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| 1 | Cancer and age related colonic crypt deficiencies in cytochrome c oxidase Ⅰ显示文摘AIM: To investigate whether defi ciency of expressionof cytochrome c oxidase I (CcOI) in colonic crypts is associated with colon cancer.METHODS: The pattern and level of expression of CcOI in non-neoplastic colonic crypts,and in dysplastic tissues,was assessed using standard immunohis-tochemical methods.Biopsies were obtained from individuals undergoing colonoscopies for screening purposes or for a medically indicated reason.Tissue samples were also obtained from surgical colonic resections.Samples from resections were taken from colonic mucosa 1 and 10 cm from tumors and from the tumors themselves.Samples were evaluated for frequency of crypts with reduced or absent expression of CcOI.In most crypts the loss was apparent throughout the entire crypt,while in a small minority the loss was segmental.The strong immunoreactivity using this monoclonal antibody makes the scoring unambiguous.The percent of crypts with reduced or absent expression of CcOI or (infrequent) segmented loss of expression was then calculated.Data analyses were performed using SPSS statistical package 17.0.RESULTS: The average frequency of CcOI deficient crypts (CcOI-DC) is low in individuals between 20 and 39 years of age,with 0.48% ± 0.40% CcOI-DC for women and 1.80% ± 0.35% for men.CcOI-DC increases after age 40 years,so that between the ages of 40 and 44 years the average frequency of CcOI- DC goes up to 5.89% ± 0.84% in women and 2.15% ± 1.27% in men.By 80-84 years of age,the average frequency of CcOI-DC goes up in women to 15.77% ± 0.97% and in men to 22.6% ± 0.65%.The increases in CcOI-DC from ages 40-44 years compared to 80-84 years in women and men are significantly different with P < 0.01.For women over age 60 years,deficiency of CcOI expression is greater in those women who have had a cancer in their colon.The frequency of CcOI-DC,measured in men,increased in tissues adjacent to colon cancer,being 4.03% ± 0.27% in individuals free of neoplasia in the age range 55-64 yearsand 14.13% ± 0.35% in resected histologically normal tissue of men with cancer in the same age range,P < 0.001.Similar signifi cant differences were noted in older age ranges.The frequency of CcOI-DC crypts in the cecum and sigmoid colon of an individual are signifi cantly correlated,with an R2 = 0.414 for women and R2 = 0.528 for men,P < 0.001.This suggests that the factors determining the level of CcOI deficiency act throughout the colon.Most defective crypts are in clusters of two or more,a likely consequence of crypt fission.In the non-neoplastic margins of cancers,crypts are frequently defi cient for CcOI,and such crypts may appear in large clusters,some containing more than 100 defi cient crypts.CcOI defi ciency is also apparent in colon cancers and sometimes involves a large section of the tumor.Overall,CcOI deficient cells can be visualized in segments of crypts,in whole crypts that increase in frequency with age,in crypts undergoing f ission,in clusters of crypts where the clusters increase in size with age,in increased frequency near tumors,in large clusters in the intimate margins of tumors,and in the tumors themselves.There is no clear dividing line between early stages that can be considered aspects of aging and later stages that can be considered aspects of the progression to cancer.This ambiguity may re ect a rather general situation leading to adult cancer where the early stages of cellular change appear to be relatively innocuous features of the aging process but over decades may evolve into malignancy.CONCLUSION: CcOI defi cient crypts increase in frequency with age,and clusters of defi cient crypts are associated with,and may give rise to,colon cancer. | Carol Bernstein Alexander Facista Huy Nguyen Beryl Zaitlin Nadia Hassounah Cristy Loustaunau Claire Margaret Payne Bhaskar Banerjee Steve Goldschmid V Liana Tsikitis Robert Krouse Harris Bernstein | 2010 | World Journal of Gastrointestinal Oncology2010，2，12： | 5 |
