cAMP receptor protein (CRP) downregulates Klebsiella pneumoniae nif promoters in Escherichia coli

查看全文 作　　者：Zhiting [1]Li;Yicheng [1]Sun;Xianjun [1]Mao;Yiping [1]Wang

机构地区：[1]Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China

出　　处：《Chinese Science Bulletin》索引2002年第47卷第19期,共7页

基　　金：This work was supported in part by the NationalNatural Science Foundation of China (Grant No. 39925017);the State '863' Program (Grant No. 2001AA214021);the '973' Program (Grant No. 2001CB108900) from the Ministry of Science and Technology of China. 摘　　要：In enteric bacteria, in response to the PTS system, the cAMP receptor protein (CRP) mediates the glucose effect, via regulating σ70-dependent catabolic genes at transcriptional level. In this study, it is observed that the nitrogen fixation capacity of Klebsiella pneumoniae varies strongly when cells are grown on different carbohydrates, and this carbon effect occurs at the level of nif gene expression. Here we show that CRP can repress σ54-dependent nif promoters (nifB, nifE, nifF, nifH, nifJ, nifLA and nifU), in a cAMP dependent fashion, in closed related E. coll background. Sequence analysis of these nif promoters indicates that there is no direct correlation between the fold of CRP-cAMP-mediated inhibition and the upstream cis elements at the promoters. In addition, the crp gene of K. pneumoniae has been isolated and sequenced, which is structural and functional highly homologous to that of E. coli. This suggests that CRP-cAMP-mediated inhibition on the nif promoters could be the reason for 关键词：CRP-cAMP nif PROMOTER REGULATION of gene expression NITROGEN fixation.

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cAMP receptor protein (CRP) downregulates Klebsiella pneumoniae nif promoters in Escherichia coli

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