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| 1 | Differentiation of Sheeppox and Goatpox Viruses by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism显示文摘In the present study,the partial gene sequences of P32 protein,an immunogenic envelope protein of Capripoxviruses (CaPV),were analyzed to assess the genetic relationship among sheeppox and goatpox virus isolates,and restriction enzyme specific PCR-RFLP was developed to differentiate CaPV strains.A total of six goatpox virus (GTPV) and nine sheeppox virus (SPPV) isolates of Indian origin were included in the sequence analysis of the attachment gene.The sequence analysis revealed a high degree of sequence identity among all the Indian SPPV and GTPV isolates at both nucleotide and amino acid levels.Phylogenetic analysis showed three distinct clusters of SPPV,GTPV and Lumpy skin disease virus (LSDV) isolates.Further,multiple sequence alignment revealed a unique change at G120A in all GTPV isolates resulting in the formation of Dra I restriction site in lieu of EcoR I,which is present in SPPV isolates studied.This change was unique and exploited to develop restriction enzyme specific PCR-RFLP for detection and differentiation of SPPV and GTPV strains.The optimized PCR-RFLP was validated using a total of fourteen (n=14) cell culture isolates and twenty two (n=22) known clinical samples of CaPV.The Restriction Enzyme specific PCR-RFLP to differentiate both species will allow a rapid differential diagnosis during CaPV outbreaks particularly in mixed flocks of sheep and goats and could be an adjunct/supportive tool for complete gene or virus genome sequencing methods. | Gnanavel Venkatesan Vinayagamurthy Balamurugan Revaniah Yogisharadhya Amit Kumar Veerakyathappa Bhanuprakash | 2012 | Virologica Sinica2012，27，6： | 10 |
| 2 | A Rapid and Sensitive One Step-SYBR Green Based Semi Quantitative Real Time RT-PCR for the Detection of peste des petits ruminants Virus in the Clinical Samples显示文摘敏感、快速的单个步实时(rt ) RT-PCR 用一步舞被标准化出众的格林·西布尔工具包吗?为 peste des petitis ruminants 病毒(PPRV ) 的察觉和 semi-quantitation 使用病毒 RNA 和矩阵(M) 蛋白质基因特定的教材并且与确定的常规 RT-PCR 和 TaqMan RT-PCR 相比。试金与 78.28 ~ 78.50 的 Tm 放大 PPRV M 基因的 124 bp 碎片。试金在到有 0.5 fg 的察觉限制(敏感) 的 0.5 fg 总数病毒 RNA 的 50 ng 的一个范围以内是线性的。基于现场稀释的 PPR 疫苗的病毒的连续冲淡,察觉限制是 0.0001 房间文化传染剂量 50% 单位(TCID50 ) 。另外,与疫苗的病毒的已知的 titre 刺的拖把材料同等地好在试金检测了。标准化 rt RT-PCR 容易在这块地和试验性的临床的样品里直接为 PPRV nucleic 酸的察觉被采用。试金从试验性的样品在拖把材料象 3 天柱子感染(dpi ) 和多达 20 dpi 一样早检测了 PPRV nucleic 酸。试金比在从 PPR 的 PPRV nucleic 酸的察觉的 TaqMan 和常规 RT-PCR 怀疑临床的样品绵羊和山羊的快速、更敏感。因此,确定的、简化 SYBR 绿 rt RT-PCR 是一种选择测试各种各样的诊断试金到已经存在并且能为有在减少污染的风险的优点的快速的临床的诊断有用。 | Vinayagamurthy Balamurugan Arnab Sen Gnanavel Venkatesan Vinita Yadav Vandna Bhanot Veerakyathappa Bhanuprakash Raj Kumar Singh | 2012 | Virologica Sinica2012，27，1： | 10 |
| 3 | Genetically modified mouse models for the study of nonalcoholic fatty liver disease显示文摘Nonalcoholic fatty liver disease(NAFLD) is associated with obesity,insulin resistance,and type 2 diabetes.NAFLD represents a large spectrum of diseases ranging from(1) fatty liver(hepatic steatosis);(2) steatosis with inflammation and necrosis;to(3) cirrhosis.The animal models to study NAFLD/nonalcoholic steatohepatitis(NASH) are extremely useful,as there are still many events to be elucidated in the pathology of NASH.The study of the established animal models has provided many clues in the pathogenesis of steatosis and steatohepatitis,but these remain incompletely understood.The different mouse models can be classified in two large groups.The first one includes genetically modified(transgenic or knockout) mice that spontaneously develop liver disease,and the second one