrus Peel显示文摘Carotenoids and apocarotenoids act as phytohormones and volatile precursors that influence plant development and confer aesthetic and nutritional value critical to consumer preference.Citrus fruits display considerable natural variation in carotenoid and apocarotenoid pigments.In this study,using an integrated genetic approach we revealed that a 5;c/s-regulatory change at CCD4b encoding CAROTENOID CLEAVAGE DIOXYGENASE 4b is a major genetic determinant of natural variation in C3 0 apocarotenoids responsible for red coloration of citrus peel.Functional analyses demonstrated that in addition the known role in synthesizing 3-citraurin,CCD4b is also responsible for the production of another important C3 0 apocarotenoid pigment,p-citraurinene.Furthermore,analyses of the CCD4b promoter and transcripts from various citrus germplasm accessions established a tight correlation between the presence of a putative 5'c/s-regulatory enhancer within an MITE transposon and the enhanced allelic expression of CCD4b in C3 0 apocarotenoid-rich red-peeled accessions.Phylogenetic analysis provided further evidence that functional diversification of CCD4b and naturally occurring variation of the CCD4b promoter resulted in the stepwise evolution of red peels in mandarins and their hybrids.Taken together,our findings provide new insights into the genetic and evolutionary basis of apocarotenoid diversity in plants,and would facilitate breeding efforts that aim to improve the nutritional and aesthetic value of citrus and perhaps other fruit crops. | Xiongjie Zheng Kaijie Zhu Quan Sun Weiyi Zhang Xia Wang Hongbo Cao Meilian Tan Zongzhou Xie Yunliu Zeng Junli Ye Lijun Chai Qiang Xu Zhiyong Pan Shunyuan Xiao Paul D.Fraser Xiuxin Deng | 2019 | Molecular Plant2019，12，9： | 18 |
| 8 | Efficient InGaN-based yellow-light-emitting diodes显示文摘Realization of efficient yellow-light-emitting diodes(LEDs) has always been a challenge in solid-state lighting.Great effort has been made, but only slight advancements have occurred in the past few decades. After comprehensive work on InGaN-based yellow LEDs on Si substrate, we successfully made a breakthrough and pushed the wall-plug efficiency of 565-nm-yellow LEDs to 24.3% at 20 A∕cm^2 and 33.7% at 3 A∕cm^2. The success of yellow LEDs can be credited to the improved material quality and reduced compressive strain of InGaN quantum wells by a prestrained layer and substrate, as well as enhanced hole injection by a 3 D pn junction with V-pits. | FENGYI JIANG JIANLI ZHANG LONGQUAN XU JIE DING GUANGXU WANG XIAOMING WU XIAOLAN WANG CHUNLAN MO ZHIJUE QUAN XING GUO CHANGDA ZHENG SHUAN PAN JUNLIN LIU | 2019 | Photonics Research2019，7，2： | 13 |
| 9 | Layer-by-layer deposition of bioactive layers on magnesium alloy stent materials to improve corrosion resistance and biocompatibility显示文摘Magnesium alloy is considered as one of the ideal cardiovascular stent materials owing to its good mechanical properties and biodegradability.However,the in vivo rapid degradation rate and the insufficient biocompatibility restrict its clinical applications.In this study,the magnesium alloy(AZ31B)was modified by combining the surface chemical treatment and in-situ self-assembly of 16-phosphonyl-hexadecanoic acid,followed by the immobilization of chitosan-functionalized graphene oxide(GOCS).Heparin(Hep)and GOCS were alternatively immobilized on the GOCS-modified surface through layer by layer(LBL)to construct the GOCS/Hep bioactive multilayer coating,and the corrosion resistance and biocompatibility were extensively explored.The results showed that the GOCS/Hep bioactive multilayer coating can endow magnesium alloys with an excellent in vitro corrosion resistance.The GOCS/Hep multilayer coating can significantly reduce the hemolysis rate and the platelet adhesion and activation,resulting in an excellent blood compatibility.In addition,the multilayer coating can not only enhance the adhesion and proliferation of the endothelial cells,but also promote the vascular endothelial growth factor(VEGF)and nitric oxide(NO)expression of the attached endothelial cells on the surfaces.Therefore,the method of the present study can be used to simultaneously control the corrosion resistance and improve the biocompatibility of the magnesium alloys,which is expected to promote the application of magnesium alloys in biomaterials or medical devices,especially cardiovascular stent. | Fan Gao Youdong Hu Guicai Li Sen Liu Li Quan Zhongmei Yang Yanchun Wei Changjiang Pan | 2020 | Bioactive Materials2020，5，3： | 12 |
