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| 1 | PTEN encoding product:a marker for tumorigenesis and progression of gastric carcinoma显示文摘AIM: To detect the expression of PTEN encoding productin normal mucosa, intestinal metaplasia (IM), dysplasia andcarcinoma of the stomach, and to investigate its clinicalimplication in tumorigenesis and progression of gastriccarcinoma.METHODS: Formalin-fixed paraffin embedded specimens from184 cases of gastric carcinoma, their adjacent normal mucosa,IM and dysplasia were evaluated for PTEN protein expressionby SABC immunohistochemistry. PTEN expression wascompared with tumor stage, lymph node metastasis, Lauren'sand WHO's histological classification of gastric carcinoma.Expression of VEGF was also detected in 60 cases of gastriccarcinoma and its correlation with PTEN was concerned.RESULTS: The positive rates of PTEN protein were 100 %(102/102), 98.5 %(65/66), 66.7 % (4/6) and 47.8 %(88/184)in normal mucosa, IM, dysplasia and carcinoma of the stomach,respectively. The positive rates in dysplasia and carcinomawere lower than in normal mucosa and IM (P<0.01).Advanced gastric cancers expressed less frequent PTEN thanearly gastric cancer (42.9 % v567.6 %, P<0.01). The positiverate of PTEN protein was lower in gastric cancer with thanwithout lymph node metastasis (40.3 % v563.3 %, P<0.01).PTEN was less expressed in diffuse-type than in intestinal-type gastric cancer (41.5 % v557.8 %,P<0.05). Signet ringcell carcinoma showed the expression of PTEN at the lowestlevel (25.0 %, 7/28); less than well and moderatelydifferentiated ones (P<0.01). Expression of PTEN was notcorrelated with expression of VEGF (P>0.05).CONCLUSION: Loss or reduced expression of PTEN proteinoccures commonly in tumorigenesis and progression of gastriccarcinoma. It is suggested that PTEN can be an objective markerfor pathologically biological behaviors of gastric carcinoma. | Lin Yang Li-Ge Kuang Hua-Chuan Zheng Jin-Yi Li Dong-Ying Wu Su-Min Zhang Yan Xin No.4 Lab,Cancer Institute,The First Affiliated Hospital,China Medical University,Shenyang 110001,China Ying Chen Shen Yang Gynecology & Obstetrics Hospital,Shenyang 110014,China | 2003 | World Journal of Gastroenterology2003，9，1： | 127 |
| 2 | An autotrophic nitrogen removal process:Short-cut nitrification combined with ANAMMOX for treating diluted effluent from an UASB reactor fed by landfill leachate显示文摘A combined process consisting of a short-cut nitrification (SN) reactor and an anaerobic ammonium oxidation upflow anaerobic sludge bed (ANAMMOX) reactor was developed to treat the diluted effluent from an upflow anaerobic sludge bed (UASB) reactor treating high ammonium municipal landfill leachate.The SN process was performed in an aerated upflow sludge bed (AUSB) reactor (working volume 3.05 L),treating about 50% of the diluted raw wastewater.The ammonium removal efficiency and the ratio of NO 2 N to NOx-N in the effluent were both higher than 80%,at a maximum nitrogen loading rate of 1.47 kg/(m 3 ·day).The ANAMMOX process was performed in an UASB reactor (working volume 8.5 L),using the mix of SN reactor effluent and diluted raw wastewater at a ratio of 1:1.The ammonium and nitrite removal efficiency reached over 93% and 95%,respectively,after 70-day continuous operation,at a maximum total nitrogen loading rate of 0.91 kg/(m 3 ·day),suggesting a successful operation of the combined process.The average nitrogen loading rate of the combined system was 0.56 kg/(m 3 ·day),with an average total inorganic nitrogen removal efficiency 87%.The nitrogen in the effluent was mostly nitrate.The results provided important evidence for the possibility of applying SN-ANAMMOX after UASB reactor to treat municipal landfill leachate. | Jie Liu Jian'e Zuo Tang Yang Shuquan Zhu Sulin Kuang Kaijun Wang | 2010 | Journal of Environmental Sciences2010，22，5： | 26 |
| 3 | Highly Efficient A. T to G. C Base Editing by Cas9n- 3uided tRNA Adenosine Deaminase in Rice显示文摘 | Fang Yan Yongjie Kuang Bin Ren Jingwen Wang Dawei Zhang Honghui Lin Bing Yang Xueping Zhou Huanbin Zhou | 2018 | Molecular Plant2018，11，4： | 26 |
