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| 1 | Depletion of CD25^+CD4^+T cells (Tregs) enhances the HBV-specific CD8^+ T cell response primed by DNA immunization显示文摘AIM: Persistent hepatitis B virus (HBV) infection is characterized by a weak CD8+ T cell response to HBV. Immunotherapeutic strategies that overcome tolerance and boost these suboptimal responses may facilitate viral clearance in chronically infected individuals. Therefore, we examined whether CD25+CD4+ regulatory T (Treg) cells might be involved in a inhibition of CD8+T cell priming or in the modulation of the magnitude of the'peak' antiviral CD8+ T cell response primed by DNA immunization. METHODS: B10.D2 mice were immunized once with plasmid pCMV-S. Mice received 500 μg of anti-CD25 mAb injected intraperitoneally 3 d before DNA immunization to deplete CD25+ cells. Induction of HBV-specific CD8+ T ceils in peripheral blood mononuclear cells (PBMCs) was measured by S28-39 peptide loaded DimerX staining and their function was analyzed by intracellular IFN-γ staining.RESULTS: DNA immunization induced HBV-specific CD8+ T cells. At the peak T cell response (d 10), 7.1±2.0% of CD8+ T cells were HBV-specific after DNA immunization, whereas 12.7±3.2% of CD8+ T cells were HBV-specific in Treg-depleted mice, suggesting that DNA immunization induced more antigen-specific CD8+ T cells in the absence of CD25+ Treg cells (n = 6, P<0.05). Similarly, fewer HBVspecific memory T cells were detected in the presence of these cells (1.3±0.4%) in comparison to Treg-depleted mice (2.6±0.9%) on d 30 after DNA immunization (n = 6, P<0.01). Both IFN-γ production and the avidity of the HBV-specific CD8+ T cell response to antigen were higher in HBV-specific CD8+ T cells induced in the absence of Treg cells.CONCLUSION: CD25+ Treg cells suppress priming and/or expansion of antigen-specific CD8+ T cells during DNA immunization and the peak CD8+ T cell response is enhanced by depleting this cell population. Furthermore, Treg cells appear to be involved in the contraction phase of the CD8+ T ceil response and may affect the quality of memory T cell pools. The elimination of Treg cells or their inhibition may be important in immunotherapeutic strategies to control HBV infection by inducing virus-specific cytotoxic T lymphocyte responses in chronically infected subjects. | Yoshihiro Furuichi Hirotake Tokuyama Satoshi Ueha Makoto Kurachi Fuminori Moriyasu Kazuhiro Kakimi | 2005 | World Journal of Gastroenterology2005，11，24： | 30 |
| 2 | Utility of tricalcium phosphate and osteogenic matrix cell sheet constructs for bone defect reconstruction显示文摘AIM: To determine the effects of transplanting osteogenic matrix cell sheets and beta-tricalcium phosphate(TCP) constructs on bone formation in bone defects.METHODS: Osteogenic matrix cell sheets were prepared from bone marrow stromal cells(BMSCs), and a porous TCP ceramic was used as a scaffold. Three experimental groups were prepared, comprised of TCP scaffolds(1) seeded with BMSCs;(2) wrapped with osteogenic matrix cell sheets; or(3) both. Constructs were implanted into a femoral defect model in rats and bone growth was evaluated by radiography, histology, biochemistry, and mechanical testing after 8 wk. RESULTS: In bone defects, constructs implanted with cell sheets showed callus formation with segmentalor continuous bone formation at 8 wk, in contrast to TCP seeded with BMSCs, which resulted in bone nonunion. Wrapping TCP constructs with osteogenic matrix cell sheets increased their osteogenic potential and resulting bone formation, compared with conventional bone tissue engineering TCP scaffolds seeded with BMSCs. The compressive stiffness(mean ± SD) values were 225.0 ± 95.7, 30.0 ± 11.5, and 26.3 ± 10.6 MPa for BMSC/TCP/Sheet constructs with