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| 1 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 2 | p53-mediated transcriptional regulation and activation of the actin cytoskeleton regulatory RhoC to LIMK2 signaling pathway promotes cell survival显示文摘响应 DNA 损坏的房间命运的中央仲裁人是 p53,它调整涉及房间周期拘捕,幸存和 apoptosis 的基因的表示。尽管 DNA 损坏开始的许多回答被描绘了,肌动朊细胞骨架管理者的角色大部分是未知的。我们现在显示出那 RhoC 和秘鲁利玛机场之代号 kinase (LIMK2 ) 2 是 genotoxic 代理人导致的直接 p53 目标基因。尽管 RhoC 和 LIMK2 在肌动朊细胞骨架规定有生长得很好的角色,我们的结果显示 LIMK2 的激活也有支持幸存的功能追随者 DNA 损坏。由调停 siRNA 的击倒或选择的药理学封锁的 LIMK 抑制敏化房间到无线电 -- 或化疗,以便当与 LIMK 抑制结合了时,当单身地管理了时,是尚不致命的治疗导致了房间死亡。我们的调查结果建议把 LIMK 禁止者与 genotoxic 治疗相结合能比单个代理人的管理更有效,并且加亮在肌动朊细胞骨架管理者和 DNA 之间的一个新奇连接导致损坏的房间幸存机制。 | Daniel R Croft Diane Crighton Michael S Samuel Filipe C Lourenco June Munro Jenifer Wood Karim Bensaad Karen H Vousden Owen J Sansom Kevin M Ryan Michael F Olson | 2011 | Cell Research2011，21，4： | 3 |
| 3 | Interferon-β induced in female genital epithelium by HIV-1 glycoprotein 120 via Toll-like-receptor 2 pathway acts to protect the mucosal barrier显示文摘More than 40%of HIV infections occur via female reproductive tract(FRT)through heterosexual transmission.Epithelial cells that line the female genital mucosa are the first line of defense against HIV-1 and other sexually transmitted pathogens.These sentient cells recognize and respond to external stimuli by induction of a range of carefully balanced innate immune responses.Previously,we have shown that in response to HIV-1 gp120,the genital epithelial cells(GECs)from upper reproductive tract induce an inflammatory response that may facilitate HIV-1 translocation and infection.In this study,we report that the endometrial and endocervical GECs simultaneously induce biologically active interferon-β(IFNβ)antiviral responses following exposure to HIV-1 that act to protect the epithelial tight junction barrier.The innate antiviral response was directly induced by HIV-1 envelope glycoprotein gp120 and addition of gp120 neutralizing antibody inhibited IFNβproduction.Interferon-βwas induced by gp120 in upper GECs through Toll-like receptor 2 signaling and required presence of heparan sulfate on epithelial cell surface.The induction of IFNβwas dependent upon activation of transcription factor IRF3(interferon regulatory factor 3).The IFNβwas biologically active,had a protective effect on epithelial tight junction barrier and was able to inhibit HIV-1 infection in TZM-bl indicator cells and HIV-1 replication in T cells.This is the first report that recognition of HIV-1 by upper GECs leads to induction of innate antiviral pathways.This could explain the overall low infectivity of HIV-1 in the FRT and could be exploited for HIV-1 prophylaxis. | Aisha Nazli Sara Dizzell Muhammad Atif Zahoor Victor H Ferreira Jessica Kafka Matthew William Woods Michel Ouellet Ali A Ashkar Michel J Tremblay Dawn ME Bowdish Charu Kaushic | 2019 | Cellular & Molecular Immunology2019，16，2： | 2 |
| 4 | Predicting is difficult,especially about the future:human resources in the new millennium显示文摘 | Woods Rovert H | 1999 | International Journal of Hospitality Management1999，18，4： | 2 |
| 5 | A wind erosion equation显示文摘 | Wood ruff N P Siddoway F H | 1965 | Proc Soil Sci Soc Am1965，，29： | 1 |
| 6 | Starch biosynthesis in developing wheat grain: evidence against the direct involvemg-at of triose phosphates in the metabolic pathway显示文摘 | Keeling P L Wood J R Tyson R H | 1988 | Plant Physiology1988，87，： | 1 |
| 7 | Decision-based Con-ceptual Design:Modeling and Navigating Hetero-geneous Design Spaces显示文摘 | Wood W H Agogino A M | 2005 | Transactions of theASME Journal of Mechanical Design2005，127，1： | 1 |
| 8 | Film formation and detachment during anodizing of Al-Mg alloys显示文摘 | ZHOU X THOMPSON G E SKELDON P WOOD G C SHIMIZU K HABAZAKI H | 1999 | Corrosion Science1999，41，8： | 1 |
| 9 | Selectivity and stoichiometry of copper oxide in propylene oxidation显示文摘 | Wood B J Wise H Yolles R S | 1969 | J Catalys1969，15，： | 1 |
| 10 | The use of bivalves as bio-indicators in the assessment of marine pollution along a coastal area 显示文摘 | Yusof A M Yanta N F Wood A K H | 2004 | Journal of Radioanalytical and Nuclear Chemistry2004，259，1： | 1 |
| 11 | Structure, mechanism and regulation of peroxiredoxins 显示文摘 | Wood Z A Schrder E Robin H J | 2003 | Trends in Biochemi- cal Sciences2003，28，1： | 1 |
| 12 | Pulmonary blasto- ma:a rare childhood malignancy显示文摘 | Senac M O Jr Wood B P Isaacs H | 1991 | Radiology1991，179，3： | 1 |
| 13 | Mechanically alloying of iron-aluminium intermetallies 显示文摘 | Fair G H Wood J V | 1993 | Powder Metallurgy1993，36，2： | 1 |
| 14 | Thrombocytopenia com- plicating treatment with intravenous glycoprotein IIb/IIIa receptor in- hibitor: a pooled analysis显示文摘 | Dasgup ta H Blankenship JC Wood GC | 2000 | Am Heart J2000，140，： | 1 |
| 15 | Quantification of MUCSAC protein in human tears显示文摘 | Zhao H Jumblatt JE Wood TO | 2001 | Cornea2001，20，8： | 1 |
| 16 | Effects of butorphanol on morphine-induced itch and analgesia in primates显示文摘 | Lee H Naughton NN Woods JH | 2007 | Anesthesiology2007，107，3： | 1 |
| 17 | Mechanical alloying of Fe-Al intermetal- lies in the DO3 composition range 显示文摘 | FAIR G H WOOD J V | 1994 | Journal of Materials Sci- ence1994，29，： | 1 |
| 18 | The role of nanobacteria in urologic disease显示文摘 | WOOD H M SHOSKES D A | 2006 | World Uro12006，24，1： | 1 |
| 19 | Gas chromatographic determination and pattern recognition analysis of methanol and fuse1 oil concentrations in whiskeys 显示文摘 | WILSON L A DING J H WOODS A E | 1991 | J AOAC lnt1991，74，： | 1 |
| 20 | I-V Characteristics of Carbon Black-Loaded Crystallinepolyethylene显示文摘 | Al-Allak H M Brinkman A W Wood J | 1993 | J Mater Sci1993，28，1： | 1 |
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