Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils

查看全文 作　　者：Xinyu LI[a], Huiwen ZHANG[a], Minna WU[a][b], Zhencheng SU[a];Chenggang ZHANG[a]

机构地区：[a]Microbiolog Resource;Ecology Group, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China [b]Graduate School of Chinese Academy of Sciences, Beijing 100039, China

出　　处：《Journal of Environmental Sciences》索引2008年第20卷第9期,共6页

基　　金：the Natural Science Foun-dation for Young Scientists of China (No. 40701088) 摘　　要：Acetochlor is an increasingly used herbicide on corn in North China. Currently, the effect of acetochlor on soil ammonia-oxidizing bacteria (AOB) communities is not well documented. Here, we studied the diversity and community composition of AOB in soil amended with three concentrations of acetochlor (50, 150, 250 mg/kg) and the control (0 mg acetochlor/kg soil) in a microcosm experiment by PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and the phylogenetic analysis of excised DGGE bands. DGGE profiles showed that acetochlor had a stimulating effect on AOB at the early stage after acetochlor amended, and the order of intensity and duration is medium-acetochlor amended samples (AM) > low-acetochlor amended samples (AL) > high-acetochlor amended samples (AH). At the end of 60 d microcosm, acetochlor had a negative effect on the diversity of AOB. Cluster analysis of DGGE profiles showed that acetochlor had a greater effect on the community structure of AOB on day 60 than on day 1. The phylogenetic analysis revealed that all the sequences of excised DGGE bands were closely related to members of the genus Nitrosospira and formed two separate subclusters designated as subcluster 1 and subcluster 2 affiliated respectively with clusters 3 and 4 in Nitrosospira as defined by Stephen. Some dominant AOB had a change from subcluster 2 to subcluster 1 with the incubation. The results showed that acetochlor had an effect on the AOB on a long-term basis and the chronic effect of acetochlor should be paid more attention in future. 关键词：氧化氨的细菌(AOB ) PCR-DGGE ACETOCHLOR

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Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils

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