continuous bone formation, BMSC/TCP/Sheet constructs with segmental bone formation, and BMSC/TCP constructs, respectively. The compressive stiffness of BMSC/TCP/Sheet constructs with continuous bone formation was significantly higher than those with segmental bone formation and BMSC/TCP constructs.CONCLUSION: This technique is an improvement over current methods, such as TCP substitution, and is useful for hard tissue reconstruction and inducing earlier bone union in defects. | Tomoyuki Ueha Manabu Akahane Takamasa Shimizu Yoshinobu Uchihara Yusuke Morita Naoya Nitta Akira Kido Yusuke Inagaki Kenji Kawate Yasuhito Tanaka | 2015 | World Journal of Stem Cells2015，7，5： | 7 |
| 3 | Design of a Traveling Wave Tyjpe wave Type Ultrasonic Motor显示文摘 | Hiroshi Hirata Sadayuki Ueha | 1995 | IEEE TRANSACTIONS ON ULTRSONICS FERROELECTRICS AND FREQUENCY CONTROL1995，42，2： | 2 |
| 4 | Ultrasonic motors theory and applications显示文摘 | Ueha S | 1993 | Clarendon Press Oxford1993，，： | 1 |
| 5 | Modal vibration control of large ultrasonic tools with the use of wave-trapped horns显示文摘 | Adachi K Ueha S | 1990 | Journal of the Acoustical Society of America1990，87，1： | 1 |
| 6 | A finite-element analysis of transient vibration of an ultrasonic welding tool显示文摘 | Koike Y Ueha S | 1993 | Japanese Journal of Applied Physics1993，32，5： | 1 |
| 7 | Noncantact suspending and transporting planar objects by using acoustic levitation显示文摘 | HASHIMOTO Yoshiki KOIKE Yoshikazu UEHA Sadayuki | 1997 | Trans IEE of Japan1997，117，11： | 1 |
| 8 | Ligand-independent activation of vascular endothelial growth factor receptor 2 by fluid shear stress regulates activation of endothelial nitric oxide synthase显示文摘 | Jin ZG Ueha H Tanimoto T | 2003 | Circ Res2003，93，： | 1 |
| 9 | An analysis of a noncontact ultrasonic motor with an ultrasonically levitated rotor显示文摘 | HU J H NAKAMRA K UEHA S | 1997 | Ultrasonics1997，35，6： | 1 |
| 10 | Visualization of naturally occurring Foxp3 + regulatory T cells in normal and tumor-bearing mice显示文摘 | Hontsu S Yoneyama H Ueha S | 2004 | Int Immunopharmacol2004，4，14： | 1 |
| 11 | Visualization of naturally occurring Foxp3^+ regulatory T cells in normal and tumor-beating mice 显示文摘 | Hontsu S Yoneyama H Ueha S | 2004 | Int Immunopharmacol2004，4，14： | 1 |
| 12 | Chemokine-mediated rapid turnover of myeloid-defived suppressor cells in tumor-bearing mice 显示文摘 | Sawanobori Y Ueha S Kurachi M | 2008 | Blood2008，111，12： | 1 |
| 13 | Non-contact transportation using near-field acoustic levitation 显示文摘 | UEHA S HASHIMOTO Y KOIKE Y | 2000 | Ultrasonics2000，38，： | 1 |
| 14 | Transporting objects without contact using flexural traveling waves显示文摘 | HASHIMOTO Y KOIKE Y UEHA S | 1998 | J Acout Soc Am1998，103，6： | 1 |
| 15 | Analysis of the transformation of mechanical impact energyto electrical energy using a piezoelectric vibrator显示文摘 | UMEDA M NAKAMURA K UEHA S | 1996 | Jpn J Appl Phys1996，35，5： | 1 |
| 16 | A New ultrasonic motor using electro-rheoligical fluid and torsional vibration显示文摘 | NAKAMURA K MARUYAMA M UEHA S | 1996 | Ulrasonics1996，34，： | 1 |
| 17 | A piezoelectric micromotor using in-plane shearing of PZT elements显示文摘 | Friend J Nakamura K Ueha S | 2004 | IEEE/ASME Transactions on Mechatranoics2004，9，3： | 1 |
| 18 | Ginkgo biloba extract protects brain neurons against oxidative stress induced by hydrogen peroxide显示文摘 | Oyama Y Chikahisa L Ueha T Kanemaru K Noda K | 1996 | Brain Res1996，712，2： | 1 |
| 19 | Design of a traveling wave type ultrasonic motor显示文摘 | Hiram H Ueha S | 1995 | IEEE Transaction on Ultrasonic and Frequency Control1995，42，2： | 1 |
| 20 | Reflectivity and illuminating power compensation for optical fiber vibrometer 显示文摘 | Li X Nakamura K Ueha S | 2004 | Meas Sci Technol2004，15，： | 1 |
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