Anaerobic bacteria and intrahepatic stones: detections of Clostridium sp. and Bacteroides fragilis

查看全文 作　　者：LIU [1]Yanlei;Kan [1]Lam;Yajarayma J [1]Tang;Paul H [1]Gumerlock;Dong Ki [1]Lee;Myung Hwa [1]Kim;Sum P Lee Joseph [1]SilvaJr;Joseph W [1]Leung

机构地区：[1]Division of Gastroenterology and Mr & MrsCWLaw Biofilm Research Center,University of California,Davis MedicalCenter,Sacramento,CA95817,USA

出　　处：《Chinese Medical Journal》索引2000年第9期,共4页

基　　金：ThisworkwassupportedbyVAMeritReviewGrant(No 940 70 0 0 3) 摘　　要：To detect anaerobic bacteria Clostridium sp . and Bacteroides fragilis in intrahepatic stones by molecular genetic method Methods DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp . and the glutamine synthetase gene of Bacteroides fragilis Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp Results 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83%, 49/59) The two most common groups were detected in 19 (41%) and 17 (37%) of the 46 samples using SSPC analysis, and 25/59 (42%) stones were tested positive for Bacteroides fragilis Conclusions Anaerobes such as Clostridium sp and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation PCR is a useful technique to detect fastidious pathogens, which are difficult to culture SSCP of PCR products is a rapid method in differentiating bacterial 关键词：INTRAHEPATIC STONE · ANAEROBIC bacteria · PCR · SINGLE-STRAND confromational POLYMORPHISM

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Anaerobic bacteria and intrahepatic stones: detections of Clostridium sp. and Bacteroides fragilis

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