Recombinant scorpion insectotoxin AaIT kills specifically insect cells but not human cellss

查看全文 作　　者：SHENG JIAN JI, FENG LIU, ER Qiu LI, Yu XIAN ZHUThe National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China

出　　处：《Cell Research》索引2002年第12卷第2期,共8页

基　　金：This work was supported by a grant from 863High Technology Program,Chinese Ministry of Sci-ence and Technology 摘　　要：The nucleotide sequence deduced from the amino acid sequence of the scorpion insectotoxin AaIT was chemically synthesized and was expressed in Escherichia coli. The authenticity of this in vitro expressed peptide was confirmed by N-terminal peptide sequencing. Two groups of bioassays, artificial diet incorporation assay and contact insecticidal effect assay, were carried out separately to verify the toxicity of this recombinant toxin. At the end of a 24 h experimental period, more than 60% of the testing diamondback moth (Plutella xylostella) larvae were killed in both groups with LCs0 value of 18.4 uM and 0.70 μM respectively. Cytotoxicity assay using cultured Sf9 insect cells and MCF-7 human cells demonstrated that the toxin AaIT had specific toxicity against insect cells but not human cells. Only 0.13 μM recombinant toxin was needed to kill 50% of cultured insect cells while as much as 1.3μM toxin had absolutely no effect on human cells. Insect cells produced obvious intrusions from their plasma membrane before broken up. We infer that toxin AaIT bind to a putative sodium channel in these insect cells and open the channel persistently, which would result in Na+ influx and finally cause destruction of insect cells. 关键词：SCORPION toxin, AaIT, PROKARYOTIC expression, cytotoxicity.

相关文献

参考文献（30）
引证文献（6）
耦合文献（252）

参考文献(30)

引证文献(6)

耦合文献(252)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Recombinant scorpion insectotoxin AaIT kills specifically insect cells but not human cellss

参考文献(30)

引证文献(6)

耦合文献(252)

用户提示