Relationship between COX-2 and cell cycle-regulatory proteins in patients with esophageal squamous cell carcinoma

查看全文 作　　者：Jun-Xing [1]Huang; Mao-Song [1]Lin; Yun-Lei [1]Zhang; Feng-Yue [1]Li; Rong-Yu [1]QianWei [2]Xiao;Wei-Chang [3]Chen;Zheng-Xiang [2]Song;Ping [2]Chen;Eeva [4]Salminen 机构地区：[1]Department of Oncology,the People’s Hospital of Taizhou,Taizhou Medical School,Yangzhou and Nantong University,Taizhou 225300,Jiangsu Province,China;[2]Department of Pathology,The People’s Hospital of Taizhou,Taizhou Medical School,Yangzhou and Nantong University,Ta izhou 225300,Jiangsu Province,China;[3]Depar tment of Gastroenterology,The First Affiliated Hospital of Soochow University,Suzhou 215006, Jiangsu Province,China;[4]Department of Radiotherapy and Oncology,Turku University Hospital,Turku,and Finnish Radiation ProtectionAgency (STUK) Finland,POBox 52,20521 Turku,Finland 出　　处：《World Journal of Gastroenterology》索引2010年第16卷第47期,共7页 基　　金：Supported by The '333 Plan' Fund of Jiangsu Province, China, No. 2009-24 摘　　要：AIM: To investigate the correlation between cyclooxygenase-2 (COX-2) and cell cycle-regulatory proteins in patients with esophageal squamous cell carcinoma (ESCC). METHODS: One hundred and two surgically obtained specimens of ESCC were randomly collected. All specimens were obtained from patients who had not received chemoor radiotherapy prior to surgical resection.Twenty-eight specimens of normal squamous epithelium served as controls. The expression of COX-2, Ki-67, cyclin A and p27 was examined by immunohistochemistry. The Pearson test was used to analyze the relationship between groups. RESULTS: The protein level of COX-2, Ki-67 and cyclin A was significantly higher in ESCC than in normal squamous epithelium (74.7±61.2 vs 30.2 ± 43.4, 64.0 ± 51.6 vs 11.6 ± 2.3, 44.2 ± 32.2 vs 11.7 ± 5.0, respectively, all P<0.01). In contrast, the protein level of p27 was signifi cantly lower in ESCC than in normal squamous epithelium (182.0 ±69.0 vs 266.4±28.0, P<0.01). In ESCC, COX-2 expression was correlated with T stage, the score of T1-T2 stage was lower than that of T3-T4 stage (55.0±42.3 vs 83.0 ± 66.5, P<0.05), and Ki-67, cyclin A and p27 expressions were correlated with the tumor differentiation (43.8±31.7 vs 98.4± 84.8, 32.0 ± 19.0 vs 54.1 ±53.7,206.2±61.5 vs 123.5±68.3, respectively, all P<0.01). COX-2 expression was positively correlated to Ki-67, cyclin A and negatively correlated to p27 expression in ESCC (r=0.270, 0.233 and-0.311, respectively, all P<0.05).CONCLUSION: The expression of COX-2 is correlated with tumor cell invasion and is closely related to the cell proliferation in patients with ESCC. 关 键 词：Esophageal squamous cell carcinoma CYCLOOXYGENASE-2 KI-67 Cyclin A P27 Tumor markers IMMUNOHISTOCHEMISTRY