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| 1 | Intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease显示文摘AIM:To investigate intestinal alkaline phosphatase(iAP) in the intestinal mucosa of children with inflammatory bowel disease(IBD).METHODS:Colonic biopsy samples were taken from 15 newly diagnosed IBD patients and from 10 healthy controls.In IBD patients,specimens were obtainedboth from inflamed and non-inflamed areas.The iAP mRNA and protein expression was determined by reverse transcription-polymerase chain reaction and Western blotting analysis,respectively.Tissue localization of iAP and Toll-like receptor(TLR) 4 was investigated by immunofluorescent staining.RESULTS:The iAP protein level in the inflamed mucosa of children with Crohn's disease(CD) and ulcerative colitis(UC) was significantly decreased when compared with controls(both P < 0.05).Similarly,we found a significantly decreased level of iAP protein in the inflamed mucosa in CD compared with non-inflamed mucosa in CD(P < 0.05).In addition,the iAP protein level in inflamed colonic mucosa in patients with UC was decreased compared with non-inflamed mucosa in patients with CD(P < 0.05).iAP protein levels in the non-inflamed mucosa of patients with CD were similar to controls.iAP mRNA expression in inflamed colonic mucosa of children with CD and UC was not significantly different from that in non-inflamed colonic mucosa with CD.Expression of iAP mRNA in patients with noninflamed mucosa and in controls were similar.Co-localization of iAP with TLR4 showed intense staining with a dotted-like pattern.iAP was present in the inflamed and non-inflamed mucosa of patients with CD,UC,and in control biopsy specimens,irrespective of whether it was present in the terminal ileum or in the colon.However,the fluorescent signal of TLR4 was more pronounced in the colon compared with the terminal ileum in all groups studied.CONCLUSION:Lower than normal iAP protein levels in inflamed mucosa of IBD patients may indicate a role for iAP in inflammatory lesions in IBD.Based on our results,administration of exogenous iAP enzyme to patients with the active form of IBD may be a therapeutic option. | Kriszta Molnár dám Vannay Beáta Szebeni Nóra Fanni Bánki Erna Sziksz ron Cseh Hajnalka Gyrffy Péter László Lakatos Mária Papp András Arató Gábor Veres | 2012 | World Journal of Gastroenterology2012，18，25： | 5 |
| 2 | Effects of rhamnolipid biosurfactant JBR425 and synthetic surfactant Surfynol465 on the peroxidase-catalyzed oxidation of 2-naphthol显示文摘The kinetics of the recombinant Coprinus cinereus peroxidase-catalyzed2-naphthol oxidationwas investigated in the presence of rhamnolipid biosurfactant JBR425 and synthetic surfactant Surfynol465 at pH 5.5 and25°C,with concentrations of (bio)surfactants both less than critical micelle concentrations (CMC) and larger than CMC. Itwas shown that monomers of JBR425 aswell as monomers of Surfynol465 had an enhancing effect on the conversion of2-naphthol indose response manner anddid not influence the initial rate of 2-naphthol oxidation. The resultswere accounted by a scheme,which contains a stadium of enzyme inhibition by oligomeric2- naphthol oxidation products. The action of the biosurfactant's (or synthetic surfactant's) monomerswas explained by avoidance of the enzyme active center clothingwith oligomers. Similar results havedemonstrated the potential of rhamnolipid biosurfactant JBR425due to its biodegradability. When biosurfactants' concentrations are larger than CMC, (bio)surfactants have an opposite effect on the oxidation of2-naphthol by peroxidase. | Ivanec-Goranina Rūta Kulys Juozas | 2013 | Journal of Environmental Sciences2013，25，7： | 2 |
| 3 | Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy显示文摘AIM:To study the effect of mechanical stress on the cytoskeleton in lens epithelial cells following conventional phacoemulsification surgery(CPS)and femtosecond laserassisted cataract surgery(FLACS).METHODS:The cytoskeleton of the epithelial cells of the anterior lens capsules(ALC)removed by CPS and FLACS was examined by immunohistochemistry.Expression of the intermediate filament,glial fibrillary acidic protein(GFAP),and glutamine synthetase(GS)immunoreactivity were detected.In order to map the actin network of cells,fluorescently labeled phalloidin was used.The samples were examined using confocal laser scanning microscopy.RESULTS:GFAP expression was visible in a larger number of the epithelial cells after CPS compared to FLACS.In CPS sample’s epithelial cells,GFAP immunoreactivity indicated robust morphological change.Regarding the actin filaments,the presence of tubular elements connecting epithelial cells,regular actin pattern and marked cortical network after CPS were found.Following FLACS,the actin cytoskeleton of the epithelial cells remained densely structured,and the tubular elements were undetectable,however,the above-mentioned regular actin pattern and the marked cortical network were visible.CONCLUSION:The conventional removal of the ALC induces more robust changes of the cytoskeleton of the lens epithelial cells. | ANDrea Krisztina Sükosd Krisztina Szabadfi Edina Szabó-Meleg Beáta Gáspár Pavel Stodulka Gyorgy SétálóJr Róbert Gábriel Miklós Nyitrai Zsolt Biró Hajnalka Abrahám | 2020 | International Journal of Ophthalmology(English edition)2020，13，6： | 2 |