| 4 | Prevalence of deep venous thrombosis in patients with acute exacerbation of chronic obstructive pulmonary disease显示文摘长期的妨碍的肺的疾病(COPD ) 的背景尖锐恶化总是与高发生和死亡被联系。因为象固定那样的一些伴随物风险因素的存在,支气管的 superinfection,为 COPD 的尖锐恶化被招收的病人通常被认为在为静脉的 thromboembolism 的发展的中等风险。在这研究,我们从 2007 年 3 月与 COPD.Methods 的尖锐恶化在病人调查了流行和深静脉的血栓(DVT ) 的临床的表明到 2009 年 3 月, 520 个连续病人在这研究被包括。在承认上,在所有情况中的更低的极限的颜色 Doppler 超声为诊断 DVT 被执行。没有 DVT,有 DVT 的病人与那些相比 DVT 在 46 种情况(9.7%) 中被发现。在有 DVT 的病人,住院的持续时间更长(P=0.01 ) ,并且机械通风要求增加了(P < 0.001 ) 。为有 DVT 的更多的可能性的病人的另外的指示物是超过 3 天的不动(P < 0.001 ) ;肺病作为共存(P=0.01 ) ;呼吸失败类型(P=0.013 ) ;吸烟的电流(P=0.001 ) 。没有 DVT,更低的极限疼痛在与那些相比的 DVT 情况中是更普通的(34.8% 对 15.2% , P=0.01 ).Conclusions 被使不能调动超过 3 天、由肺病复杂、有的 COPD 病人的尖锐恶化呼吸失败类型,有 DVT 的更高的风险。另外, DVT 察觉了解应该在有更低的极限疼痛的情况中被增加。 | DUAN Sheng-chen YANG Yuan-hua LI Xu-yan LIANG Xiao-ning GUO Rui-jun XIE Wan-mu KUANG Tu-guang DAI Hua-ping WANG Chen | 2010 | Chinese Medical Journal2010，，12： | 24 |
| 5 | Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma显示文摘Nasopharyngeal carcinoma(NPC)is an aggressive malignancy with extremely skewed ethnic and geographic distributions.Increasing evidence indicates that targeting the tumor microenvironment(TME)represents a promising therapeutic approach in NPC,highlighting an urgent need to deepen the understanding of the complex NPC TME.Here,we generated single-cell transcriptome profiles for 7581 malignant cells and 40,285 immune cells from fifteen primary NPC tumors and one normal sample.We revealed malignant signatures capturing intratumoral transcriptional heterogeneity and predicting aggressiveness of malignant cells.Diverse immune cell subtypes were identified,including novel subtypes such as CLEC9A^+dendritic cells(DCs).We further revealed transcriptional regulators underlying immune cell diversity,and cell–cell interaction analyses highlighted promising immunotherapeutic targets in NPC.Moreover,we established the immune subtype-specific signatures,and demonstrated that the signatures of macrophages,plasmacytoid dendritic cells(pDCs),CLEC9A^+DCs,natural killer(NK)cells,and plasma cells were significantly associated with improved survival outcomes in NPC.Taken together,our findings represent a unique resource providing in-depth insights into the cellular heterogeneity of NPC TME and highlight potential biomarkers for anticancer treatment and risk stratification,laying a new foundation for precision therapies in NPC. | Yu-Pei Chen Jian-Hua Yin Wen-Fei Li Han-Jie Li Dong-Ping Chen Cui-Juan Zhang Jia-Wei Lv Ya-Qin Wang Xiao-Min Li Jun-Yan Li Pan-Pan Zhang Ying-Qin Li Qing-Mei He Xiao-Jing Yang Yuan Lei Ling-Long Tang Guan-Qun Zhou Yan-Ping Mao Chen Wei Ke-Xu Xiong Hong-Bo Zhang Shi-Da Zhu Yong Hou Ying Sun Michael Dean Ido Amit Kui Wu Dong-Ming Kuang Gui-Bo Li Na Liu Jun Ma | 2020 | Cell Research2020，30，11： | 19 |
| 6 | An EcoCity model for regulating urban land cover structure and thermal environment: Taking Beijing as an example显示文摘Urban land-use/cover changes and their effects on the eco-environment have long been an active research topic in the urbanization field. However, the characteristics of urban inner spatial heterogeneity and its quantitative relationship with thermal environment are still poorly understood, resulting in ineffective application in urban ecological planning and management.Through the integration of 'spatial structure theory' in urban geography and 'surface energy balance' in urban climatology, we proposed a new concept of urban surface structure and thermal environment regulation to reveal the mechanism between urban spatial structure and surface thermal environment. We developed the EcoCity model for regulating urban land cover structure and thermal environment, and established the eco-regulation thresholds of urban surface thermal environments. Based on the comprehensive analysis of experimental observation, remotely sensed and meteorological data, we examined the spatial patterns of urban habitation, industrial, infrastructure service, and ecological spaces. We examined the impacts of internal land-cover components(e.g., urban impervious surfaces, greenness, and water) on surface radiation and heat flux. This research indicated that difference of thermal environments among urban functional areas is closely related to the proportions of the land-cover components.The highly dense impervious surface areas in commercial and residential zones significantly increased land surface temperature through increasing sensible heat flux, while greenness and water decrease land surface