Phenotypic methods of greater accuracy to detect the mecA gene product for the recognition of MRSA in resource constraint settings

查看全文 作　　者：Rasheed [1]MU;Ahmed [2]Z

机构地区：[1]Assistant Professor Department of Medical Microbiology Haramaya University,Harar,Ethiopia;[2]Head-School of Medical Laboratory Technology Jimma University,Jimma,Ethiopia

出　　处：《Asian Pacific Journal of Tropical Medicine》索引2010年第3卷第9期,共4页

摘　　要：Objective:To evaluate the detection of methicillin resistant Staphylococcus aureus(MRSA) and analyze the performance of Mastalex MRSA(Mast,UK).Methods:Two hundred and ten Staphylococcus aureus(S.aureus) strains were isolated from different clinical samples and were tested for methicillin resistance by Oxacillin(1μg) and Cefoxitin(30μg) disc diffusion, oxacillin agar screen,and minimum inhibitory concentration of oxacillin and cefoxitin.S.aureus isolates were grown on the blood agar and mannitol salt agar with(2 mg/L) and without oxacillin for the analysis of Mastalex MRSA.Results:Out of 210 S.aureus strains tested,103 strains were detected as methicillin resistant by Cefoxitin disk diffusion,Cefoxitin minimal inhibitory concentration(MlC) and Mastalex MRSA test.Whereas oxacillin disc diffusion and oxacillin agar screen detected 91 and 97 MRSA respectively.The Cefoxitin MIC test performance was equivalent to Cefoxitin disc diffusion.103(100%) strains grown on blood agar without and with oxacillin,and 76(74%) and 93(91%) strains grown on mannitol salt agar without and with oxacillin shown positive agglutination with Mastalex MRSA test respectively.Conclusions:The cefoxitin disk diffusion/ Mastalex MRSA is very suitable for detection of MRSA and the tests can be an alternative to PCR for detection of MRSA in resource constraint settings.Mastalex test would be particularly useful when confirmation of resistance is urgently required. 关键词：STAPHYLOCOCCUS AUREUS OXACILLIN Cefoxitin Mastalex MRSA

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Phenotypic methods of greater accuracy to detect the mecA gene product for the recognition of MRSA in resource constraint settings

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