|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Innovative immunohistochemistry identifies MMP-9 expressing macrophages at the invasive front of murine HCC显示文摘AIM:To investigate the proteolytic contribution of tumor-associated macrophages(TAM)in tumor invasion,we analyzed whether TAM at the invasive front of small HCC in Abcb4-/--mice show an enhanced expression of MMP-9. METHODS:Liver cryosections of the hepatocellular carcinoma(HCC)invasive front from 12 mo old Abcb4-/--mice were stained for collagen typeⅠand MMP-9 using Alexa488 and Alexa568 labeled secondary antibodies.Afterwards,the Alexa568 dye was bleached and the macrophage marker F4/80 was visualized using Alexa568 labeled secondary antibodies.Finally, photographs of the invasive tumor front were digitally overlaid and analyzed. RESULTS:After complete bleaching of the primary dye,specific fluorescence staining of a third antigen, here F4/80,was successfully performed on the same histological section.With this method,we were able to identify conglomerates of matrix metalloproteinase (MMP-9)expressing macrophages within the tumor capsule of HCC. CONCLUSION:MMP-9 expressing macrophages are involved in matrix remodelling at the invasive tumor front of HCC.The described staining protocol provides a simple yet powerful extension of conventional immuno-histochemistry,facilitating visualization of at least three different antigens plus nuclei in one single histological section. | Martin Roderfeld Timo Rath Frank Lammert Christian Dierkes Jürgen Graf Elke Roeb | 2010 | World Journal of Hepatology2010，2，5： | 6 |
| 2 | Calcitriol analog ZK191784 ameliorates acute and chronic dextran sodium sulfate-induced colitis by modulation of intestinal dendritic cell numbers and phenotype显示文摘AIM: To investigate the effects of ZK1916784, a low calcemic analog of calcitriol on intestinal inflammation. METHODS: Acute and chronic colitis was induced by dextran sodium sulfate (DSS) according to standard procedures. Mice were treated intraperitoneally with ZK1916784 or placebo and colonic inflammation was evaluated. Cytokine production by mesenterial lymph node (MLN) cells was measured by ELISA. Immunohistochemistry was performed to detect intestinal dendritic cells (DCs) within the colonic tissue, and the effect of the calcitriol analog on DCs was investigated. RESULTS: Treatment with ZK191784 resulted in significant amelioration of disease with a reduced histological score in acute and chronic intestinal inflammation. In animals with acute DSS colitis, down- regulation of colonic inflammation was associated with a dramatic reduction in the secretion of the proinflammatory cytokine interferon (IFN)-γ and a significant increase in intereleukin (IL)-10 by MLN cells. Similarly, in chronic colitis, IL-10 expression in colonic tissue increased 1.4-fold when mice were treated with ZK191784, whereas expression of the Th1-specific transcription factor T-beta decreased by 81.6%. Lower numbers of infiltrating activated CD11c+ DCs were found in the colon in ZK191784-treated mice with acute DSScolitis, and secretion of proinflammatory cytokines by primary mucosal DCs was inhibited in the presence of the calcitriol analog. CONCLUSION: The calcitriol analog ZK191784 demonstrated significant anti-inflammatory properties in experimental colitis that were at least partially mediated by the immunosuppressive effects of the derivate on mucosal DCs. | Ulrike G Strauch Florian Obermeier Nicole Grunwald Nadja Dunger Heiko C Rath Jürgen Schlmerich A Steinmeyer U Zügel HH Herfarth | 2007 | World Journal of Gastroenterology2007，13，48： | 4 |
