Expression of survivin in primary and metastatic gastric cancer cells obtained by laser capture microdissection

查看全文 作　　者：Zhen-[1]NingWang;Hui-[2]MianXu;[1]LiJiang;[2]XinZhou;[2]ChongLu;[1]XueZhang

机构地区：[1]TheResearchCenterforMedicalGenomicsandMOHKeyLaboratoryofCellBiology,ChinaMedicalUniversity,Shenyang110001,LiaoningProvince,China;[2]DepartmentofSurgicalOncology,theFirstAffiliatedHospitalofChinaMedicalUniversity,Shenyang110001,LiaoningProvince,China

出　　处：《World Journal of Gastroenterology》索引2004年第10卷第21期,共5页

基　　金：Supported by the Nationa 1973 Program of China,No.G 1998051203,the National Science Fund for Distinguished Young Scholars of China,No.30125017,and the MOE TRAPOYT Program of China,No.1999-96 摘　　要：AIM:Survivin, a recently identified member of the inhibitor of apoptosis protein family, is expressed during development and in various human cancers. However, its expression in normal tissues and clinical relevance in cancers are still debated. In the present study, we analyzed the expression of the survivin gene in human primary and metastatic gastric cancer cells as well as in paired epithelial cells from normal gastric mucosa by means of a novel laser capture microdissection (LCM) technique coupled with reverse transcription -polymerase chain reaction (RT-PCR).METHODS: Thirty patients who had undergone gastrectomy with lymph node dissection for gastric cancer without preoperative treatments were included. Neoplastic tissue, metastatic lymph nodes, and apparently uninvolved normal tissue were collected from each patient. LCM-captured 'pure' cell groups were respectively subjected to RT-PCR analysis with primers specific for the survivin gene.RESULTS: Of the paired samples from 30 gastric cancer patients studied, 24 (80%) primary gastric cancer cell groups and 7 (23%) adjacent morphologically 'normal' gastric epithelial cell groups were shown to have a detectable survivin expression. There was a statistically significant difference in suvivin expression between these two groups (P<0.01). Meanwhile, 95% (19/20) of the metastatic gastric cancer cell groups from lymph nodes had a clear expression of the survivin gene. However, no significant correlation between survivin expression and clinicopathological features of gastric cancer was observed in the present study. CONCLUSION: Survivin expression is present in the majority of gastric cancer cell groups obtained by LCM techniques. The high expression rate in metastatic lesions suggests a possible role of survivin in cancer invasiveness and metastasis. It may contribute to the detection of gastric cancer micrometastasis as a potential molecular marker. In addition, the high expression percentage renders survivin a potential target in the therapy for gastric cancer. 关键词：基因表达初级转移性胃癌细胞肿瘤激光显微解剖

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Expression of survivin in primary and metastatic gastric cancer cells obtained by laser capture microdissection

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