Multilocus enzyme electrophoresis analysis of Echinostoma revolutum and Echinostoma malayanum(Trematoda:Echinostomatidae) isolated from Khon Kaen Province,Thailand

查看全文 作　　者：Weerachai [1]Saijuntha;Sumonta [2]Tapdara;Chairat [3]Tantrawatpan 机构地区：[1]Walai Rukhavej Botanical Research Institute(WRBRI),Mahasarakham University,Mahasarakham,Thailand;[2]Clinical Pathology Laboratory,Amnatcharoen hospital,Amnatcharoen,Thailand;[3]Division of Cell Biology,Department of Preclinical Sciences,Faculty of Medicine,Thammasat University,Rangsit Campus,Pathumthani,Thailand 出　　处：《Asian Pacific Journal of Tropical Medicine》索引2010年第3卷第8期,共4页 基　　金：supported by the Thailand Research Fund and the Commission on Higher Education(grant no. MRG5180102 to Weerachai Saijuntha). 摘　　要：Objective:To explore the genetic variation and differentiation of 2 echinostomes from genus Echinostoma,i.e.Echinostoma revolutum(E.revolution) and Echinostoma malayanum(E. malayanum) from Khon Kaen Province,Thailand.Methods:These parasites were compared at 22 enzymes encoding a presumptive 30 loci by using multilocus enzyme electrophoresis (MEE) technique.Results:Twenty-two loci can be used as diagnostic markers to differentiate these 2 species.E.revolutum and E.malayanum had fixed genetic differences at 70%of loci, whereas both species had fixed genetic differences from the liver fluke,Opisthorchis viverrini at 91%of loci.Intraspecific variation within a population of E.revolutum was observed at 5 polymorphic loci.Conclusions:MEE is a powerful technique to investigate genetic variation and differentiation of E.revolutum and E.malayanum. 关 键 词：Echinostoma Echinostomes GENETIC variation MULTILOCUS ENZYME ELECTROPHORESIS ALLOZYME