| 10 | Formation control and collision avoidance for multi-UAV systems based on Voronoi partition显示文摘This paper deals with the formation control problem of multiple unmanned aerial vehicles(UAVs) with collision avoidance. A distributed formation control and collision avoidance method is proposed based on Voronoi partition and conventional artificial potential field. The collision avoidance is achieved by partitioning the whole space into non-overlapping regions based on Voronoi partition theory, which is taken as the task region to confine the movement of each UAV. The general motion control law is designed based on the conventional artificial potential field. As this often leads to local optimum when two UAVs are going to collide with each other and they may stay still where the repulsive force is adversely equivalent to the attractive force. To address this problem,the destination switch scheme is further proposed to let UAVs switch destinations when they reach the local equilibrium. Finally,the effectiveness of proposed formation control algorithm is validated by simulations and experiments. | HU JinWen WANG Man ZHAO ChunHui PAN Quan DU Chang | 2020 | Science China(Technological Sciences)2020，63，1： | 12 |
| 11 | Formability,microstructure evolution and mechanical properties of wire arc additively manufactured AZ80M magnesium alloy using gas tungsten arc welding显示文摘Wire arc additive manufacturing(WAAM)technology has been used to fabricate the multi-layer single-pass deposited wall of AZ80M magnesium(Mg)alloy by gas tungsten arc welding.The formability,thermal cycles,microstructural evolution and mechanical properties of the WAAM AZ80M Mg alloy were investigated.The results show that there was significant difference in the temperature variation and the geometries between the original several layers and the subsequent deposited layers.Owing to the arc energy input,the interpass temperature rised rapidly and then stabilized at 150℃.As a result,the width of the deposited wall increased and then kept stable.There were obvious differences in the microstructure of the WAAM AZ80M Mg alloy among the top zone,intermediate zone and bottom zone of deposited wall.During the arc deposition process,theβphase of the WAAM AZ80M Mg alloy redissolved due to the cyclic heat accumulation,and then precipitated in the grain boundary.The cyclic heat accumulation also led to weakening of dendrite segregation.From the substrate to the top zone,the hardness of the deposited wall decreased gradually,and the intermediate zone which was the main body of deposited wall had relatively uniform hardness.The tensile properties of the WAAM AZ80M Mg alloy were different between the vertical direction and the horizontal direction.And the maximum ultimate tensile strength of the WAAM AZ80M Mg alloy was 308 MPa which was close to that of the as-extruded AZ80M Mg alloy. | Yangyang Guo Gaofeng Quan Yinglong Jiang Lingbao Ren Lingling Fan Houhong Pan | 2021 | Journal of Magnesium and Alloys2021，9，1： | 10 |
| 12 | Origin of the unusually high dibenzothiophene concentrations in Lower Ordovician oils from the Tazhong Uplift,Tarim Basin,China显示文摘The origin of the unusually high dibenzothiophene (DBT) concentrations in Lower Ordovician oils from the Tazhong Uplift,Tarim Basin was studied by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS).The most abundant sulfur compounds in the oils are S 1 species with doublebond equivalent (DBE) values of 1-19 and 11-48 carbon atoms.The range of the number of carbon atoms in the sulfur compounds detected by the FT-ICR MS (S 1 species with DBE=9) is about ten times larger than that for sulfur compounds detected by GC/MS (DBTs).This suggests that FT-ICR MS is a much better approach than GC/MS for characterization of DBTs in crude oils.The abundance of S 1 species with DBE=1-8 decreased with increasing thermal maturity,while the abundance of S 1 species with DBE=9 (primarily DBTs) increased.Therefore,thermal maturity is an important factor in the formation of oils with high DBT concentrations.Unusually high abundances of S 1 species with low DBE values (1-8),which include sulfide,thiophene and benzothiophene,were observed in several oils,especially the TZ83 (O 1) oil with high or very high thermal maturity.Thermochemical sulfate reduction (TSR) was thought to be the reason for the high abundance of these low DBE compounds in deep reservoirs,and thermochemical sulfate reduction could affect the distribution and composition of DBTs in the oils.According to the results of FT-ICR MS analysis,there are no signs that TSR is occurring or has occurred recently for most of the Lower Ordovician oils. | Li Sumei Pang Xiongqi Shi Quan Zhang Baoshou Zhang Haizu Pan Na Zhao Ming | 2011 | Petroleum Science2011，8，4： | 9 |