| 4 | Involvement of serum retinoids and Leiden mutation in patients with esophageal, gastric, liver, pancreatic, and colorectal cancers in Hungary显示文摘AIM: To analyze the serum levels of retinoids and Leiden mutation in patients with esophageal, gastric, liver,pancreatic, and colorectal cancers.METHODS: The changes in serum levels of retinoids (vitamin A, α- and β-carotene, α- and β-cryptoxanthin,zeaxanthin, lutein) and Leiden mutation were measured by high liquid performance chromatography (HPLC)and polymerase chain reaction (PCR) in 107 patients (70 males/37 females) with esophageal (0/8), gastric (16/5), liver (8/7), pancreatic (6/4), and colorectal (30/21including 9 patients suffering from in situ colon cancer)cancer. Fifty-seven healthy subjects (in matched groups)for controls of serum retinoids and 600 healthy blood donors for Leiden mutation were used.RESULTS: The serum levels of vitamin A and zeaxanthin were decreased significantly in all groups of patients with gastrointestinal (GI) tumors except for vitamin A in patients with pancreatic cancer. No changes were obtained in the serum levels of α- and β-carotene,α- and β-cryptoxanthin, zeaxanthin, lutein in patients with GI cancer. The prevalence of Leiden mutation significantly increased in all groups of patients with GI cancer.CONCLUSION: Retinoids (as environmental factors)are decreased significantly with increased prevalence of Leiden mutation (as a genetic factor) in patients before the clinical manifestation of histologically different (planocellular and hepatocellular carcinoma, and adenocarcinoma) GI cancer. | Gyula Mózsik Gy(o|¨)rgy Rumi András D(o|¨)m(o|¨)t(o|¨)r Mária Figler Beáta Gasztonyi El(?)d Papp Alajos Pár Gabriella Pár József Belágyi Zoltán Matus Béla Melegh | 2005 | World Journal of Gastroenterology2005，11，48： | 2 |
| 5 | The significance of natural and synthetic auxins in the control of stomatal movements 显示文摘 | Cox R C Snaith P J Mansfield TA | 1985 | Acta Horticulture1985，171，： | 1 |
| 6 | Blood pressure and the progression of car - otid atherosclerosis in middle aged men 显示文摘 | Lakka TA Salonen R Kaplan GA | 1999 | Hypertension1999，34，1： | 1 |
| 7 | Relation of sputum inflammatory markers to symptoms and lung function changes in COPD exacerbations 显示文摘 | Bhowmik A Seemungal TA Sapsford R J | 2000 | Thorax2000，55，2： | 1 |
| 8 | Systolic and diastolic function with alternate and combined site pacing in the right ventricle 显示文摘 | Buckingham TA Candinas R | 1998 | PACE1998，21，5： | 1 |
| 9 | Ribotyping in the detec- tion of Clostridium diffieile outbreaks in a single university hospital显示文摘 | Patel TA Smith R Hopkins S | 2012 | J Hosp Infect2012，83，1： | 1 |
| 10 | Calcium anidotrihydroborate:a hydrogen storage material显示文摘 | Diyabalanage H V K Shrestha R P Semelsberger TA Scott B L Bowden M E Davis B L Burrell A K | | 0，，47： | 1 |
| 11 | Amino acid and albumin losses during hemodialysis显示文摘 | Ikizler TA Flakoll P Parker R Hakim R | 1994 | Kidney Int1994，46，： | 1 |
| 12 | 显示文摘 | Elhadd TA Neary R | 1999 | J Clin Endocrinol Metab1999，84，3： | 1 |
| 13 | Local endostatin treatm ent of gliomas administer by microencapsulated producer cell显示文摘 | Read TA Sorensen DR Mahesparan R | 2001 | Nat Biotechno l2001，19，1： | 1 |
| 14 | Local endostatin treatment of giomas administered by microencap-sulated producer cells显示文摘 | Read TA Sorensen DR Mashesparan R | 2001 | Nat Biotchnol2001，19，1： | 1 |
| 15 | A single percu- taneous injection of recombinant human bone morphogenetic protein- 2 accelerates fracture repair显示文摘 | Einhorn TA Majeska R Mohaideen A | 2003 | J Bone Joint Surg (Am)2003，85，8： | 1 |
| 16 | Nanoscale as semblies and their biomedical applications显示文摘 | Doll TA Raman S Dey R | 2013 | J R Soe Interface2013，0，80： | 1 |
| 17 | Acute ischemic stroke in the setting of cervical carotid occlusion:a proposed management strategy显示文摘 | Rahme R Abruzzo TA Ringer AJ | | 0，，： | 1 |
| 18 | Reproductive and oncologic outcomes after progestin therapy for endomettial complex atypical hyperplasia or carcinoma显示文摘 | Kudesia R Singer T Caputo TA | 2014 | Am J Obstet Gyuecol2014，210，3： | 1 |
| 19 | New England Medical Center Posterior Circulation registry显示文摘 | Caplan LR Wityk R J Glass TA | 2004 | Ann Neurol2004，56，3： | 1 |
| 20 | Bcl-2 inhibition of neural death:decreased generation of reactive oxygen species显示文摘 | Kane DJ Sarafian TA Anton R | 1993 | Science1993，262，： | 1 |
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