includes mice that acquire the disease after dietary or pharmacological manipulation.Although the molecular mechanism leading to the development of hepatic steatosis in the pathogenesis of NAFLD is complex,genetically modified animal models may be a key for the treatment of NAFLD.Ideal animal models for NASH should closely resemble the pathological characteristics observed in humans.To date,no single animal model has encompassed the full spectrum of human disease progression,but they can imitate particular characteristics of human disease.Therefore,it is important that the researchers choose the appropriate animal model.This review discusses various genetically modified animal models developed and used in research on NAFLD. | Perumal Nagarajan M Jerald Mahesh Kuma Ramasamy Venkatesan Subeer S Majundar Ramesh C Juyal M Jerald Mahesh Kuma | 2012 | World Journal of Gastroenterology2012，18，11： | 7 |
| 4 | A review on assessment and treatment of the trunk in stroke A need or luxury显示文摘Trunk function has been identified as an important early predictor of functional outcome after stroke and the same deteriorates on both contralateral and ipsilateral sides of the body following stroke.The primary contribution of the trunk muscles is to allow the body to remain upright,adjust weight shifts,and control movements against constant pull of gravity and is considered central key point of the body.Proximal stability of the trunk is a pre-requisite for distal limb mobility,balance,gait and functional activities and its positive correlation in hemiplegia has been demonstrated in a cross-sectional study.Both isokinetic and handheld dynamometer muscle strength testing demonstrated the weakness of bilateral trunk flexors,extensors and rotator muscles in both acute and chronic hemiplegic patients.This was confirmed by electromyography analysis which identified poor bilateral trunk muscles activity in patients with stroke.Trunk impairment scale is sensitive to evaluate the selective muscle control of upper and lower trunk,and it has been reported that lateral flexion of the trunk is easier than rotation of the trunk and the clinical observation concurs to the difficulty in lower trunk rotation of stroke patients.However,trunk exercises given early after stroke could produce enhanced balance performance post-stroke.This review attempts to report the evidence supporting the involvement of the trunk and its influence on balance and functional performance in post-stroke hemiplegia. | Suruliraj Karthikbabu Mahabala Chakrapani Sailakshmi Ganeshan Kedambadi C Rakshith Syed Nafeez Venkatesan Prem | 2012 | Neural Regeneration Research2012，7，25： | 5 |
| 5 | Subcycle control of terahertz waveform polarization using all-optically induced transient metamaterials显示文摘Coherent radiation with frequencies ranging from 0.3 to 30 THz has recently become accessible using femtosecond laser technology.These terahertz(THz)waves have already been applied in spectroscopy and imaging and can be manipulated using static optical elements such as lenses,polarizers and filters.However,ultrafast modulation of THz radiation is required as well,for instance,in short-range wireless communication or for preparing shaped THz transients for the coherent control of numerous material excitations.Here,we demonstrate an all-optically created transient metamaterial that allows us to manipulate the polarization of THz waveforms with subcycle switch-on times.The polarization-modulated pulses are potentially interesting for controlling elementary motions such as the vibration of crystal lattices,the rotation of molecules and the precession of spins. | N Kamaraju Andrea Rubano Linke Jian Surajit Saha T Venkatesan Jan Notzold R Kramer Campen Martin Wolf Tobias Kampfrath | 2014 | Light(Science & Applications)2014，3，1： | 5 |