| 3 | 高速水冷淬火过程中残余压应力的形成显示文摘据文献报道,快速水冷淬火可在试样表面形成残余压应力(RSs)从而显著提高其疲劳极限,这种技术被称为'强烈淬火'。德国热处理协会'淬火'技术委员会发起了一个旨在精确研究上述问题的研究项目。本文重点介绍马氏体相变对残余应力形成的影响。为了在圆柱试样表面得到残余压应力,需要确定合适的工艺窗口。在此窗口中,淬火冷却速度要求足够快,以致于在圆柱体内达到最大温度梯度之前,表面温度已经低于Ms。 | J Rath T Liibben F Hoffmann H-W Zoch 顾剑锋 | 2013 | 热处理2013，28，2： | 3 |
| 4 | Matrix metalloproteinase-13 is regulated by toll-like receptor-9 incolorectal cancer cells and mediates cellular migration显示文摘 | Timo Rath Julia St?ckle Martin Roderfeld Annette Tschuschner Jürgen Graf Elke Roeb | 2011 | Oncology Letters2011，，3： | 2 |
| 5 | Matrix metalloproteinases in inflammatory bowel disease-from basic research to clinical significance显示文摘 | Rath T Roderfeld M Graf J | 2009 | ZGastroenterol2009，47，8： | 1 |
| 6 | Jejuno-colonic shunt in the treatment of extreme obesity显示文摘 | Rath R Buda J Bittnerova H | 1968 | Cesk Gastroenterol Vyz1968，22，5： | 1 |
| 7 | Structure and function of the eukaryotic ribosome:the next frontier 显示文摘 | Doudna J A Rath V L | 2002 | Cell2002，109，2： | 1 |
| 8 | Preparation of ti- tanium metal by electrochemical reduction of titanium dioxide显示文摘 | Mohanty J Rath P C Subbaiah T | 2009 | Transactions of the Indian Institute of Metals2009，62，3： | 1 |
| 9 | Role of caveolar compartmentation in endothelium-derived hyperpolarizing factor-mediated relaxation:Ca2+ signals and gap junction function are regulated by caveolin in endothelial cells显示文摘 | Saliez J Bouzin C Rath G | | 0，，08： | 1 |
| 10 | Heat of wood pyrolysis显示文摘 | J Rath Wolfinger M G Steiner G | 2003 | Fuel2003，82，1： | 1 |
| 11 | Effects of enamel matrix derivative on periodontal wound healing in an inflammatory environ- ment in vitro显示文摘 | ] Nokhbehsaim M Winter J Rath B el al | 2011 | J Clin Periodontol2011，38，5： | 1 |
| 12 | Fluorescent copper(I)complexes:an X-ray diffraction study of complexes of copper(I)iodide and pyridine derivatives of rhombic, ,and polymeric structure, and 显示文摘 | Rath N P Maxwell J L Holt E M | 1986 | J Chem Soc Dalt-on Trans1986，11，： | 1 |
| 13 | Matrix metalloprotei- nase inflammatory bowel disease from basic research to clinical sig- nify cance显示文摘 | RATH T RODERFELD M GRAF J | 2009 | Z Gastroenterol2009，47，8： | 1 |
| 14 | 1 Matrixmetallop roteinases in inflamm a- tory bowel disease-from basic research to clinical significance显示文摘 | Rath T Roderfeld M Graf J et a | 2009 | Zgas- troen tero2009，47，8： | 1 |
| 15 | Compressive forces in- duce osteogenie gene expression in ealvarial osteoblasts显示文摘 | Rath B Nam J Knobloeh TJ | 2008 | J Biomech2008，41，5： | 1 |
| 16 | Expression profile of matrix metalloproteinase-2 and -9 and their endogenous tissue inhibitors in osteonecrotic femoral heads显示文摘 | Gr(o)ssel S Beckmann J Rath B | 2010 | Int J Mol Med2010，26，1： | 1 |
| 17 | Thin-film transistors deposited by hot-wire chemical vapor deposition 显示文摘 | Stannowski B Rath J K Schropp R E I | 2003 | Thin Solid Films2003，430，12： | 1 |
| 18 | Effect of intermittent activity on broiler production parameters 显示文摘 | Balog J M G R Bayyari N C Rath | 1997 | Poultry Science1997，76，： | 1 |
| 19 | Incorporation of p-type Microcrystalline Silicon Films in Amorphous Silicon Based Solar Cells in a Superstrate Structure 显示文摘 | Rath J K Schropp R E I | 1998 | Solar Energy Materials and Solar Cells1998，53，： | 1 |
| 20 | Hydrogenated amorphous and polycrystalline silicon TFTs by hotwire CVD显示文摘 | RATH J K BARBON A SCHROPP R E I | 1998 | J Non-Cryst Solids1998，，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员