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| 1 | Claudin-11 and occludin are major contributors to Sertoli cell tight junction function, in vitro显示文摘Sertoli 房间紧密的连接(TJ ) 是血睾丸障碍的关键部件,在它扣押开发在生精的小管以内经历精子发生的细菌房间的地方。神经质地调整的 claudin-11 是在不孕涉及障碍功能和它的鼠科的猛烈结果的批评 transmembrane 蛋白质。我们试图估计份量上到 TJ 功能的 claudin-11 的贡献的意义在 vitro,使用调停 siRNA 的基因 silencing。我们也进行了 occludin 的贡献的分析, TJ 的另一内在的 transmembrane 蛋白质。claudin-11 或 occludin 的 Silencing 在一个不成熟的老鼠 Sertoli 房间文化模型用 siRNA 被进行。Transepithelial 电的抵抗被用来在整个文化估计份量上 TJ 功能。在 siRNA 处理以后的二天,房间为对 mRNA 的 RT-PCR 评价为连接蛋白质或 lyzed 的 immunocytochemical 本地化被修理表示。claudin-11, occludin,或两个的 Silencing 在 55% 的 TJ 功能导致了重要减少(P <0.01 ) , 51%(P <0.01 ) ,并且 62%(P <0.01 ) 分别地。数据在到 Sertoli 房间 TJ 的指向的蛋白质的本地化是有在 mRNA 表示和显著减小的重要减少的伴随物。我们提供 claudin-11 显著地贡献的量的证据(P <0.01 ) 到 Sertoli 房间 TJ 在 vitro 工作。有趣地, occludin,神经质地在不孕被调整然而并非含有直到迟了的成人生活,也是一重要(P <0.01 ) 贡献者到障碍功能。我们的数据与一致在清楚地为这些表明一个角色的 vivo 研究,在维持正常 TJ 障碍的蛋白质组织并且工作。 | Mark J McCabe Caroline FH Foo Marcel E Dinger Peter M Smooker Peter G Stanton | 2016 | Asian Journal of Andrology2016，18，4： | 7 |
| 2 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 3 | Effects of lactated Ringer's solutions on human leukocytes显示文摘 | Koustova E Stanton K Gushchin V | 2002 | J Trauma2002，52，5： | 1 |
| 4 | Emergy evaluation of the performance and sustainability of three agricultural systems with different scales and management显示文摘 | Martin J F Diemont S A W Powell E Stanton M Levy-Tacher S | 2006 | Agriculture Ecosystems&Environment2006，115，14： | 1 |
| 5 | Test for the presence of sulfate-reducing bacteria显示文摘 | TATNALL R E STANTON K M EBERSOLE R C | 1988 | General interest1988，27，8： | 1 |
| 6 | Heterologous expression of lactose-and galactose-utilizing pathways from lactic acid bacteria in Coryaebocterium glutamicum for pro duction of lysine in whey 显示文摘 | BARRETT E STANTON C ZELDER O | 2004 | Applied and Environmental Mi crobiology2004，70，5： | 1 |
| 7 | Coping Resources, Coping Processes, and Mental Health显示文摘 | Taylor S E Stanton A L | 2007 | Annual Review of Clini- cal Psychology2007，3，1： | 1 |
| 8 | Superiority of ambulatory over clinic blood pressure measurement in predicting mortality: the Dublin outcome study显示文摘 | Dolan E Stanton A Thijs L | 2005 | Hypertension2005，46，1： | 1 |
| 9 | Eyeblink classical conditioning in the preweanling lamb显示文摘 | Johnson TB Stanton M E Goodlett C R | 2008 | BehavNeurosci2008，122，3： | 1 |
| 10 | Periurethral collagen injection:a long-term follow- up study显示文摘 | Gorton E Stanton S Monga A | 1999 | BJU Int1999，84，9： | 1 |
| 11 | Superiority of ambulatory over clinic blood pressure measurement in predicting mortality:the Dublin outcome study显示文摘 | Dolan E Stanton A Thijs L | 2005 | Hypertension2005，46，： | 1 |
| 12 | Superiority of ambulatory over clinic blood pressuremeasurement in predictingmortality: the Dublin outcome study显示文摘 | Dolan E Stanton A Thijs L | 2005 | Hypertension2005，46，1： | 1 |
| 13 | Ambulatory blood pressure monitoring predicts cardiovascular events in treated hypertensive patients-an Anglo-Scandinavian cardiac outcomes trial substudy显示文摘 | Dolan E Stanton AV Thom S | 2009 | J Hypertens2009，27，4： | 1 |
| 14 | Superiority of ambulatory over clinic blood pressure measurement in predicting mortality~ the Dublin outcome study显示文摘 | Dolan E Stanton A Thijs L | 2005 | Hypertension2005，46，1： | 1 |
| 15 | Effects of lactated Ringer's solutions on human leukocytes显示文摘 | Koustova E Stanton K Gushchin V | 2002 | J Trauma2002，52，5： | 1 |
| 16 | The impact on coronary artery disease of common polymorphisms known to modulate responses to pathogens 显示文摘 | O Halloran AM Stanton A O: Brien E | 2006 | Ann Hum Genet2006，70，6： | 1 |
| 17 | Cardiac troponin I, a possible predictor of survival in patients with stable con- gestive heart failurel显示文摘 | Stanton E B Hansen M S Sole M J | 2005 | Can J Cardiol2005，21，1： | 1 |
| 18 | The surgical management of fibrous dysplasia of bone 显示文摘 | Stanton RP Ippolito E Springfield D | 2012 | Orphanet J Rare Dis2012，7，1： | 1 |
| 19 | Human lymphatic pumping measured in healthy and lymphoedematous arms by lymphatic congestion lymphoscintigraphy显示文摘 | S.Modi A.W B Stanton W E Svensson | | 0，，12： | 1 |
| 20 | Application of phasor measurements and partial energy analysis in stabilizing large disturbances显示文摘 | STANTON S E | 1995 | IEEE Transactions on Power Systems1995，10，1： | 1 |
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