A Rapid and Effective HPLC Method for Identification of Bovis Calculus in Chinese Patent Medicines

查看全文 作　　者：XIA [1]Jing;XIA [2]Li;WANG Wei-[3]yi;SHA Yi-[4]wei;WANG [1]Ke;JI [1]Shen

机构地区：[1]Shanghai Institute for Food and Drug Control;[2]Guangdong Food and Drug Vocational College;[3]Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,State Oceanic Administration (SOA);[4]Shanghai Chongming Institute for Food and Drug Control

出　　处：《Chinese Herbal Medicines》索引2013年第5卷第3期,共11页

摘　　要：Objective Due to the limited resource and the large demand,many kinds of Bovis Calculus(BC)including artificial Bovis Calculus(ABC),in vivo cultured Bovis Calculus(in vivo CBC),and in vitro cultured Bovis Calculus(in vitro CBC)were used in Chinese patent medicines(CPMs).Previous studies have shown that the chemical constituents of ABC and their properties were different from other BC.The two types of CBC with much higher price than ABC were approximately equivalent with natural Bovis Calculus in quality and clinical effect.The aim of the study is to establish a rapid and effective method for the identification of BC in CPMs.Methods An HPLC method with the higher specificity for analyzing bilirubin was established to distinguish ABC from other three kinds of BC by comparing the change of bilirubin content with the addition of EDTA-2Na as the extraction solvent and stabilizer.Results The bilirubin content in CPMs containing ABC was basically unchanged,while that in CPMs containing other kinds of BC showed significant difference.The proposed method was employed to analyze a variety of CPMs containing Bovis Calculus(CPMBCs)and proven to be universal.Conclusion An effective analytical method is established for the quality control of CPMBCs and further ensures the safety and efficacy of these drugs in clinical practice. 关键词：artificial BOVIS CALCULUS BILIRUBIN BOVIS CALCULUS Chinese PATENT MEDICINES CULTURED BOVIS CALCULUS EDTA-2Na solution

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A Rapid and Effective HPLC Method for Identification of Bovis Calculus in Chinese Patent Medicines

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