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		题名	作者	年代	出处	被引量
	1	Initial research on transferring TUA-6 gene from Arabidopsis thaliana into tobacco mediated by Agrobacterium tumefaciens显示文摘Microtubule cytoskeleton plays an important role in cell division,differentiation,cell morphology decision,introcellular material transportation and other physiological activities.Performing those function depends on tubulin,microtubule-associated proteins(MAPs),correlated kinase and phosphate(ester) enzyme.In this article,GFP-TUA-6 and TUA-6-GFP gene were transferred into tobacco cotyledon mediated by Agrobacterium tumefaciens.Transformed lines were generated by the optimized genetic transformation system.It was proved that GFP-TUA-6 and TUA-6-GFP gene had been integrated into the tobacco genome by PCR detection and Southern blot analysis.The observation of laser scanning confocal microscope showed that GFP-TUA-6 protein was mainly located in cortical microtubules,while TUA-6-GFP protein was mainly located in cytoplasm and cortical microtubules,which provide a fundament for studying the dynamics activity of microtubule in cell morphogenesis.	LIANG Feng ZHU Zhifang YANG Qing	2012	商丘师范学院学报2012，28，6：	0

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