temperature through increasing latent heat flux. We also found that different functional zones due to various proportions of green spaces have various heat dissipation roles and ecological thresholds. Urban greening projects in highly dense impervious surfaces areas such as commercial, transportation, and residential zones are especially effective in promoting latent heat dissipation efficiency of vegetation, leading to strongly cooling effect of unit vegetation coverage. This research indicates that the EcoCity model provides the fundamentals to understand the coupled mechanism between urban land use structure and surface flux and the analysis of their spatiotemporal characteristics. This model provides a general computational model system for defining urban heat island mitigation, the greening ratio indexes, and their regulating thresholds for different functional zones. | KUANG WenHui YANG TianRong LIU AiLin ZHANG Chi LU DengSheng CHI WenFeng | 2017 | Science China Earth Sciences2017，60，6： | 16 |
| 7 | ^(131)I-labeled metuximab combined with chemoembolization for unresectable hepatocellular carcinoma显示文摘AIM:To investigate the safety and effectiveness of combined131I-metuximab and transcatheter arterial chemoembolization(TACE)for hepatocellular carcinoma(HCC).METHODS:One hundred and eighty-five patients(159men and 26 women)with advanced HCC were enrolled in this study from February 2009 to July 2011.There were 95 patients in the combined metuximab and TACE group,and 90 patients in the TACE only group.The patients were followed for 12 mo.Clinical symptoms,blood cell counts,Karnofsky Performance Score(KPS)evaluation and therapeutic effects according to the Response Evaluation Criteria in Solid Tumors were recorded and evaluated.RESULTS:The 1-mo effective rates(complete response+partial response+stable disease)of the test group and control group were 71.23%and 38.89%,respectively(P<0.001).The 6-,9-and 12-mo survival rates were 86.42%,74.07%and 60.49%for the test group and 60.0%,42.22%and 34.44%for the control group(P<0.001).The incidence of adverse events(gastrointestinal symptoms,fever and pain)and blood cell toxicity were significantly higher for the test group than for the control group(P<0.001).No severe131Imetuximab-related complications were identified.With respect to efficacy,patients in the test group had greater improvement in tumor-related pain(P=0.014)and increase in KPS(P<0.001)than those in the control group.CONCLUSION:Combination of131I-metuximab and TACE prolonged the survival time in patients with HCC compared with TACE alone.The combination treatment was safe and effective. | Qing He Wu-Sheng Lu Yang Liu Yong-Song Guan An-Ren Kuang | 2013 | World Journal of Gastroenterology2013，19，47： | 14 |
| 8 | Formations of groundwater hydrogeochemistry in a karst system during storm events as revealed by PCA显示文摘High-frequency samples have been collected at Jiangjia Spring, the outlet of Qingmuguan underground river system (QURS) in Chongqing in late April, 2008. The variations of hydrogeochemical compositions are found responding rapidly to storm events. Principal component analysis (PCA) of the 20 variables is employed to interpret the relationships with specific processes that control the groundwater hydrogeochemical formations. Through PCA, 84.961% of the total amount information is extracted to indicate the formations of groundwater hydrogeochemical features in QURS during storm events. The first component separates the soil erosion (i.e., increases in turbidity and concentrations of Al3+, TFe, TMn, Ba2+ and NO2?), and dilution effect (i.e., decreases in specific conductance and concentrations of HCO3?, Ca2+ and Sr2+), accounting for 41.495% of the variability in the data. The second component indicates residual fertilizers and duck’s waste from farmlands (i.e., increases in specific conductance and concentrations of Na+, NO3-, PO43-,K+and Cl-), contributing to 37.449%. The dissolution of dolomite and dolomitic limestone makes up 6.017%. During the first rainfall event, the groundwater quality is mainly affected by residual fertilizers and duck’s waste from farmlands, whereas in the second rainfall event, it is mainly affected by increased turbidity and ionic concentrations caused by soil erosion. | YANG PingHeng YUAN DaoXian YUAN WenHao KUANG YingLun JIA Peng HE QiuFang | 2010 | Chinese Science Bulletin2010，55，14： | 14 |