| 13 | Synergistic immunoreaction of acupuncture-like dissolving microneedles containing thymopentin at acupoints in immune-suppressed rats显示文摘Dissolving microneedles carried drug molecules can effectively penetrate the stratum corneum of skin to improve the transdermal drug delivery. The traditional Chinese medicine acupuncture is based on the needle stimulation at a specific location(acupoint) to generate and transmit biochemical and physiological signals which alter the pathophysiological state of patients. However, the pain associated with conventional acupuncture needles and the requirement of highly trained professionals limit the development of acupuncture in non-Asian countries. The purpose of this study is to investigate whether the dissolving microneedles can be utilized as a self-administered painless replacement for acupuncture and locally released drug molecules can achieve expected therapeutic outcomes. Immunosuppressive rats were treated with acupuncture at Zusanli(ST36) acupoint using microneedles containing thymopentin.The immune functions and psychological mood of the immunosuppressed animals were examined. The proliferation of splenocytes was examined by CCK-8 assay. CD4 and CD8 expression patterns in spleen cells were detected by flow cytometry. The current study showed that use of either microneedles containing thymopentin or conventional acupuncture both resulted in immune cell proliferation, which was confirmed by flow cytometry. Furthermore, either conventional acupuncture or microneedles were able to effectively mitigate the anxiety caused by immune-suppression when applied on the ST36. | Qian Zhang Chuncao Xu Shiqi Lin Huanbin Zhou Gangtao Yao Hu Liu Lili Wang Xin Pan Guilan Quan Chuanbin Wu | 2018 | Acta Pharmaceutica Sinica B2018，8，3： | 9 |
| 14 | Bilayer dissolving microneedle array containing 5-fluorouracil and triamcinolone with biphasic release profile for hypertrophic scar therapy显示文摘Hypertrophic scar(HS)is an undesirable skin abnormality following deep burns or operations.Although intralesional multi-injection with the suspension of triamcinolone acetonide(TA)and 5-fluorouracil(5-Fu)has exhibited great promise to HS treatment in clinical,the difference of metabolic behavior between TA and 5-Fu remarkably compromised the treatment efficacy.Besides,the traditional injection with great pain is highly dependent on the skill of the experts,which results in poor compliance.Herein,a bilayer dissolving microneedle(BMN)containing TA and 5-Fu(TA-5-Fu-BMN)with biphasic release profile was designed for HS therapy.Equipped with several micro-scale needle tips,the BMN could be self-pressed into the HS with uniform drug distribution and less pain.Both in vitro permeation and in vivo HS retention tests revealed that TA and 5-Fu could coexist in the scar tissue for a sufficient time period due to the well-designed biphasic release property.Subsequently,the rabbit ear HS model was established to assess therapeutic efficacy.The histological analysis showed that TA-5-Fu-BMN could significantly reduce abnormal fibroblast proliferation and collagen fiber deposition.It was also found that the value of scar elevation index was ameliorated to a basal level,together with the downregulation of mRNA and protein expression of Collagen I(Col I)and transforming growth factor-β1(TGF-β1)after application of TA-5-Fu-BMN.In conclusion,the BMN with biphasic release profiles could serve as a potential strategy for HS treatment providing both convenient administrations as well as controlled drug release behavior. | Beibei Yang Yating Dong Yifeng Shen Ailin Hou Guilan Quan Xin Pan Chuanbin Wu | 2021 | Bioactive Materials2021，6，8： | 8 |