| 6 | Study on Passive Immunity:time of Vaccination in Kids Born to Goats Vaccinated Against Peste des petits ruminants显示文摘In this study,the decay of maternal peste des petits ruminants virus(PPRV) antibodies in kids born to goats vaccinated with Asian lineage IV PPR vaccine and the efficacy of passive immunity against PPRV was assessed to determine the appropriate period for vaccination in kids.Serum samples collected from kids born to vaccinated,unvaccinated and infected goats at different time intervals were tested by PPR competitive ELISA and serum neutralization test(SNT).Maternal antibodies in kids were detectable up to 6 months with a decline trend from the third month onwards and receded below the protective level by the fourth month.The kid with an SN titre of 1:8 at the time of immunization showed significant PPRV specific antibody response(percentage inhibition of 76;SN titers >1:16),when tested on 21 day post-vaccination and was completely protected from infection upon virulent PPRV challenge.Similarly,the kid with 1:8 SN titers was completely protected from PPR infection on active challenge.Therefore,PPR vaccination is recommended in kids,aged 4 months and born to immunized or exposed goats.This could be a suitable period to avoid window of susceptibility in kids to PPRV and the effort to eliminate PPR infection from susceptible populations. | Vinayagamurthy Balamurugan Arnab Sen Gnanavel Venkatesan Kaushal Kishor Rajak Veerakyathappa Bhanuprakash Raj Kumar Singh | 2012 | Virologica Sinica2012，27，4： | 5 |
| 7 | Novel degradation products of argatroban: Isolation, synthesis and extensive characterization using NMR and LC-PDA-MS/Q-TOF显示文摘Forced degradation study of argatroban under conditions of hydrolysis(neutral, acidic and alkaline), oxidation,photolysis and thermal stress, as suggested in the ICH Q1 A(R2), was accomplished. The drug showed significant degradation under hydrolysis(acidic, alkaline) and oxidation(peroxide stress) conditions. The drug remained stable under thermal and photolytic stress conditions. In total, seven novel degradation products(DP-1 to DP-7) were found under diverse conditions, which were not reported earlier. The chemical structures of these degradation products were characterized by ~1H NMR,^(13)C NMR, 2 D NMR, Q-TOF-MSnand IR spectral analysis and the proposed degradation products structures were further confirmed by the individual synthesis. | Vinodh Guvvala Venkatesan Chidambaram Subramanian Jaya Shree Anireddy Mahesh Konda | 2018 | Journal of Pharmaceutical Analysis2018，8，2： | 4 |
| 8 | Mapping of dwarfing gene Rht14 in durum wheat and its effect on seedling vigor, internode length and plant height显示文摘Short coleoptiles associated with GA-insensitive Rht-1 alleles in wheat reduces yield due to poor seedling establishment under dry, or stubble-retained conditions. Hence there is a need for alternative dwarfing genes for wheat improvement programs. GA-sensitive dwarfing gene Rht14 confers semidwarf stature in wheat while retaining longer coleoptiles and early seedling vigor. Two RIL populations were used to identify the map position of Rht14 and to estimate its effect on plant height, coleoptile length, seedling shoot length,spike length and internode length. Rht14 on chromosome 6 A was mapped in the genomic region 383–422 Mbp flanked by GA2oxA9 and wmc753 in a Bijaga Yellow/Castelporziano RIL population. Recombination events between Rht14 and GA2oxA9 in the RIL population indicated that Rht14 might not be allelic