PLLA-PCys co-electrospun fibers for capture and elution of glutathione S-transferase

查看全文 作　　者：LU [1,2,3]TianCheng;SUN [2,3]Jing;DONG [2,3]XiaoQing;CHEN [2]XueSi;WANG [2]Yu;JING [2]XiaBin

机构地区：[1]Jilin Agricultural University,Institute of Life Science,Changchun 130118,China;[2]State Key Laboratory of Polymer Physics and Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;[3]Graduate University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Science China Chemistry》索引2009年第52卷第12期,共5页

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 20674084 and 50425309) 摘　　要：The copolymer poly(L-lactic acid)-b-poly(L-cysteine) (PLA-b-PCys) was co-electrospun with PLGA into ultrafine fibers. The reduced glutathione (GSH) was conjugated to the fiber surfaces via disulfide bonds. The glutathione S-transferase (GST) was captured onto the GSH fibers via specific substrate-enzyme interaction between the bound GSH and GST. The captured GST was eluted with free GSH aqueous solution and lyophilized to get pure GST powders. The results show that the GSH moieties on the fiber surface retain the bioactivity of the free GSH and thus they can bind specifically with GST and the GST in solution is captured onto the fiber surface. In addition, the bound GSH is not as active as free GSH so that the captured GST can be eluted off from the fiber by free GSH aqueous solution. Based on this principle, GST itself or its fused proteins can be separated and purified very easily. The preliminary purification efficiency is 6.5 mg·(gPCys)-1. Further improvements are undertaken. 关键词：ELECTROSPUN fibers GLUTATHIONE GLUTATHIONE S-TRANSFERASE protein separation and purification

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PLLA-PCys co-electrospun fibers for capture and elution of glutathione S-transferase

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