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Characterization of interspecific hybrids and backcross progenies from a cross between Oryza minuta and Oryza sativa

查看全文 作  者:GUO SiBin, QIN FaLan, ZHANG DuanPin & LIN XingHua· National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070, China 高影响力作者 出  处:《Science China(Life Sciences)》索引2009年第52卷第12期,共8页高影响力期刊 基  金:Supported by the National Natural Science Foundation of China (Grant No. 30370 869) 摘  要:Oryza minuta, a tetraploid wild relative of cultivated rice, is an important source for the genetic improvement. Interspecific hybrids were obtained from the cross of O. sativa L. (IR24) and O. minuta (Acc. No. 101133) with 5.58% crossability, which ranged from 0.11% to 1.62% in the backcross generations. The chromosome numbers of the backcross progenies were 24 to 48. Seven yield-related traits of the parents, hybrid F1, and backcross progenies were evaluated. Simple sequence repeat markers analysis showed that the polymorphism ratio of SSR bands between IR24 and Acc. No. 101133 was 93.2%. The average donor segment number, length, donor genome size, and percentage of donor genome of 92 BC3F1 plants (2n=24) were 24.1, 17.8 cM, 438.4 cM and 26.2%, respectively. They were complex variation and uneven among the chromosomes. These introgression lines could be used to identify the favorable genes of O. minuta and provide a new platform for the genetic improvement of cultivated rice. 关 键 词:ORYZA sativa L. ORYZA minuta INTERSPECIFIC hybridization BACKCROSS PROGENY INTROGRESSION line
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