| 9 | Differentiation of intrahepatic cholangiocarcinoma from hepatocellular carcinoma in high-risk patients: a predictive model using contrast-enhanced ultrasound显示文摘AIM To develop a contrast-enhanced ultrasound(CEUS)predictive model for distinguishing intrahepatic cholangiocarcinoma(ICC) from hepatocellular carcinoma(HCC) in high-risk patients.METHODS This retrospective study consisted of 88 consecutive high-risk patients with ICC and 88 high-risk patients with HCC selected by propensity score matching between May 2004 and July 2016. Patients were assigned to two groups, namely, a training set and validation set, at a 1:1 ratio. A CEUS score for diagnosing ICC was generated based on significant CEUS features. Then, a nomogram based on the CEUS score was developed, integrating the clinical data. The performance of the nomogram was then validated and compared with that of the LR-M of the CEUS Liver Imaging Reporting and Data System(LI-RADS).RESULTS The most useful CEUS features for ICC were as follows: rim enhancement(64.5%), early washout(91.9%), intratumoral vein(58.1%), obscure boundary of intratumoral non-enhanced area(64.5%), and marked washout(61.3%, all P < 0.05). In the validation set, the area under the curve(AUC) of the CEUS score(AUC = 0.953) for differentiation between ICC and HCC was improved compared to the LI-RADS(AUC = 0.742)(P < 0.001). When clinical data were added, the CEUS score nomogram was superior to the LI-RADS nomogram(AUC: 0.973 vs 0.916, P = 0.036, Net Reclassification Improvement: 0.077, Integrated Discrimination Index: 0.152). Subgroup analysis demonstrated that the CEUS score model was notably improved compared to the LIRADS in tumors smaller than 5.0 cm(P < 0.05) but not improved in tumors smaller than 3.0 cm(P > 0.05).CONCLUSION The CEUS predictive model for differentiation between ICC and HCC in high-risk patients had improved discrimination and clinical usefulness compared to the CEUS LIRADS. | Li-Da Chen Si-Min Ruan Jin-Yu Liang Zheng Yang Shun-Li Shen Yang Huang Wei Li Zhu Wang Xiao-Yan Xie Ming-De Lu Ming Kuang Wei Wang | 2018 | World Journal of Gastroenterology2018，24，33： | 14 |
| 10 | Chitosan hydrogel incorporated with dental pulp stem cell-derived exosomes alleviates periodontitis in mice via a macrophage-dependent mechanism显示文摘Periodontitis is caused by host immune-inflammatory response to bacterial insult.A high proportion of proinflammatory macrophages to anti-inflammatory macrophages leads to the pathogenesis of periodontitis.As stem cell-derived exosomes can modulate macrophage phenotype,dental pulp stem cell-derived exosomes(DPSC-Exo)can effectively treat periodontitis.In this study,we demonstrated that DPSC-Exo-incorporated chitosan hydrogel(DPSC-Exo/CS)can accelerate the healing of alveolar bone and the periodontal epithelium in mice with periodontitis.Gene Ontology(GO)term enrichment analysis showed that treatment with DPSC-Exo/CS ameliorated periodontal lesion by suppressing periodontal inflammation and modulating the immune response.Specifically,DPSC-Exo/CS facilitated macrophages to convert from a pro-inflammatory phenotype to an anti-inflammatory phenotype in the periodontium of mice with periodontitis,the mechanism of which could be associated with miR-1246 in DPSC-Exo.These results not only shed light on the therapeutic mechanism of DPSCExo/CS but also provide the basis for developing an effective therapeutic approach for periodontitis. | Zongshan Shen Shuhong Kuang Yong Zhang Mingmei Yang Wei Qin Xuetao Shi Zhengmei Lin | 2020 | Bioactive Materials2020，5，4： | 14 |
| 11 | Cropland redistribution to marginal lands undermines environmental sustainability显示文摘Cropland redistribution to marginal land has been reported worldwide;however,the resulting impacts on environmental sustainability have not been investigated sufficiently.Here we investigated the environmental impacts of cropland redistribution in China.As a result of urbanization-induced loss of high-quality croplands in south China(~8.5 t ha^(-1)),croplands expanded to marginal lands in northeast(~4.5 t ha^(-1)) and northwest China(~2.9 t ha^(-1)) during 1990-2015 to pursue food security.However,the reclamation in these low-yield and ecologically vulnerable zones