| 15 | Curcumenol triggered ferroptosis in lung cancer cells via lncRNA H19/miR-19b-3p/FTH1 axis显示文摘Curcumenol,an effective ingredient of Wenyujin,has been reported that exerted its antitumor potential in a few cancer types.However,the effect and molecular mechanism of curcumenol in lung cancer are largely unknown.Here,we found that curcumenol induced cell death and suppressed cell proliferation in lung cancer cells.Next,we demonstrated that ferroptosis was the predominant method that contributed to curcumenol-induced cell death of lung cancer in vitro and vivo for the first time.Subsequently,using RNA sequencing,we found that the long non-coding RNA H19(lncRNA H19)was significantly downregulated in lung cancer cells treated with curcumenol,when compared to untreated controls.Overexpression of lncRNA H19 eliminated the anticancer effect of curcumenol,while lncRNA H19 knockdown promoted ferroptosis induced by curcumenol treatment.Mechanistically,we showed that lncRNA H19 functioned as a competing endogenous RNA to bind to miR-19b-3p,thereby enhanced the transcription activity of its endogenous target,ferritin heavy chain 1(FTH1),a marker of ferroptosis.In conclusion,our data show that the natural product curcumenol exerted its antitumor effects on lung cancer by triggering ferroptosis,and the lncRNA H19/miR-19b-3p/FTH1 axis plays an essential role in curcumenol-induced ferroptotic cell death.Therefore,our findings will hopefully provide a valuable drug for treating lung cancer patients. | Ruonan Zhang Ting Pan Yu Xiang Mingming Zhang Han Xie Zimao Liang Bi Chen Cong Xu Jing Wang Xingxing Huang Qianru Zhu Ziming Zhao Quan Gao Chengyong Wen Wencheng Liu Weirui Ma Jiao Feng Xueni Sun Ting Duan Elaine Lai-Han Leung Tian Xie Qibiao Wu Xinbing Sui | 2022 | Bioactive Materials2022，7，7： | 8 |
| 16 | Simulation of phase transition process using lattice Boltzmann method显示文摘A new lattice Boltzmann model based on SC model, is proposed to describe the liquid-vapor phase transitions. The new model is validated through the simulation of the one-component phase transition process. Compared with the simulation results of van der Waals gas and the Maxwell equal-area construction, the results of the new model have a better agreement with the analytical solutions than those of SC and Zhang models. Since the obtained temperature range and the maximum density ratio in this model are expanded, and the magnitude of maximum spurious current is only between those of SC and Zhang models, it is believed that this new model has better stability than SC and Zhang models. Consequently, the application scope of this new model is expanded compared with the existing phase transition models. According to the principle of the corresponding states in engineering thermodynamics, the simulations of ammonia and water phase transition process were implemented using this new model with different equations of state. Compared with the experimental data of ammonia and water, the results show that the Peng-Robinson is the best equation of state to describe the phase transition process of ammonia and water. Especially, the simulation results of ammonia with Peng-Robinson equation of state have an excellent agreement with its experimental data. Therefore these simulation results have a significant influence on the real engineering applications. | ZENG JianBang LI LongJian LIAO Quan CUI WenZhi CHEN QingHua PAN LiangMing | 2009 | Chinese Science Bulletin2009，54，24： | 8 |
| 17 | Evaluation of Vaporized Hydrogen Peroxide Fumigation as a Method for the Bio-decontamination of the High Efficiency Particulate Air Filter Unit显示文摘Objective To evaluate the performance of vaporized hydrogen peroxide (VHP) for the bio-decontamination of the high efficiency particulate air (HEPA) filter unit. Methods Self-made or commercially available bioindicators were placed at designated locations in the HEPA filter unit under VHP fumigation. The spores on coupons were then extracted by 0.5 h submergence in eluent followed by 200-time violent knocks. Results Due to the presence of HEPA filter in the box, spore recovery from coupons placed at the bottom of the filter downstream was significantly higher than that from coupons placed at the other locations. The gap of decontamination efficiency between the top and the bottom of the filter downstream became narrower with the exposure time extended. The decontamination efficiency of the bottom of the filter downstream only improved gently with the injection rate of H2O2 increased and the decontamination efficiency decreased instead when the injection rate exceeded 2.5 g/min. The commercially available bioindicators were competent to indicate the disinfection efficiency of VHP for the HEPA filter unit. Conclusion The HEPA filter unit is more difficult than common enclosure to decontaminate using VHP fumigation. Complete decontamination can be achieved by extending fumigation time. VHP fumigation can be applied for in-situ biodecontamination of the HEPA filter unit as an alternative method to formaldehyde fumigation. | JIA Hai Quan LI Yan Ju SUN Bei ZHAO Si Qing YI Ying ZHAO Ming ZHANG Zong Xing PAN Xin QI Jian Cheng | 2013 | Biomedical and Environmental Sciences2013，26，2： | 8 |