| 2 | A function blocking abti-mouse integrin aipha5betal antibody inhibits angio- genesis and impedes tumor growth in vivo显示文摘 | Bhaskar V Zhang D Fox M | 2007 | J Transl Med2007，5，： | 1 |
| 3 | Monoclonal antibodies targe- ting IL-I beta reduce biomarkers of atherosclerosis in vitro and in- hibit atherescleretic plaque formation in Apolipoprotein E-deficient mice 显示文摘 | Bhaskar V Yin J Mirza AM | 2011 | Atherosclerosis2011，216，2： | 1 |
| 4 | Bacterial extracellular polymeric substance (EPS):A carrier of heavy metals in the marine food-chain显示文摘 | Bhaskar P V Bhosle N B | 2006 | Environment International2006，32，2： | 1 |
| 5 | Structure and reactivity of vanadium oxide catalysts supported on anatase rio2显示文摘 | Chary K V R Kishan G Bhaskar T | 1998 | Journal of Physical Chemistry B1998，102，35： | 1 |
| 6 | Abundance of dissolved polysaccharides in the oxygen minimum layer of the Northern Indian Ocean 显示文摘 | Bhosle N B Bhaskar P V Ramachandran S | 1998 | Marine Chemistry1998，63，： | 1 |
| 7 | A functional interaction between dorsal and components of the Smt3 conjugation machinery显示文摘 | Bhaskar V Valentine SA Courey AJ | 2000 | J Biol Chem2000，275，6： | 1 |
| 8 | Hypoglycemic effect of Mucuna pruiens seed extract on normal and streptotocin-diabetic rats显示文摘 | Bhaskar A Vidhya V G Ramya M | 2008 | Fitoterapia2008，79，： | 1 |
| 9 | Convenient methods for the reduction of amide,nitriles,carboxylic esters,acids and hydroboration of alkenes using NaBH4/I2 system显示文摘 | Bahanu Prasad A S Bhaskar Kanth J V Periasamy M | 1992 | Tetrahedron1992，48，22： | 1 |
| 10 | Biological decolorization of simulated azo dye in aqueous phase by algae spirogyra species显示文摘 | VENKATA M S BHASKAR Y V KARTHIKEYAN J | 2004 | International Journal of Environment and Pollution2004，21，3： | 1 |
| 11 | Mariappan Periasamy, Convenient methods for the reduction of amides, nitriles,carboxylic esters, acids and hydrobiration of alkenes using NaBH4/I2 system显示文摘 | Bhanu Prasad A S Bhaskar Kanth J V | 1992 | Tetrahedron1992，48，22： | 1 |
| 12 | Free Radical Scavenging Activity and Qualitative Analysis of Polyphenols by RP-HPLC in the Flowers of Couroupita guianensis Abul显示文摘 | Nagavani V Madhavi Y Rao Bhaskar D Rao Koteswara P Rao Raghava T | 2010 | Electronic Journal of Environmental Agricultural and Food Chemistry2010，9，9： | 1 |
| 13 | Optimization of spray drying of ginger extraet 显示文摘 | Jangam Sachin V Thorat Bhaskar N | 2010 | Drying Technology2010，28，12： | 1 |
| 14 | Formulation and invivo evaluation of membrane - moderated transdermal therapeutic systems of nicardipine hydrochloride using earvone as a penetration enhancer显示文摘 | Krishnaiah YSR Satyanarayana V Bhaskar P | 2003 | Drug Delivery2003，10，2： | 1 |
| 15 | The First Asymmetric Total Synthesis of (R)-Tu- berolactone, (S)-Jasmine Lactone and (R)-&decalac- tone显示文摘 | GOWRAVARAM S BHASKAR V YADAV J S | 2006 | Tetrahedron Lett2006，47，46： | 1 |
| 16 | Roothairs as specialized respiratory cells:a new hypothesis显示文摘 | Bhaskar V Berlyn G P Connolly J H | 1993 | Journal of Sustainable Forestry1993，1，： | 1 |
| 17 | Hexokinase-mitochondria interaction mediated by Akt is required to inhibit apoptosis in the presence or absence of Bax and Bak显示文摘 | Majewski N Nogueira V Bhaskar P | 2004 | Mol Cell2004，16，5： | 1 |
| 18 | Postoperative Mycobacterium chelonae Endophthalmitis after extracapsular cataract extraction and posterior chamber intraocular lens implantation显示文摘 | Ramaswamy AA Biswas J Bhaskar V | 2000 | Ophthalmology2000，107，7： | 1 |
| 19 | Characterization and Reactivity of Molybdenum Oxide Catalysts Supported on Anatase and Rutile Polymorphs of Titania显示文摘 | Chary K V R Bhaskar T Kalyana K | 2001 | Appl Catal A:Gen2001，208，12： | 1 |
| 20 | Ceramic nanoparticle dispersions:colloidal stability and coating properties显示文摘 | Jiakuan Sun Bhaskar V Velamakanni William W Gerberich | 2004 | Journal of Colloid and Interface Science2004，，280： | 1 |
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