considerably undermined local environmental sustainability,for example increasing wind erosion(+3.47%),irrigation water consumption(+34.42%),fertilizer use(+20.02%) and decreasing natural habitats(-3.11%).Forecasts show that further reclamation in marginal lands per current policies would exacerbate environmental costs by 2050.The future cropland security risk will be remarkably intensified because of the conflict between food production and environmental sustainability.Our research suggests that globally emerging reclamation of marginal lands should be restricted and crop yield boost should be encouraged for both food security and environmental benefits. | Wenhui Kuang Jiyuan Liu Hanqin Tian Hao Shi Jinwei Dong Changqing Song Xiaoyong Li Guoming Du Yali Hou Dengsheng Lu Wenfeng Chi Tao Pan Shuwen Zhang Rafiq Hamdi Zherui Yin Huimin Yan Changzhen Yan Shixin Wu Rendong Li Jiuchun Yang Yinyin Dou Wenbin Wu Liqiao Liang Bao Xiang Shiqi Yang | 2022 | National Science Review2022，9，1： | 13 |
| 12 | Clinical characteristics and prognosis of community-acquired pneumonia in autoimmune disease-induced显示文摘BACKGROUND:Community-acquired pneumonia(CAP)in autoimmune diseases(AID)-induced immunocompromised host(ICH)had a high incidence and poor prognosis.However,only a few studies had determined the clinical characteristics of these patients.Our study was to explore the characteristics and predictors of mortality in CAP patients accompanied with AID-induced ICH.METHODS:From 2013 to 2018,a total of 94 CAP patients accompanied with AID-induced ICH,admitted to Emergency Department of Zhongshan Hospital,Fudan University,were enrolled in this study.Clinical data and the risk regression estimates of repeated predictors were evaluated by generalized estimating equations(GEEs)analysis.An open-cohort approach was used to classify patient's outcomes into the survival or non-survival group.RESULTS:The hospital mortality of patients with CAP occurring in AID-induced ICH was 60.64%.No significant differences were found with respect to clinical symptoms and lung images between survival and non-survival groups,while renal insufficiency and dysfunction of coagulation had higher proportions in non-survival patients(P<0.05).Both noninvasive ventilation(NIV)and invasive mechanical ventilation(IMV)were performed more frequently in non-survival group(P<0.05).By the multivariate GEEs analysis,the repeated measured longitudinal indices of neutrophilto-lymphocyte ratio(NLR)(odds ratio[OR]=1.055,95%confidence interval[95%CI]1.025–1.086),lactate dehydrogenase(LDH)(OR=1.004,95%CI 1.002–1.006)and serum creatinine(s Cr)(OR=1.018,95%CI 1.008–1.028),were associated with a higher risk of mortality.CONCLUSION:The CAP patients in AID-induced ICH had a high mortality.A significant relationship was demonstrated between the factors of NLR,LDH,s Cr and mortality risk in these patients. | Zhong-shu Kuang Yi-lin Yang Wei Wei Jian-li Wang Xiang-yu Long Ke-yong Li Chao-yang Tong Zhan Sun Zhen-ju Song | 2020 | World Journal of Emergency Medicine2020，11，3： | 12 |
| 13 | The mechanisms of traditional Chinese medicine underlying the prevention and treatment of atherosclerosis显示文摘Atherosclerosis(AS) is a chronic inflammatory disease associated with high morbidity and mortality. The incidence of AS is increasing in the last decades. So development of safe and effective therapeutics for treating AS has become prominently important. Although there are numerous chemical drugs available for treating AS, some drugs are not effective and some have serious side effects. Traditional Chinese medicine(TCM) has a long history for the prevention and treatment of AS due to its less side effects and superior efficacy. This paper describes the effectiveness and underlying mechanisms for prevention and treatment of AS by TCM or its active components. Some TCM, e.g. XuemaiNing, Tongxinluo and Salvia miltiorrhiza have been reported to have cardio-protective effect. Some active components of TCM, e.g. saikosaponin-A, kuwanon G, luteolin and β-elemene have been isolated from various TCM and demonstrated to have beneficial effects on prevention and treatment of AS. | LI Ting-Ting WANG Zhi-Bin LI Yang CAO Feng YANG Bing-You KUANG Hai-Xue | 2019 | Chinese Journal of Natural Medicines2019，17，6： | 12 |
