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| 1 | Expression of co-stimulator 4-1BB molecule in hepatocellular carcinoma and adjacent non-tumor liver tissue,and its possible role in tumor immunity显示文摘AIM:To investigate the expression of 4-1BB molecule in hepatocellular carcinoma (HCC) and its adjacent tissues.METHODS:Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the gene expression of 4-1BB in hepatocarcinoma and its adjacent tissues,and peripheral blood mononuclear cells (PBMCs) from both HCC and health control groups.Flow cytometry was used to analyse the phenotypes of T cell subsets from the blood of HCC patients and healthy volunteers,and further to determine whether 4-1BB molecules were also expressed on the surface of CD4+ and CD8+ T cells. The localization of 4-1BB proteins on tumor infiltrating T cells was determined by direct immunofluorescence cytochemical staining and detected by confocal microscopy.RESULTS:4-1BB mRNA, which was not detectable in normal liver,was found in 19 liver tissues adjacent to tumor edge(<1.0cm).Low expression of 4-1BB mRNA was shown in 8 tumor tissues and 6 liver tissues located within 1 to 5cm away from tumor edge. In PBMCs, 4-1BB mRNA was almost not detected. Percentage of CD4^+, CD8^+ and CD3^+/CD25^+ T cells, as well as ratio of CD4 to CD8 revealed no difference between groups (P>0.05,respectively),while a significant lower percentage of CD3^+ T cell was found in HCC group as compared to healthy control group (P<0.05).However, 4-1BB molecules were almost not found on the surface of CD4+ and CD8+ T cells in HCC and healthy control group.Double-staining of 4-1BB^+/CD4^+ and 4-1BB^+/CD8^+ immunofluorescence on tumor infiltrating T cells was detected in 13 liver tissues adjacent to tumor edge (<1.0cm) by confocal microscopy.CONCLUSION: Although HCC may escape from immune attack by weak immunogenicity or downregulated expression of MHC-1 molecules on the tumor cell surface,tumor infiltrating T cells can be activated via other costimulatory signal pathways to exert a limited antitumor effect on local microenvironment. The present study also implicates that modulating 4-1BB/4-1BBL costimulatory pathway may be an effective immunotherapy strategy to augment the host response. | Wan, YL Zheng, SS Zhao, ZC Li, MW Jia, CK Zhang, H | 2004 | World Journal of Gastroenterology2004，10，2： | 6 |
| 2 | Three - and 4 - dimensional ultrasonography in the prenatal evaluation of fetal anomalies associated with trisomy 18显示文摘 | Zheng Y Zhou XD Zhu YL | 2008 | J Ultrasound Med2008，27，7： | 1 |
| 3 | Individual function difference of Runx2 isoforms in bone formation by RNA inferen显示文摘 | Li YL INi ZJ Zheng SP | 2008 | Cell Biol Int2008，32，3： | 1 |
| 4 | Brief communication : body massindex, body adiposity index, and percent body fat in asians显示文摘 | Zhao DP Li YL Zheng LB | 2013 | AmJ Physical Anthropol2013，152，2： | 1 |
| 5 | An epididymis-specific beta-defensin is important for the initiation of sperm maturation显示文摘 | Zhou CX Zhang YL Xiao L Zheng M Leung KM Chan MY Lo PS Tsang LL Wong HY Ho LS | | 0，，05： | 1 |
| 6 | Risk factors and mortality of pa- tients with nosoeomial earbapenem-resistant Aeinetobacter baumannii pneumonia 显示文摘 | Zheng YL Wan YF Zhou LY | 2013 | American journal of infection control2013，41，7： | 1 |
| 7 | Purple sweet potato color protects mouse liver against d-galactose-induced apoptosis via inhibiting caspase-3 activation and enhancing PI3K/Akt pathway显示文摘 | Zhang ZF Lu J Zheng YL | 2010 | Food Chem Toxicol2010，48，89： | 1 |
| 8 | A CDK5 inhibitory peptide reduces tau hyperphosphory-lation and apoptosis in neurons 显示文摘 | Zheng YL | 2005 | Embo J2005，24，1： | 1 |
| 9 | Population genetic data of 15 STR loci in Han population of Henan province ( central China) 显示文摘 | Zeng ZS Zheng XD Zhu YL | 2007 | Leg Med2007，9，1： | 1 |
| 10 | A novel,potential mi-croflora-activated carrier for a colon-specific drug deliv-ery system and its characteristics显示文摘 | Wang MJ Xie YL Zheng QD | 2009 | Ind Eng Chem Res2009，，： | 1 |
| 11 | Characterization of hepatic tumors:value of contrast-enhanced coded phase-inversion harmonic angio显示文摘 | Wen YL Kudo M Zheng RQ | 2004 | Am J Roentgenol2004，182，： | 1 |
| 12 | N9 microglial cells polarized byLPS and IL4 show differential responses to secondary environmentalstimuli显示文摘 | Liu HC Zheng MH Du YL | 2012 | Cell Immunol2012，278，12： | 1 |
| 13 | Effects of gut barrierdysfunction and NFaxB activation on aggravating mechanismof severe acute pancreatitis显示文摘 | Wang YL Zheng YJ Zhang ZP | 2009 | Dig Dis2009，10，1： | 1 |
| 14 | QT interval prolonga- tion associated with Intramuscular Ziprasidone in Chi- nese patients:a case report and a comprehensive litera- ture teview with Meta-Analysis 显示文摘 | Li XB Tang YL Zheng W | 2014 | Case Rep Psychia- try2014，2014，48： | 1 |
| 15 | Ursolic acid ameliorates cognition deficits and attenuates oxidative damage in the brain of senescent mice induced by D - galaetose 显示文摘 | Lu J Zheng YL Wu DM | 2007 | Biochem Pharmacol2007，74，： | 1 |
| 16 | Purple sweet potato color repairs d-galactose-induced spatial learning and memory impairment by regulating the expression of synaptic proteins显示文摘 | Wu DM Lu J Zheng YL | 2008 | Neurobiol Learn Mem2008，90，1： | 1 |
| 17 | A trtmcated peptide from p35, a Cdk5 activator, prevents Alzheimer's disease phenotypes in model mice显示文摘 | Shukla V Zheng YL Mishra SK | 2013 | FASEB J2013，27，1： | 1 |
| 18 | Characterization of hepatic tumors:value of contrast-enhanced eoded phase-inversion harmonic angio显示文摘 | Wen YL Kudo M Zheng RQ | 2004 | AJR2004，182，： | 1 |
| 19 | Troxerutin counteracts domoic acid-induced memory deficits in mice by in- hibiting CCAAT/enhancer binding protein 显示文摘 | Lu J Wu DM Zheng YL | 2013 | J Immunol2013，190，7： | 1 |
| 20 | Changes in Cesarean delivery rates over 20 years and perinatal mortality显示文摘 | Xia YL Zheng WH WS W | 2006 | China Journal of Eugenics Genetics2006，14，12： | 1 |
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