ICC density predicts bacterial overgrowth in a rat model of post-infectious IBS

查看全文 作　　者：Sam-Ryong [1]Jee;Walter [1]Morales;Kimberly [1]Low;Christopher [1]Chang;Amy [1]Zhu;Venkata [1]Pokkunuri;Soumya [1]Chatterjee;Edy [1]Soffer;Jeffrey L [1]Conklin;Mark [1]Pimentel 机构地区：[1]GI Motility Program,Division of Gastroenterology,Cedars-Sinai Medical Center 出　　处：《World Journal of Gastroenterology》索引2010年第16卷第29期,共7页 基　　金：Supported by A grant from Beatrice and Samuel A Seaver Foundation as well as the Shoolman Foundation 摘　　要：AIM:To investigate the interstitial cells of Cajal(ICC) number using a new rat model.METHODS:Sprague-Dawley rats were assigned to two groups.The first group received gavage with Campylobacter jejuni(C.jejuni) 81-176.The second group was gavaged with placebo.Three months after clearance of Campylobacter from the stool,precise segments of duodenum,jejunum,and ileum were ligated in self-contained loops of bowel that were preserved in anaerobic bags.Deep muscular plexus ICC(DMP-ICC) were quantified by two blinded readers assessing the tissue in a random,coded order.The number of ICC per villus was compared among controls,Campylobacter recovered rats without small intestinal bacterial overgrowth(SIBO),and Campylobacter recovered rats with SIBO.RESULTS:Three months after recovery,27% of rats gavaged with C.jejuni had SIBO.The rats with SIBO had a lower number of DMP-ICC than controls in the jejunum and ileum.Additionally there appeared to be a density threshold of 0.12 DMP-ICC/villus that was associated with SIBO.If ileal density of DMP-ICC was < 0.12 ICC/villus,54% of rats had SIBO compared to 9% among ileal sections with > 0.12(P<0.05).If the density of ICC was < 0.12 DMP-ICC/villus in more than one location of the bowel,88% of these had SIBO compared to 6% in those who did not(P<0.001).CONCLUSION:In this post-infectious rat model,the development of SIBO appears to be associated with a reduction in DMP-ICC.Further study of this rat model might help understand the pathophysiology of postinfectious irritable bowel syndrome. 关 键 词：Post-infectious irritable bowel syndrome Bacterial overgrowth Interstitial cells of Cajal CAMPYLOBACTER