| 14 | Remote sensing-based artificial surface cover classification in Asia and spatial pattern analysis显示文摘Artificial surfaces, characterized with intensive land-use changes and complex landscape structures, are important indicators of human impacts on terrestrial ecosystems. Without high-resolution land-cover data at continental scale, it is hard to evaluate the impacts of urbanization on regional climate, ecosystem processes and global environment. This study constructed a hierarchical classification system for artificial surfaces, promoted a remote sensing method to retrieve subpixel components of artificial surfaces from 30-m resolution satellite imageries(Globe Land30) and developed a series of data products of high-precision urban built-up areas including impervious surface and vegetation cover in Asia in 2010. Our assessment, based on multisource data and expert knowledge, showed that the overall accuracy of classification was 90.79%. The mean relative error for the impervious surface components of cities was 0.87. The local error of the extracted information was closely related to the heterogeneity of urban buildings and vegetation in different climate zones. According to our results, the urban built-up area was 18.18×104 km2, accounting for 0.59% of the total land surface areas in Asia; urban impervious surfaces were 11.65×104 km2, accounting for 64.09% of the total urban built-up area in Asia. Vegetation and bare soils accounted for 34.56% of the urban built-up areas. There were three gradients: a concentrated distribution, a scattered distribution and an indeterminate distribution from east to west in terms of spatial pattern of urban impervious surfaces. China, India and Japan ranked as the top three countries with the largest impervious surface areas, which respectively accounted for 32.77%, 16.10% and 11.93% of the urban impervious surface area of Asia. We found the proportions of impervious surface and vegetation cover within urban built-up areas were closely related to the economic development degree of the country and regional climate environment. Built-up areas in developed countries had relatively low impervious surface and high public green vegetation cover, with 50–60% urban impervious surfaces in Japan, South Korea and Singapore. In comparison, the proportion of urban impervious surfaces in developing countries is approaching or exceeding 80% in Asia. In general, the composition and spatial patterns of built-up areas reflected population aggregation and economic development level as well as their impacts on the health of the environment in the sub-watershed. | KUANG WenHui CHEN LiJun LIU JiYuan XIANG WeiNing CHI WenFeng LU DengSheng YANG TianRong PAN Tao LIU AiLin | 2016 | Science China Earth Sciences2016，59，9： | 12 |
| 15 | TNF-α and IL-1RA Polymorphisms and Silicosis Susceptibility in Chinese Workers Exposed to Silica Particles:A Case-Control Study显示文摘Objective To assess the association of TNF- α and IL-1RA SNPs with the risk of silicosis in Chinese workers exposed to silica particles. Methods Case-control study design was used to enroll 68 silicotic patients induced by silica particles and 68 healthy workers matched for length of silica particle exposure as controls. Both cases and controls were from the same company in southwest China, and each of them was requested to complete a questionnaire. Blood samples were drawn for genomic DNA extraction from each participant. The genotyping of TNF-α (-238 and -308) and IL-1RA (+2018) was performed using polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) and SYBR green-based quantitative polymerase chain reaction (qPCR), respectively. Unconditional logistic regression model was used to estimate odds ratios (ORs) and their 95% confidential intervals (CI) for SNPs. Results No significant differences were found between cases and controls in particles exposure length, body mass index (BMI), and status of smoking and alcohol consumption except for age (P=0.001) and blood type (P=0.042). The frequencies of TNF-α (-238) and IL-1RA (+2018) genotypes in cases were significantly different from those in controls, (P=0.001 and P=0.002, respectively), while a borderline significant difference was found in the frequencies of TNF-α (-308) between cases and controls (P=0.063). The variants of three SNPs increased the risk of