| 18 | Practical integrated navigation fault detection algorithm based on sequential hypothesis testing显示文摘In detecting system fault algorithms,the false alarm rate and undectect rate generated by residual Chi-square test can affect the stability of filters.The paper proposes a fault detection algorithm based on sequential residual Chi-square test and applies to fault detection of an integrated navigation system.The simulation result shows that the algorithm can accurately detect the fault information of global positioning system(GPS),eliminate the influence of false alarm and missed detection on filter,and enhance fault tolerance of integrated navigation systems. | Feng Yang Cheng Cheng Quan Pan Gongyuan Zhang | 2011 | Journal of Systems Engineering and Electronics2011，22，1： | 7 |
| 19 | Development of composite PLGA microspheres containing exenatide-encapsulated lecithin nanoparticles for sustained drug release显示文摘This study aimed to prepare poly(D, L-lactic-co-glycolic acid) microspheres(PLGA-Ms)by a modified solid-in-oil-in-water(S/O/W) multi-emulsion technique in order to achieve sustained release with reduced initial burst and maintain efficient drug concentration for a prolonged period of time. Composite PLGA microspheres containing exenatideencapsulated lecithin nanoparticles(Ex-NPs-PLGA-Ms) were obtained by initial fabrication of exenatide-loaded lecithin nanoparticles(Ex-NPs) via the alcohol injection method,followed by encapsulation of Ex-NPs into PLGA microspheres. Compared to Ms prepared by the conventional water-in-oil-in-water(W/O/W) technique(Ex-PLGA-Ms), Ex-NPs-PLGAMs showed a more uniform particle size distribution, reduced initial burst release, and sustained release for over 60 d in vitro. Cytotoxicity studies showed that Ms prepared by both techniques had superior biocompatibility without causing any detectable cytotoxicity.In pharmacokinetic studies, the effective drug concentration was maintained for over 30 d following a single subcutaneous injection of two types of Ms formulation in rats, potentially prolonging the therapeutic action of Ex. In addition, administration of Ex-NPs-PLGA-Ms resulted in a more smooth plasma concentration-time profile with a higher area under the curve(AUC) compared to that of Ex-PLGA-Ms. Overall, Ex-NPs-PLGA-Ms prepared by the novel S/O/W method could be a promising sustained drug release system with reduced initial burst release and prolonged therapeutic efficacy. | Ni Dong Chune Zhu Junhuang Jiang Di Huang Xing Li Guilan Quan Yang Liu Wen Tan Xin Pan Chuanbin Wu | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，3： | 7 |
| 20 | Expression of Beclin1 in non small cell lung cancer and its clinical significance显示文摘Objective:To investigate the expression of autophagy related gene;Beclin1 in human non-small cell lung cancer(NSCLC)tissues.Methods:Protein expression of Beclin1 was determined by immunofluorescence staining and Western Blot,mRNA expression was analyzed by RT-PCR.Results:Immunofluorescence staining revealed that the level of Beclin1 expression in lung cancer was significantly lower than that in adjacent noncancerous tissues and normal tissues(expression rate 8.3%,P=0.000).The Beclin1 mRNA expression in lung cancer,adjacent noncancerous tissues and normal tissues was 1.372(±0.475)1.721(±0.521)and 1.553(±0.554)when F = 15.0,P < 0.01.Beclin1 protein expression in lung cancer,adjacent noncancerous tissues and normal tissues was 3.453(±0.852)5.423(±1.351)and 6.878(±0.997)F = 11.2,P < 0.01.Conclusion:The protein and mRNA expression of Beclin1 in lung cancer was much lower than those in and around cancer tissues and normal tissues,those differences having statistical significance.However in adjacent noncancerous tissues and normal tissues,the expression showed no difference. | Quan Liu Jianjun Wang Yongcheng Pan Lifeng Meng Xi Zhan | 2008 | Journal of Nanjing Medical University2008，22，2： | 6 |
您所在的机构暂未开通此部分的功能，请联系管理员