Green synthesis,antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract

查看全文 作　　者：Ghassan Mohammad [1]Sulaiman;Wasnaa Hatif [1]Mohammed;Thorria Radam [1]Marzoog;Ahmed Abdul Amir Al-[2]Amiery;Abdul Amir [3]H.Kadhum;Abu Bakar Mohamad

机构地区：[1]Biotechnology Division,Applied Science Department,University of Technology;[2]Applied Chemistry Division,Applied Science Department,University of Technology;[3]Chemical and Process Engineering Department,Universiti Kebangsaan Malaysia

出　　处：《Asian Pacific Journal of Tropical Biomedicine》索引2013年第3卷第1期,共6页

基　　金：Supported by University of Technology.Baghdad.Iraq and Universiti Kebangsaan Malaysia(Grant No.DIP-2012-02) 摘　　要：Objective:To synthesize silver nanopaticles from leaves extract of Eucalyptus chapmaniana(E.chapmaniana)and test the antimicrobial of the nanoparticles against different pathogenic bacteria,yeast and its toxicity against human acute promyelocytic leukemia(HL-60)cell line.Methods:Ten milliliter of leaves extract was mixed with 90 mL of 0.01 mmol/mL or 0.02 mmol/mL aqueous AgNO_3 and exposed to sun light for 1 h.A change from yellowish to reddish brown color was observed.Characterization using UV-vis spectrophotometery and X-ray diffraction analysis were performed.Antimicrobial activity against six microorganisms was tested using well diffusion method and cytoxicity test using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,a yellow tetrazole was obtained on the human leukemia cell line(HL-60).Results:UV-vis spectral analysis showed silver surface plasmon resonance band at 413 nm.X-ray diffraction showed that the particles were crystalline in nature with face centered cubic structure of the bulk silver with broad beaks at 38.50°and 44.76°.The synthesized silver nanoparticles efficiently inhibited various pathogenic organisms and reduced viability of the HL-60 cells in a dose-dependent manner.Conclusions:It has been demonstrated that the extract of E.chapmaniana leaves are capable of producing silver nanoparticles extracellularly and the Ag nanoparticles are quite stable in solution.Further studies are needed to fully characterize the toxicity and the mechanisms involved with the antimicrobial and anticancer activity of these particles. 关键词：EUCALYPTUS chapmaniana Silver nanoparticles ANTIMICROBIAL activity CYTOTOXIC effect

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Green synthesis,antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract

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