silicosis in the Chinese workers exposed to silica particles. The adjusted ORs of TNF-α (-308), TNF-α (-238) and IL-1RA (+2018) were 2.8 (95% CI: 1.1-7.5), 20.9 (95% CI: 1.8-236.4) and 4.0 (95% CI: 1.6-10.1), respectively. Conclusion It is suggested that cytokine polymorphisms of TNF- α (-238, -308) and IL-1RA (+2018) are associated with the risk of silicosis in the Chinese workers exposed to silica particles. Further independent studies on the interaction between SNPs and exposure to silica particles with a larger sample size are therefore warranted. | WANG Yong Wei LAN Ya Jia YANG Yue Lin WANG De Jun KUANG Jie | 2012 | Biomedical and Environmental Sciences2012，25，5： | 11 |
| 16 | Microalloying Al alloys with Sc:a review显示文摘As a kind of important light alloys,the Al alloys exhibit mechanical properties that are closely related to the microstructures.Changing the main alloying elements and adjusting heat treatments are usually approaches to tune the microstructure and hence artificially control the mechanical properties.However,the windows for the two approaches have become quite narrow,after extensive studies in the last half of century.Microalloying has become the most promising strategy to further modify the microstructure and improve the mechanical properties of Al alloys,among which the element of scandium(Sc)is especially powerful.In this paper,the recent progresses in Al alloys microalloyed with Sc are briefly reviewed,focusing on the microstructural characterization,strengthening response,and underlying mechanisms.The possible key research points are also proposed for the further development of Al alloys microalloyed with Sc and other rare earth elements. | Jin-Yu Zhang Yi-Han Gao Chong Yang Peng Zhang Jie Kuang Gang Liu Jun Sun | 2020 | Rare Metals2020，39，6： | 10 |
| 17 | Development of a core set of SNP markers for the identifi cation of upland cotton cultivars in China显示文摘Considering the advantages of single nucleotide polymorphisms(SNP) in genotyping and variety identification, the first set public SNP markers at Cotton Marker Database(http://gffzz743c1d093f394620hxbupxw5ufqnq6fqo.ffgz.tsg.suse.edu.cn/) were validated and screened across standard varieties of cotton distinctness, uniformity and stability(DUS) test, aiming to obtain an appropriate set of core SNP markers suitable for upland cotton cultivars in China. A total of 399 out of 1 005 SNPs from 270 loci including 170 insertions-deletions(In Dels) were evaluated for their polymorphisms among 30 standard varieties using Sanger sequencing. As a result, 147 loci were sequenced successfully, 377 SNPs and 49 In Dels markers were obtained. Among the 377 SNP markers, 333 markers(88.3%) were polymorphic between Gossypium hirsutum and G. barbadense, while 164 markers(43.5%) were polymorphic within upland cotton. As for In Del markers, the polymorphic rate is relatively lower than that of SNP both between species and within species. The homozygous DNA locus ratio of 121 SNPs was higher than 86.2% while that of other 43 SNPs was less than 70%. Only 64 SNPs displayed completely homozygous genotypes among all of the detected upland cotton varieties with 100% homozygous DNA locus ratio. At last, a set of 23 pairs of core SNPs were achieved in view of avoidance of linkage, with polymorphism information content(PIC) values varying from 0.21 to 0.38 with an average of 0.28. Genotype characteristics and genetic diversity were analyzed based on the set of core markers, while 40 pairs of core simple-sequence repeats(SSR) primers comprised of 10 sets of four multiplex PCR combinations were also used for analysis based on fluorescence detection system. Comparison results indicated that the genetic diversity level was almost equal, while various varieties were significantly different from each other. Genetic relationship revealed by SSR markers is related to geographic source to a certain extent. Meanwhile clustering results analyzed by SNP markers are more consistent with kinship, which demonstrated that the screen strategy for core SNP marker is effective. | KUANG Meng WEI Shou-jun WANG Yan-qin ZHOU Da-yun MA Lei FANG Dan YANG Wei-hua MA Zhi-ying | 2016 | Journal of Integrative Agriculture2016，15，5： | 10 |
