Immunohistochemical Studies of Macrophages and MHC Class Ⅱ -positive Cells in the Iris and Ciliary Body of Lewis Rats

查看全文 作　　者：Alex F.de [1]Vos;Aize [1]Kijlstra 机构地区：[1]Netherlands Ophthalmic Research Institue,Netherlands Ophthalmic Research Institue University of Amsterdam,Amsterdam,The Netherlands 出　　处：《Eye Science》索引1996年第16卷第1期,共5页 基　　金：a grant from Pubic Health Ministry of China;Natural Science Fundation of Guangdong Province 摘　　要：Purpose:To investigate the density, distribution and morphology of macrophages and MHC class II -positive dendritic cells in the iris and ciliary body of lewis rats. Methods:Immunohistochemistry was performed using monoclonal antibodies specific to monocytes and macrophages (ED1,ED2) and MHC class II -positive cells (OX6) on wholemounts of the iris-ciliary body complex isolated form normal lewis rats.Results:A well developed network of macrophages was present in the iris and ciliary body of normal lewis rats. These cells, morphologically displaying dendriti-form or pleiomorphic appearance, were more densely arranged in mid-iris (950 + 189 cells/mm2) than in iris base (482 ± 78 cells/mm2) and pupil margin (595 ± 92 cells/mm2). A similar network of MHC class II -positive cells with a cell density 452 ± 78 cells/mm2 was almost uniformly distributed in the iris of normal lewis rats.Conclusions : A network of macrophages and MHC class II -positive cells was established in the iris and ciliary body of 关 键 词：wholemounts of iris-ciliary body MACROPHAGES MHC CLASS Ⅱ-positive cells immunohistochemistry