Anti-mycobacterial screening of five Indian medicinal plants and partial purification of active extracts of Cassia sophera and Urtica dioica

查看全文 作　　者：Rambir [1]Singh;Shariq [2]Hussain;Rajesh [3]Verma;Poonam [2]Sharma 机构地区：[1]Department of Biomedical Sciences,Bundelkhand University,Kanpur Road,Jhansi-284128,India;[2]Department of Zoology,Bundelkhand University,Kanpur Road,Jhansi-284128,India;[3]Department of Microbiology,Maharani Laxmi Bai Medical College,Jhansi,India 出　　处：《Asian Pacific Journal of Tropical Medicine》索引2013年第6卷第5期,共6页 摘　　要：Objective:To find out the anti-mycobacterial potential of Cassia sophera(C.sophera),Urtica dioica(U.dioica),Momordica dioica,Tribulus terrains and Coccinia indica plants against multidrug resistant(MDR) strain of Mycobacterium tuberculosis(M.tuberculosis).Methods:Plant materials were extracted successively with solvents of increasing polarity.Solvent extracts were screened for anti-mycobacterial activity against fast growing,non-pathogenic mycobacterium strain,Mycobacterium semegmatis,by disk diffusion method.The active extracts were tested against MDR and clinical isolates of M.tuberculosis by absolute concentration and proportion methods.The active extracts were subjected to bio-autoassay on TLC followed by silica column chromatography for isolation of potential drug leads.Results:Hexane extract of U.dioica(HEUD) and methanol extract of C.sophera(MECS) produced inhibition zone of 20 mm in disc diffusion assay and MIC of 250 and 125μg/mL respectively in broth dilution assay against Mycobacterium semegmatis.Semipurified traction F2 from MECS produced 86%inhibition against clinical isolate and 60%inhibition against MDR strain of ilf.tuberculosis.F18 from HEUD produced 81%inhibition against clinical isolate and 60%inhibition against MDR strain of M.tuberculosis.Phytochemical analysis indicated that anti-mycobacterial activity of MECS may be due to presence of alkaloids or flavonoids and that of HEUD due to terpenoids.Condosions:C.sophera and U.dioica plant extracts exhibited promising anti-mycobacterial activity against MDR strain of M.tuberculosis. This is the first report of anti-mycobacterial activity form C.sophera.This study showed possibility of purifying novel anti-mycobacterial compound(s) from C.sophera and U.dioica. 关 键 词：Tuberculosis In VITRO study MEDICINAL plants Minimum INHIBITORY concentration