| 18 | Grinding force and surface quality in creep feed profile grinding of turbine blade root of nickel-based superalloy with microcrystalline alumina abrasive wheels显示文摘Creep feed profile grinding of the fir-tree blade root forms of single crystal nickel-based superalloy was conducted using microcrystalline alumina abrasive wheels in the present study. The grinding force and the surface quality in terms of surface topography, subsurface microstructure,microhardness and residual stress obtained under different grinding conditions were evaluated comparatively. Experimental results indicated that the grinding force was influenced significantly by the competing predominance between the grinding parameters and the cross-sectional root workpiece profile. In addition, the root workpiece surface, including the root peak and valley regions, was produced with the large difference in surface quality due to the nonuniform grinding loads along the root workpiece profile in normal section. Detailed results showed that the surface roughness, subsurface plastic deformation and work hardening level of the root valley region were higher by up to25%, 20% and 7% in average than those obtained in the root peak region, respectively, in the current investigation. Finally, the superior parameters were recommended in the creep feed profile grinding of the fir-tree blade root forms. This study is helpful to provide industry guidance to optimize the machining process for the high-valued parts with complicated profiles. | Qing MIAO Wenfeng DING Weijie KUANG Changyong YANG | 2021 | Chinese Journal of Aeronautics2021，34，2： | 10 |
| 19 | Synthesis, Crystal Structure and Electrochemical Properties of a One-dimensional Chain Coordination Polymer [Cu(phen)(2,4,6-TMBA)_2(H_2O)]_n显示文摘A one-dimensional chain coordination polymer [Cu(phen)(2,4,6-TMBA)2(H2O)]n has been synthesized by reacting 2,4,6-trimethyl-benzoic acid, 1,10-phenanthroline and Cu(II) per- chlorate and its structure was characterized. Crystal data for this complex: tetragonal, space group I41, a = 2.0293(3), b = 2.0293(3), c = 1.3758(2) nm, α = β = γ = 90°, V = 5.6657(13) nm3, Dc = 1.379 g/cm3, Z = 8, μ(MoKα) = 0.815 mm?1, Mr = 588.14, F(000) = 2456, S = 1.047, R = 0.0459 and wR = 0.1053. The crystal structure shows that two neighboring Cu(Ⅱ) ions are linked together by one bridging-chelating 2,4,6-trimethyl-benzoic group, forming a one-dimensional chain structure. Each Cu(Ⅱ) ion is coordinated with two nitrogen atoms from one 1,10-phenanthroline molecule, three oxygen atoms from three 2,4,6-trimethyl-benzoic acid molecules and one oxygen atom from one water molecule, giving a six-coordinate distorted octahedral coordination geometry. The cyclic voltammetry behavior of the complex was also investigated. | LI Wei LI Chang-Hong YANG Ying-Qun KUANG Yun-Fei | 2008 | Chinese Journal of Structural Chemistry2008，27，2： | 10 |
| 20 | Generation of seed lipoxygenase-free soybean using CRISPR-Cas9显示文摘Beany flavor induced by three lipoxygenases(LOXs, including LOX1, LOX2, and LOX3)restricts human consumption of soybean. It is desirable to generate lipoxygenase-free new mutant lines to improve the eating quality of soybean oil and protein products. In this study, a pooled clustered regularly interspaced short palindromic repeats(CRISPR)-CRISPRassociated protein 9(Cas9) strategy targeting three GmLox genes(GmLox1, GmLox2, and GmLox3) was applied and 60 T_0 positive transgenic plants were generated, carrying combinations of sg RNAs and mutations. Among them, GmLox-28 and GmLox-60 were gmlox1 gmlox2 gmlox3 triple mutants and GmLox-40 was a gmlox1 gmlox2 double mutant.Sequencing of T_1 mutant plants derived from GmLox-28, GmLox-60, and GmLox-40 showed that mutation in the GmLox gene was inherited by the next generation. Colorimetric assay revealed that plants carrying different combinations of mutations lost the corresponding lipoxygenase activities. Transgene-free mutants were obtained by screening the T_2 generation of lipoxygenase-free mutant lines(GmLox-28 and GmLox-60). These transgeneand lipoxygenase-free mutants could be used for soybean beany flavor reduction without restriction by regulatory frameworks governing transgenic organisms. | Jie Wang Huaqin Kuang Zhihui Zhang Yongqing Yang Long Yan Mengchen Zhang Shikui Song Yuefeng Guan | 2020 | The Crop Journal2020，8，3： | 9 |
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