to GA2oxA9. The conserved DNA sequence of GA2oxA9 and its flanking region in Castelporziano also suggested that the point of mutation responsible for the Rht14 allele must be a few Mbp away from GA2oxA9. The dwarfing effects of Rht14 on plant height, internode length and seedling vigor were compared with those of Rht-B1 b in an HI 8498/Castelporziano RIL population. Both genes significantly reduced plant height and internode length. Rht-B1 b conferred a significant reduction in coleoptile length and seedling shoot length, whereas Rht14 reduced plant height, but not coleoptile and seedling shoot length. Therefore, Rht14 can be a used as an alternative to Rht-B1 b for development of cultivars suitable for deeper sowing in dry environments and in conditions of conservation agriculture where crop residues are retained. | Parimal Vikhe Suhasini Venkatesan Ajit Chavan Shubhada Tamhankar Ravindra Patil | 2019 | The Crop Journal2019，7，2： | 4 |
| 9 | Detection of Bluetongue Virus Group-specific Antigen Using Monoclonal Antibody Based Sandwich ELISA显示文摘A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA.The MAb,designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study.The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein.The sELISA is based on capturing of BTV antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits.The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India,1,2,15,17,18 and 23.The assay could detect BTV antigen as early as day 8 in blood.It was also successfully applied for the detection of BTV group specific antigen in clinical samples of blood,washed RBCs,buffy coat and plasma.A total of 102 field samples from animals,suspected of being infected with BTV,were tested and 29.42% were positive.The blood samples were also amplified in cell culture which improved the sensitivity of the assay.Results confirmed that the sELISA is rapid and specific. | Pradeep Narayan Gandhale Veerakyathappa Bhanuprakash Vinayagamurthy Balamurugan Madhusudhan Hosamani Gnanavel Venkatesan Raj Kumar Singh | 2010 | Virologica Sinica2010，25，6： | 4 |
| 10 | Influence of suspension stability on wet grinding for production of mineral nanoparticles显示文摘Grinding behavior of nanoparticles in an attritor mill and the minimum achievable particle size are strongly influenced by the suspension stability.In the present work,suspension stability(i.e.ζ-potential) of nanoparticles was studied by measuring pH as a function of grinding time in the wet milling process.It was found that after a certain time in an attritor mill,there is no further size reduction and the average product particle size increases monotonically.One of the reasons is that the production of submicron particles leads to more particle-particle interactions and consequently pH of the suspension decreases with grinding time.Usually pH value is related to suspension stability and it can be enhanced by addition of NaOH solution.The maximum negative ζ-potential of -51.2 mV was obtained at pH of 12 for silica.The higher the ζ-potential with the same polarity,higher will be the electrostatic repulsion between the particles.Hence,the maximum electrostatic repulsion force was maintained by the adjustment of pH value in wet milling.The experiments were conducted at different pH conditions which were maintained constant throughout the experiments and nanosized particles were obtained consequently. | S.Sakthivel Venkatesan V.Krishnan B.Pitchumani | 2008 | Particuology2008，6，2： | 4 |
| 11 | Electrochemistry during efficient copper recovery from complex electronic waste using ammonia based solutions显示文摘 | Zhi Sun Hongbin Cao Prakash Venkatesan Wei Jin Yanping Xiao Jilt Sietsma Yongxiang Yang | 2017 | Frontiers of Chemical Science and Engineering2017，11，3： | 3 |
