Smashing Tissue Extraction and HPLC Determination of Paclitaxel and 10-Deacetylbaccatin from Taxus x media

查看全文 作　　者：LIU Yan-ze1,2,GAO Wen-qin3,WANG Ji-wen4,ZHANG Yu3,ZHAO Yu-qing4,5 1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China 2.Bio-Organic and Natural Product Laboratory,McLean Hospital/Harvard Medical School,MA 02478,USA 3.Shenyang Pharmaceutical University,Shenyang 110006,China 4.Shenyang Tianfeng Bioengineer Technology Co.,Ltd.,Shenyang 110015,China 5.Key Laboratory of Structure-Based Drug Design & Discovery,Ministry of Education,Shenyang Pharmaceutical University,Shenyang 110016,China

出　　处：《Chinese Herbal Medicines》索引2011年第3卷第3期,共4页

基　　金：E&T modern center for Natural Products of Liaoning Province of China (2008402021) 摘　　要：Objective To optimize the extraction technology of Taxus x media by using the contents of Paclitaxel and 10-deacetylbaccatin(10-DAB) ,two representative active diterpene alkaloids of taxane type from T.x media,as evaluation standard.Methods The smashing tissue extraction(STE) of Paclitaxel and 10-DAB from T.x media,was investigated by comparing with ultrasonic extraction(UE) which was one of the modern technologies of extraction.Results STE was more efficient than UE,and the contents of 10-DAB and Paclitaxel in the extracts obtained by STE were higher than those by UE.Conclusion STE is a fast,high-performance,and energy-saving technology for the extraction of diterpene alkaloids of taxane type.STE also provides a simple,component-safe,workable,and highly efficient method for the extraction of active natural product. 关键词：10-deacetylbaccatin HPLC PACLITAXEL smashing tissue extraction Taxus x media

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Smashing Tissue Extraction and HPLC Determination of Paclitaxel and 10-Deacetylbaccatin from Taxus x media

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