Protective effects ofLactobacillus plantarum against epithelial barrier dysfunction of human colon cell line NCM460

查看全文 作　　者：Mary Pat [1]Moyer

机构地区：[1]INCELL Corporation,San Antonio,TX 78249,United States

出　　处：《World Journal of Gastroenterology》索引2010年第16卷第45期,共7页

基　　金：Supported by The National Natural Science Foundation of China,No.30672044;the Ministry of Science and Technology of China,No.2008CB517403 摘　　要：AIM: To investigate the effects of Lactobacillus plantarum (L. plantarum ) in the intestinal permeability and expression of tight junction (TJ) using the normal human colon cell line NCM460. METHODS: Paracellular permeability of NCM460 monolayers was determined by transepithelial electrical resistance and dextran permeability. Expression of TJ proteins in NCM460 cell monolayers was detected by Western blotting and quantitative real-time polymerase chain reaction.RESULTS: L. plantarum played an important role in increasing transepithelial electrical resistance and decreasing the permeability to macromolecules of NCM460 monolayers against the disruption caused by enteropathogenic Escherichia coli (E. coli ) or enteroinvasive E. coli . L. plantarum also prevented the decrease in the expression of TJ proteins and F-actin in NCM460 cells.CONCLUSION: L. plantarum can protect against dysfunction of NCM460 intestinal epithelial barrier caused by enteropathogenic E. coli or enteroinvasive E. coli , and thus can be a potential candidate of therapeutic agents for the treatment of intestinal diseases. 关键词：LACTOBACILLUS PLANTARUM NCM460 TIGHT JUNCTION Intestinal barrier

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Protective effects ofLactobacillus plantarum against epithelial barrier dysfunction of human colon cell line NCM460

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