Antioxidant and cytotoxic effects of hexane extract of Morinda pubescens leaves in human liver cancer cell line

查看全文 作　　者：Jaya Kumar [1]D;Jaya Santhi [1]R

机构地区：[1]P.G & Research Department of Chemistry,Auxilium College,Vellore-632 006,Tamil Nadu,India

出　　处：《Asian Pacific Journal of Tropical Medicine》索引2012年第5卷第5期,共5页

基　　金：UGC,New Delhi,India for their financial support 摘　　要：Objective:To evaluate the antioxidant and cytotoxic effects of hexane extract of Morinda pubescens leaves and to find the primary bioactive compound responsible for antioxidant and cytotoxic activities.Methods:The individual compounds were isolated using column chromatography and were characterized by spectroscopic techniques.The antioxidant activity was evaluated for all individual isolated compounds by DPPH method using L-Ascorbic acid as standard and cytotoxicity was assessed for the extract and the hyoscyamine by MTT assay, caspase test and RT-PCR study.Results:The antioxidant activity of the isolated compounds and the extract increased as the concentration increased.One of the isolated compound hyoscyamine showed the high antioxidant activity.The extract and the hyoscyamine dose-dependently decreased the cell viability in HepG2 cells.Hyoscyamine induced caspase-3 mediated apoptosis. Up regulation of p53 gene expression provides cue for apoptotic activity of hyoscyamine. Conclusions:The results indicate that hexane extract possessed potent antioxidant and cytotoxic activity and hyoscyamine is the principal bioactive compound in hexane extract. 关键词：DPPH MORINDA pubescens ANTIOXIDANT HepG2 Caspase MTT assay

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Antioxidant and cytotoxic effects of hexane extract of Morinda pubescens leaves in human liver cancer cell line

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耦合文献(13)

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