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| 1 | 大豆分枝数相关分子标记开发及qBN-18位点精细定位显示文摘分枝数是影响大豆产量的重要因素之一,与植株结荚率直接相关;同时也是决定大豆株型的重要组成因子,并通过调节群体结构、种植密度等进一步影响产量。目前关于大豆分枝数QTL(quantitative trait loci)精细定位与图位克隆的报道极少。因此,发掘参与调控大豆分枝的基因/QTL对于株型建成的基础研究和高产品种培育的应用研究都具有重要意义。本研究在少分枝品种垦丰19(KF19)与多分枝品种垦农24(KN24)组合F2的基础上,培育出由606个株系组成的F7:8重组自交系(recombinant inbred lines,RIL)群体,以及由1486个单株KF19-BC3F2和1150个单株KN24-BC2F2组成的2个回交群体。在18号染色体分枝数QTL新位点(qBN-18)的定位区间内筛选出多态性SSR标记11个,利用RIL群体将qBN-18的定位区间由1.6 Mb缩小到113 kb。在定位区间内开发了2个InDel标记BR69与BR77。进一步利用回交群体筛选交换单株,将qBN-18定位区间缩小到63.7 kb,包括9个基因。本研究结果为大豆分枝数的基因图位克隆及分子标记辅助育种创造了条件。 | 吴海涛 张勇 苏伯鸿 Lamlom F Sobhi 邱丽娟 | 2020 | 作物学报2020，46，11： | 4 |
| 2 | KIR and a specific HLA-C gene are associated with susceptibility and resistance to hepatitis B virus infection in a Brazilian population显示文摘 | Patricia Araujo Giovana Goncalves Flavia Latini Orlando Ferreira Luis Cristovao Porto Jose Augusto Barreto Manoel Joao Castelo Girao Ricardo Sobhie Diaz | 2014 | Cellular & Molecular Immunology2014，11，6： | 3 |
| 3 | Map-based cloning of a novel QTL qBN-1 influencing branch number in soybean[Glycine max(L.)Merr.]显示文摘Branch number(BN)is an important agronomic attribute related to the plant architecture,adaptability,and yield of soybean.To date,few studies ofBNhave been conducted to elucidate its genetic background.We aimed to localize genetic factors affecting BN using segregating populations derived fromthe high-branching cultivar‘Kennong24’(KN24)and the low-branching cultivar‘Kenfeng19’(KF19).Composite interval mapping analysis detected a QTL(qBN-1)on chromosome 6 between the SSR markers BARCSOYSSR_06_0993 and BARCSOYSSR_06_1070 using an F2 population.To fine-map qBN-1,a RIL population was developed and genotyped with 14 SSRmarkers located in the QTL region.qBN-1 was localized to a 115.67-kb interval flanked by markers BARCSOYSSR_06_1048 and BARCSOYSSR_06_1053.The QTL was further confirmed using backcross populations of size 1305(BC2F2 with KN24 as a recurrent parent)and 1712(BC3F2 with KF19 as a recurrent parent).The fine-mapping region of qBN-1 contained only two candidate genes,Glyma.06G208800 and Glyma.06G208900,whose expression patterns were investigated by qRT-PCR.Compared to Glyma.06G208800 gene expression,Glyma.06G208900 showed the highest expression of the two genes and showed a significant difference in expression between high-and low-branching genotypes in either axillary meristem or shoot apical meristem,and showed opposite expression patterns in the two tissues at V4 and R1 stages.These results identify Glyma.06G208900 as a novel candidate gene controlling BN.Taken together,the results of this study provide a foundation for cloning and functional analysis of the qBN-1 gene and for the improvement of BN bymarker-assisted selection in soybean breeding. | Sobhi F.Lamlom Yong Zhang Bohong Su Haitao Wu Xia Zhang Jindong Fu Bo Zhang Li-Juan Qiu | 2020 | The Crop Journal2020，8，5： | 2 |
| 4 | Involvement of Quebracho tannins in diet alters productive and reproductive efficiency of postpartum buffalo cows显示文摘This study was conducted to investigate the effects of 10 weeks supplementation of Quebracho tannins(QT; 0 [control], 100 [QT100] or 200 g/[cow$d] [QT_(200)]) to 30 multiparous postpartum buffalo cows(10 cows per group) on milk yield and composition, blood metabolites and reproductive performance.Supplementation of QT100 had no significant effect on milk yield, whereas QT_(200) decreased(P < 0.05) this trait. Compared with the control group, both QT levels decreased(P < 0.05) fat-corrected milk(FCM)yield, but no significant effects were found on percentages of milk fat and protein. Contrariwise, yields of milk fat, lactose and milk protein were decreased(P < 0.05) when QT_(200) was supplemented. The solids nonfat(SNF) percentage and yield were decreased(P < 0.05) with QT100 supplementation. Moreover, QT tended to numerically reduce total number of ovarian follicles, number of small follicles, peripheral progesterone concentration and conception rate. Supplementation of QT_(200) numerically increased number of large follicles, mean diameter of large follicle, number and diameters of corpora lutea. The inclusion of QT_(200) shortened days open(DO) and decreased number of services per conception.Contrariwise, QT did not show significant effects on serum total protein, albumin, globulin, glucose,cholesterol and triglycerides concentrations. Supplementation of QT100 caused an increase(P < 0.05) of serum urea compared with that in control and QT_(200) groups. Generally, QT decreased(P < 0.05) serum creatinine concentration. Therefore, the supplementation of a commercial QT to early lactating Egyptian buffalo cows displayed negative consequences on their productive and reproductive performances. | Sobhy M.A.Sallam Marwa F.A.Attia Adel N.M.Nour El-Din Samir Z.El-Zarkouny Adel M.Saber Hani M.El-Zaiat Moustafa M.Zeitoun | 2019 | Animal Nutrition2019，，1： | 2 |
