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Infection of Schistosomiasis japanicum is likely to enhance proliferation and migration of human breast cancer cells:mechanism of action of differential expression of MMP2 and MMP9

查看全文 作　　者：Ya-Ling [1]Lin;Rakesh [2]Ramanujum;Shiping [2]He 高影响力作者 机构地区：[1]Department of Parasitology,China Medical University School of Medicine;[2]Department of Biological Sciences,National Sun Yat-sen University高影响力机构 出　　处：《Asian Pacific Journal of Tropical Biomedicine》索引2011年第1卷第1期,共6页高影响力期刊 基　　金：Supported by grants from National Science Council(NSC98-2314-B-110-001-MY3) 摘　　要：Objective:To study whether the infection of Schistosomiasis japanicum(S.japanicum) is related to enhanced proliferation and migration of cancer cells,and the molecular mechanism pertains to cancer cell metastasis in human host.Methods:The gene of S.japanicum glutathione transferase(sjGST) cloned from 5.japanicum was expressed,purified and applied in a series of assays to explore the effect of sjGST on proliferation and migration of MDA-MB-435S,and the expression of MMP2 and MMP9.Immunofluorescence assay for the binding of sjGST to MDA-MB-435S was also carried out.Results:Results showed that sjGST enhanced proliferation and migration in human breast cancer cell MDA-MB-435S signifycantly at 50-200 nM,but did not enhance them in human lung cancer cell A549.Immunofluorescence assay for the binding of sjGST to MDA-MB-435S and A549 showed that GST was readily hound to the breast cancer cells,but showed almost no binding to human lung cancer cells.The assays for gelatinase activity showed that both MMP2 and MMP9 activities were increased significantly in the presence of sjGST(50-200 nM) in MDA-MB-435S, but they were not significant in A549.Conclusions:Our current results show strongly that S. japanicum GST binds to MDA-MB-435S probably via its i'eceptor,and enhances proliferation and migration of the cancer cells by up-regulatory expression of MMP2 and MMP9. 关 键 词：INFECTION SCHISTOSOMIASIS japanicum Glutathione s-transferase PROLIFERATION Gelatinase MMP2 and MMP9 MIGRATION Breast cancer
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