Assessment of candidate plant DNA barcodes using the Rutaceae family

查看全文 作　　者：LUO Kun1,2,CHEN ShiLin1,CHEN KeLi2,SONG JingYuan1,YAO Hui1,MA XinYe1,ZHU YingJie3,PANG XiaoHui1,YU Hua1,LI XiWen1,4 & LIU Zhen2 1Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100193,China;2College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430061,China;3School of Bioscience and Engineering,Southwest Jiaotong University,Chengdu 610031,China;4Department of Chemistry,Tsinghua University,Beijing 100084,China

出　　处：《Science China(Life Sciences)》索引2010年第53卷第6期,共8页

基　　金：supported by the National International Cooperation Program (Grant No. 2007DFA30990);Special Scientific Research Project of Health Profession (Grant No. 200802043) 摘　　要：DNA barcoding is a rapidly developing frontier technology that is gaining worldwide attention.Here,seven regions (psbA-trnH,matK,ycf5,rpoC1,rbcL,ITS2,and ITS) with potential for use as DNA barcodes were tested for their ability to identify 300 samples of 192 species from 72 genera of the family Rutaceae.To evaluate each barcode’s utility for species authentication,PCR amplification efficiency,genetic divergence,and barcoding gaps were assessed.We found that the ITS2 region exhibited the highest inter-specific divergence,and that this was significantly higher than the intra-specific variation in the 'DNA barcoding gap' assessment and Wilcoxon two-sample tests.The ITS2 locus had the highest identification efficiency among all tested regions.In a previous study,we found that ITS2 was able to discriminate a wide range of plant taxa,and here we confirmed that ITS2 was also able to discriminate a number of closely related species.Therefore,we propose that ITS2 is a promising candidate barcode for plant species identification. 关键词：DNA BARCODING ITS2 RUTACEAE identification

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Assessment of candidate plant DNA barcodes using the Rutaceae family

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