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| 1 | YKL40 expression in CD14^+ liver cells in acute and chronic injury显示文摘AIM:To demonstrate that CD14 + cells are an important source of the growth factor YKL40 in acute and chronic liver damage.METHODS:Rats were inoculated with one dose of CCl4 to induce acute damage.Liver biopsies were obtained at 0,6,12,24,48 and 72 h.For chronic damage,CCl4 was administered three days per week for 6 or 8 wk.Tissue samples were collected,and cellular populations were isolated by liver digestion and purified by cell sorting.YKL40 mRNA and protein expression were evaluated by realtime polymerase chain reaction and western blot.RESULTS:Acute liver damage induced a rapid increase of YKL40 mRNA beginning at 12 h.Expression peaked at 24 h,with a 26fold increase over basal levels.By 72 h however,YKL40 expression levels had nearly returned to control levels.On the other hand,chronic damage induced a sustained increase in YKL40 expression,with 7and 9fold higher levels at 6 and 8 wk,respectively.The pattern of YKL40 expression in different subpopulations showed that CD14+cells,which include Kupffer cells,are a source of YKL40 after acute damage at 72 h[0.09 relative expression units(REU)]as well as after chronic injury at 6 wk(0.11 REU).Hepatocytes,in turn,accounted for 0.06 and 0.01 REU after 72 h(acute)or 6 wk(chronic),respectively.The rest of the CD14cells(including T lymphocytes,B lymphocytes,natural killer and natural killer T cells) yielded 0.07 and 0.15 REU at 72 h and 6 wk,respectively.YKL40 protein expression in liver was detected at 72 h as well as 6 and 8 wk,with the highest expression relative to controls(11fold;P≤0.05)seen at 6 wk.Macrophages were stimulated by lipopolysaccharide.We demonstrate that under these conditions,these cells showed maximum expression of YKL40 at 12 h,with P<0.05 compared with controls.CONCLUSION:Hepatic CD14 + cells are an YKL40 mRNA and protein source in acute and chronic liver injury,with expression patterns similar to growth factors implicated in inflammationfibrogenesis. | Oscar Pizano-Martínez Irinea Yaez-Sánchez Pilar Alatorre-Carranza Alejandra Miranda-Díaz Pablo C Ortiz-Lazareno Trinidad García-Iglesias Adrian Daneri-Navarro Mónica Vázquez-Del Mercado Mary Fafutis-Morris Vidal Delgado-Rizo | 2011 | World Journal of Gastroenterology2011，17，33： | 3 |
| 2 | Cyclic strain inhibits Notch receptor signaling in vascular smooth muscle cells in vitro显示文摘 | Morrow D Sweeney C Birney YA | 2005 | Circ Res2005，96，： | 1 |
| 3 | 显示文摘 | Shen Z T Ya D Y Sun G X Chen X C | 1983 | Physics1983，12，： | 1 |
| 4 | Initial complaints in frontotemporal lobar degeneration显示文摘 | Pijnenburg YA Gillissen F Jonker C | 2004 | Dement Geriatr Cogn Disord2004，17，： | 1 |
| 5 | The future of ZnO light emitter显示文摘 | Look D C Clsglin B Ya | 2004 | Phys Star Sol(a)2004，201，10： | 1 |
| 6 | Global Oct4 target gene a- nalysis reveals novel downstream PTEN and TNC genes re- quired for drug-resistance and metastasis in lung cancer 显示文摘 | Tang YA Chen C H Sun HS | 2015 | Nucleic Acids Res2015，43，3： | 1 |
| 7 | Utility of nu-clear magnetic resonance spec-troscopy for pancreaticcancer studies显示文摘 | Tesiram YA Lerner M Stewart C | 2012 | Pancreas2012，41，11： | 1 |
| 8 | Bactericidal and photocatalytic activities of TiO2 thin films prepared by sol-gel and reverse micelle methods显示文摘 | Tang H Y Ya J | 2002 | J Photochem Photobiol2002，153，： | 1 |
| 9 | Fibrin glue versus sutures for conjunctival autografting in pterygium surgery:a prospective comparative study 显示文摘 | Karalezli A Kucukerdonmez C Akova YA | 2008 | Br J Ophthalmol2008，92，9： | 1 |
| 10 | Analysis of the rotary ultrasonic machining mechanism 显示文摘 | YA G QIN H W YANG S C | 2002 | J of Mater Processing Technol2002，129，13： | 1 |
| 11 | Ru l e-B a s e d Cont ex tAssessment in Smart Cities显示文摘 | Beibei Hu Theodore Patkos AbdelghaniCh i b a n i Ya c i ne A m i r a t | 2012 | Web Reasoning andRule Systems Lecture Notes in Computer Science2012，，7497： | 1 |
| 12 | Effect on astigmatism of the location of clear corneal incision in phacoemulsification of cataract 显示文摘 | Altan-Yaycioglu R Akova YA Akca S Gur S Oktem C | 2007 | J Refract Surg2007，23，5： | 1 |
| 13 | Initial complaints in frontotemporal lobar degeneration显示文摘 | Pijnenburg YA Gillissen F Jonker C | | 0，，： | 1 |
| 14 | Biomechanical regulation of hedgehog signaling in vascular smooth muscle cells in vitro and in vivo显示文摘 | Morrow D Sweeney C Birney YA et a1 | 2007 | Am J Physiol Cell Physiol2007，292，： | 1 |
| 15 | A comprehensive review of ZnO materials and devices 显示文摘 | Ozgtir U Alivov Ya I Liu C | 2005 | J Appl Phys2005，98，04： | 1 |
| 16 | Biomechanical regulation of hedgehog signaling in vas- cular smooth muscle cells in vitro and in vivo显示文摘 | Morrow D Sweeney C Birney YA Guha S Collins N Cummins PM | 2007 | Am J Physiol Cell Physiol2007，292，1： | 1 |
| 17 | A comprehensive review of ZnO materials and devices 显示文摘 | ?ZGüR ü ALIVOV YA I LIU C | 2005 | J Appl Phys2005，98，04： | 1 |
| 18 | Bayesian compressive sensing显示文摘 | Ji Shihao Xue Ya LAWRENCE C | 2008 | IEEE Transactions on Signal Processing2008，56，6： | 1 |
| 19 | On the seasonal variation of the surface ozone in Athens, Greece显示文摘 | C Varotsos K Ya Kondratyev M Efstathiou | 2000 | Atmospheric Environment2000，，2： | 1 |
| 20 | Characteristics of organic precursors and their relationship with disinfection by-products显示文摘 | Chiang P C Ko Ya Wen | 2001 | Chemosphere2001，44，5： | 1 |
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