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Purification and Characterization of Jararassin-I, A Thrombin-like Enzyme from Bothrops jararaca Snake Venom

查看全文 作　　者：Débora F. [1,2,3]VIEIRA;Leandra [1,2,3]WATANABE;Carolina D. SANT'[1,2,3]ANA;Silvana [1,2,3]MARCUSSI;Suely V. [1,2,3]SAMPAIO;Andreimar M. [1,2,3]SOARES;Raghuvir K. [1,2,3]ARNI 高影响力作者 机构地区：[1]Departamento de Física, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, UNESP, 15054-000, Sāo José do Rio Preto, SP, Brazil,Departamento de Física, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, UNESP, 15054-000, Sāo José do Rio Preto, SP, Brazil,Unidade de Biotecnologia, Universidade de Ribeirāo Preto, UNAERP, Ribeirāo Preto, SP, Brazil;[2] Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, FCFRP, Universidade de S?o Paulo, USP, Ribeirāo Preto, SP, Brazil,Unidade de Biotecnologia, Universidade de Ribeirāo Preto, UNAERP, Ribeirāo Preto, SP, Brazil;[3] Departamento de Bioquímica e Imunologia, FMRP, Universidade de S?o Paulo, USP, Ribeirāo Preto, SP, Brazil,Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, FCFRP, Universidade de Sāo Paulo, USP, Ribeirāo Preto, SP, Brazil,Unidade de Biotecnologia, Universidade de Ribeirāo Preto, UNAERP, Ribeirāo Preto, SP, Brazil,Departamento de Física, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, UNESP, 15054-000, Sāo José do Rio Preto, SP, Brazil高影响力机构 出　　处：《Acta Biochimica et Biophysica Sinica》索引2004年第36卷第12期,共5页高影响力期刊 摘　　要：A thrombin-like serine protease, jararassin-I, was isolated from the venom of Bothrops jararaca. The protein was obtained in high yield and purity by a single chromatographic step using the affinity resin Benzamidine-Sepharose CL-6B. SDS-PAGE and dynamic light scattering analyses indicated that the molecular mass of the enzyme was about 30 kD. The enzyme possessed fibrinogenolytic and coagulant activities. The jararassin-I degraded the Bβ chain of fibrinogen while theAα chain and γ chain were unchanged. Proteases inhibitors, PMSF and benzamidine inhibited the coagulant activity. These results showed jararassin- I is a serine protease similar to coagulating thrombin-like snake venom proteases, but it specifically cleaves Bβ chain of bovine fibrinogen. Single crystals of enzyme were obtained (0.2 mm×0.2 mm×0.2 mm) and used for X-ray diffraction experiments. 关 键 词：丝氨酸蛋白酶 凝血酶类似物 巴西具窍蝮蛇 蛇毒 纯化 纤维蛋白原溶解活性 结晶
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