| 12 | Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet显示文摘 | A. Abdul Rahuman Geetha Gopalakrishnan P. Venkatesan Kannappan Geetha | 2008 | Parasitology Research2008，，5： | 3 |
| 13 | Design of a kJ-class HiLASE laser as a driver for inertial fusion energy显示文摘We present the results of performance modeling of a diode-pumped solid-state HiLASE laser designed for use in inertial fusion energy power plants. The main amplifier concept is based on a He-gas-cooled multi-slab architecture similar to that employed in Mercury laser system. Our modeling quantifies the reduction of thermally induced phase aberrations and average depolarization in Yb3+:YAG slabs by a combination of helium cryogenic cooling and properly designed(doping/width) cladding materials. | Antonio Lucianetti Magdalena Sawicka Ondrej Slezak Martin Divoky Jan Pilar Venkatesan Jambunathan Stefano Bonora Roman Antipenkov Tomas Mocek | 2014 | High Power Laser Science and Engineering2014，2，2： | 2 |
| 14 | Insulin resistance in polycystic ovary syndrome:progress and paradoxes 显示文摘 | Venkatesan AM Dunaif A Corbould A | 2001 | Recent Prog Horm Res2001，56，： | 2 |
| 15 | Laboratory study on larvicidal activity of indigenous plant extracts against Anopheles subpictus and Culex tritaeniorhynchus显示文摘 | G. Elango A. Abdul Rahuman A. Bagavan C. Kamaraj A. Abduz Zahir C. Venkatesan | 2009 | Parasitology Research2009，，6： | 2 |
| 16 | Establishing the role of detoxifying enzymes in field-evolved resistance to various insecticides in the brown planthopper (Nilaparvata lugens) in South India显示文摘棕色的 planthopper (BPH ) , Nilaparvata lugens (Stål ) ,是在整个亚洲的米饭的主要害虫之一。为压制 N 的杀虫药剂的广泛的使用。lugens 导致了在这域里导致经常的控制失败的杀虫药剂抵抗的开发。现在的学习的目的是在 N 的领域人口评估抵抗。从种南方印度的状态到各种各样的杀虫药剂的主要米饭的 lugens。我们也决定了除去的活动酶(esterases [EST ] ,谷胱甘肽 S-transferases [GST ] ,并且混合功能 oxidases [MFO ]) 。抵抗的中等层次在地人口被检测到 acephate , thiamethoxam 和 buprofezin (抵抗因素 1.05-20.92 褶层, 4.52-14.99 褶层,和 1.00-18.09 褶层,分别地)作为与易受影响的紧张相比当有到 imidacloprid (抵抗因素 1.23-6.70 褶层)和完全的敏感的抵抗的底层到 etofenoprox (抵抗因素 1.05-1.66 褶层)时。当为 GST 和 MFO 比率分别地从 1.29 ~ 3.41 和 1.03 ~ 1.76 变化了时,在领域人口的 EST 活动比易受影响的紧张高 1.06 ~ 3.09 倍。EST 活动被发现被相关到 acephate 抵抗(r = 0.999, P ≥ 0.001 ) 。organophosphate, neonicotinoid,和这块地里的昆虫生长管理者(IGR ) 的高选择压力是可能的在 BPH 为抵抗正在作出贡献到多重杀虫药剂,导致控制失败。获得的结果将对为对 BPH 的有效杀虫药剂的使用的 IPM 建议有益。 | Vijayakumar Maheshwari Malathi Sushil K. Jalali Dandinashivara K. Sidde Gowda Muthugounder Mohan Thiruvengadam Venkatesan | 2017 | Insect Science2017，24，1： | 2 |
| 17 | 拟南芥CKI1基因上游转录调控因子筛选及鉴定显示文摘拟南芥CKI1(cytokininindependent 1)是双组分信号系统中一个组氨酸激酶蛋白,通过作用于下游组氨酸磷酸转移蛋白激活双组分信号通路,在调控胚囊中央细胞命运分化和发育过程中具有重要作用。然而目前对于CKI1基因上游转录调控因子还知之甚少。本研究分析了不同长度的CKI1启动子在拟南芥胚囊中的活性,并利用酵母单杂交技术对CKI1上游转录调控因子进行了筛选和鉴定。结果表明,位于内含子区域中的F5/R2片段表现出与CKI1启动子全长相一致的表达活性。进一步选取3个串联重复的F5/R2片段用于构建诱饵表达载体,同时,选取拟南芥雌蕊构建cDNA文库,通过酵母单杂交筛选获得226个阳性克隆。去除低质量及冗余重复的序列后共获得66条可读序列,其中8条序列对应的基因编码具有DNA结合功能的蛋白。研究结果为进一步揭示CKI1基因的转录调控机制提供了重要参考信息。 | 刘振宁 袁黎 Venkatesan Sundaresan 余小林 | 2019 | 遗传2019，41，5： | 2 |
| 18 | The development of metallic behavior in clusters 显示文摘 | VENKATESAN T RAJESWARI M DONG Z W | 1998 | Phil Trans R Soc1998，356，： | 2 |
| 19 | Larvicidal efficacy of five cucurbitaceous plant leaf extracts against mosquito species显示文摘 | A. Abdul Rahuman P. Venkatesan | 2008 | Parasitology Research2008，，1： | 2 |
| 20 | Design, Analysis and Installation of Offshore Instrumented Moored Data Buoy System显示文摘 | Kaliyaperumal P., Venkatesan R., Senthilkumar, P Kalaivanan, C. K. Gnanadhas, T Vedachalam, N. | 2015 | Journal of Shipping and Ocean Engineering2015，5，4： | 2 |
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