| 5 | Postrepair fatigue performance of FRP-repaired corroded RC beams: experimental and analytical investigation 显示文摘 | Masoud Sobhy Soudki Khaled Topper Timothy | 2005 | Journal of Composites for Construction2005，9，5： | 1 |
| 6 | Numerical investigation of the stresses in backfilled stopes overlying a sill mat显示文摘Backfill is commonly used in underground mines to help increase the ore recovery rate and reduce the ore dilution. The use of a part of mine waste as underground backfill material also helps reduce the environmental impact of mining operations. After all, backfill is used to provide a working platform or safer working space. Its primary and most important role is to improve the rock mass stability around mine openings. However, most available solutions to stress analyses were developed for an isolated stope, without taking into account the influence of mine depth, or of adjacent stopes. In this paper,results from a numerical study carried out to evaluate the stresses in backfilled stopes overlying a sill mat are presented. Mine depth and excavation of the underlying stope below the sill mat(horizontal pillar)are both taken into consideration. The influence of stope geometry, backfill, sill mat and rock properties on the stresses is also evaluated. Compared with the case of a single isolated backfilled stope, the numerical results show that the stress magnitudes in the overlying backfill are considerably increased due to the excavation of the underlying stope. In general, the stresses also increase with mine depth and backfill stiffness, while these tend to decrease with an increase in the surrounding rock mass stiffness.These results suggest that existing solutions for backfill design may need to be revised. | Mohamed Amine Sobhi Li Li | 2017 | Journal of Rock Mechanics and Geotechnical Engineering2017，9，3： | 1 |
| 7 | Using trichloroacetic acid in the treatment of acanthosis nigricans : a pilot study 显示文摘 | Zayed A Sobhi RM Abdel Halim DM | 2014 | J Derma- tolog Treat2014，25，3： | 1 |
| 8 | Boundary conditions for general ordinary differential operators and their adjoints 显示文摘 | Evans W D Sobhy E I | 1990 | Proceedings of the Royal Society of Edinburgh1990，114，： | 1 |
| 9 | Penetration depth of nickel titanium and stainless steel finger spreaders in curved root canals显示文摘 | Sobhi MB Khan I | 2003 | Coll Physicians Surg Pak2003，13，2： | 1 |
| 10 | De-creased axillary lymph node retrieval in patients after neoadjuvantchemotherapy显示文摘 | BASLAIM M M AL MALIK 0 A AL SOBHI S S | 2002 | Am J Surg2002，184，4： | 1 |
| 11 | Dry cleaning of pulverizedcoal using a novel rotary triboelectrostatic separator(RTS) 显示文摘 | Tao D Sobhy A Li Q | 2011 | International Journal of Coal Preparation andUtilization2011，，31： | 1 |
| 12 | Postrepair fatigue performance of FRP-repaired corroded RC beams: Experimental and analytical investigation 显示文摘 | Masoud Sobhy Soudki Khaled Topper Timothy | 2005 | Journal of Composites for Construction2005，9，5： | 1 |
| 13 | An interface pullout formula for extensible sheet reinforcement显示文摘 | SOBHI S WU J T H | 1996 | Geosynthetics International1996，3，5： | 1 |
| 14 | Effect of vitamin B12 on chloramphenicol and thiamphenicol used on DNA, with reference to cytogenetic biochemical analysis and pathological studies in rats显示文摘 | Sobhy H M Mohamed E A Shehab G G | 2000 | Egyptian J-Agricult Res2000，78，1： | 1 |
| 15 | Community-acquired methicillin-resistant Staphylococcus aureus from skin and soft tissue infections (in a sample of Egyptian population) analy- sis of mec gene and staphylococcal cassette chromosome显示文摘 | Sobhy N Aly F Abd E1 Kader O | 2012 | Braz J Iufect Dis2012，16，5： | 1 |
| 16 | Neurovascular safety and clinical outcome of outside-in repair of tears of the pos- terior horn of the medial meniscus 显示文摘 | Sobhy M H AbouEloud M M Kamel E M | 2010 | Arthroscopy2010，26，12： | 1 |
| 17 | Naphthalene combustion in supercritical water flames显示文摘 | Sobhy A Guthrie R I L Butler I S | 2009 | Proceedings of the Combustion Institute2009，32，： | 1 |
| 18 | Methods of Attacking Chaotic Encryption and Countermeasures显示文摘 | ALAA-ELDIN R SHEHATA | 2001 | IEEE International Conference Acoustics Speech and Signal Processing2001，2，： | 1 |
| 19 | The transition from solitons to chaos in the solution of the Logistic equation显示文摘 | Sobhy M I | 2000 | Int J of Bifurcation and Chaos2000，10，10： | 1 |
| 20 | Vascular endothelial growth factor expression is higher in differentiated thyroid cancer than in normal or benign thyroid显示文摘 | Soh EY Duh QY Sobhi SA | 1997 | J ClinEndocrinol Metab1997，82，11： | 1 |
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