维普中文期刊产品整合服务

Apoptosis in Granulosa cells during follicular atresia:relationship with steroids and insulin-like growth factors

查看全文 作　　者：YuanSongYU HongShuSUI ZhengBinHAN WeiLI MingJiuLUO [1]JingHeTAN 高影响力作者 机构地区：[1]LaboratoryofAnimalReproductionandEmbryologyCollegeofAnimalSciencesandVeterinaryMedicine,ShandongAgriculturalUniv.ersity,Taian,Shandong271018,China高影响力机构 出　　处：《Cell Research》索引2004年第14卷第4期,共6页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China(No.30070556);the Major State Basic Research Development Program of China(No.G200016107). 摘　　要：It is well known that during mammalian ovarian follicular development, the majority of follicles undergo atresia at various stages of their development. However, the mechanisms controlling this selection process remain unknown. In this study, we investigated apoptosis in granulosa cells during goat follicular atresia by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The changes in the levels of steroids, insulin-like growth factors (IGFs) and IGF receptors were studied by radioimmunoassay (RIA) and semi-quantitative reverse transcription-PCR. We found that the percentage of apoptotic granulosa cells in the atretic (A) follicles was significantly higher than that in the slightly atretic (SA) and healthy (H) follicles. The level of estradiol and the ratio of estradiol to progesterone in H follicles were significantly higher than those in A follicles. On the other hand, the level of progesterone was not significantly different among these follicle types. We also found that the level of IGF-I in H follicles was higher than in SA and A follicles, whereas the amount of IGF-Ⅱ did not vary significantly. The expression of IGF receptor also decreased in A follicles as compared to that in H and SA follicles. These results suggested that estradiol and IGF-I might be involved in controlling apoptosis in granulosa cells during follicular atresia. 关 键 词：肉芽瘤细胞 囊泡闭锁 细胞凋亡 类固醇 胰岛素样生长因子 卵巢
相关文献

参考文献(11)

引证文献(25)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费