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Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) supports adhesion and migration of mesenchymal stem cells and tenocytes

查看全文 作　　者：Alex J [1]Lomas;George GQ [2]Chen;Alicia J El [1]Haj;Nicholas R [1]Forsyth 高影响力作者 机构地区：[1]Guy Hilton Research Centre, Keele University, Stoke on Trent, ST4 7QB, United Kingdom;[2]Department Biological Sciences and Biotechnology, School of Life Science, Tsinghua University高影响力机构 出　　处：《World Journal of Stem Cells》索引2012年第4卷第9期,共7页高影响力期刊 基　　金：Supported by EPSRC Doctoral Training Centre in Regenerative Medicine and the HYANJI Scaffold Project (European Commission Framework 7 program) 摘　　要：AIM: To establish the potential of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) as a material for tendon repair. METHODS: The biocompatibility of PHBHHx with both rat tenocytes (rT) and human mesenchymal stem cells (hMSC) was explored by monitoring adhesive characteristics on films of varying weight/volume ratios coupled to a culture atmosphere of either 21% O2 (air) or 2% O2 (physiological normoxia). The diameter and stiffness of PHBHHx films was established using optical coherence tomography and mechanical testing, respectively. RESULTS: Film thickness correlated directly with weight/volume PHBHHx (r2 = 0.9473) ranging from 0.1 mm (0.8% weight/volume) to 0.19 mm (2.4% weight/volume). Film stiffness on the other hand displayed a biphasic response which increased rapidly at values > 1.6% weight/volume. Optimal cell attachment of rT required films of ≥ 1.6% and ≥ 2.0% weight/volume PHBHHx in 2% O2 and 21% O2 respectively. A qualitative adhesion increase was noted for hMSC in films ≥ 1.2% weight/volume, becoming significant at 2% weight/volume in 2% O2. An increase in cell adhesion was also noted with ≥ 2% weight/volume PHBHHx in 21% O2. Cell migration into films was not observed. CONCLUSION: This evaluation demonstrates that PHBHHx is a suitable polymer for future cell/polymer replacement strategies in tendon repair. 关 键 词：MESENCHYMAL stem cell TENOCYTES POLYHYDROXYALKANOATES Hypoxia Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
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