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Differences in platelet endothelial cell adhesion molecule-1 expression between peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis

查看全文 作　　者：Hong-[1]KaiGao;Zong-[1]GuangZhou;Fang-[2]HaiHan;You-[3]QinChert;Wen-[1]WeiYan;[1]TaoHe;[1]CunWang;[1]ZhaoWang 高影响力作者 机构地区：[1]DepartmentofGeneralSurgery&DivisionofDigestiveSurgeryandOrgan-Microcirculation,WestChinaHospital,SichuanUniversity,Chengdu610041,SichuanProvince,China;[2]DepartmentofGastrointestinalSurgery,FirstAffiliatedHospital,ZhongshanUniversity,Guangzhou510120,GuangdongProvince,China;[3]CardiovascularBiology,OklahomaMedicalResearchFoundation,OklahomaCity,OK73104,USA高影响力机构 出　　处：《World Journal of Gastroenterology》索引2005年第11卷第5期,共4页高影响力期刊 基　　金：Supported by National Natural Science Foundation of China, No.39925032 摘　　要：AIM: To investigate the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonuclear leukocytes (PMNs) in peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis (AEP).METHODS: Fifty Wistar rats were randomly divided into control group (n=10) and AEP group (n=40). A model of AEP was established by subcutaneous injection of cerulein 5.5 and 7.5 μg/kg at 0 and 1 h after the beginning of experiment respectively. PECAM-1 expression on PMNs from splenic vein and inferior vena cava was determined by RT-PCR at mRNA level and determined by flow cytometry at protein level.RESULTS: In experimental rats, an increased PECAM-1mRNA expression was seen from 4 to 8 h of AEP in peripheral circulation (0.77±0.25%, 0.76±0.28%, 0.89±0.30%,1.00±0.21% ), while in pancreatic microcirculation,expression decreased from 2 h and reached the lowest level at 6 h of AEP (0.78±0.29%, 0.75±0.26%, 0.62±0.28%,0.66±0.20%). There were significant differences at 8-h time point of AEP between peripheral circulation and pancreatic microcirculation (1.00±0.21% vs0.66±0.20%, P<0.05).Meanwhile,the difference at protein level was also found.CONCLUSION: A reverse expression of PECAM-1 on PMNs was found between peripheral circulation and pancreatic microcirculation, suggesting that inhibition of PECAM-1expression may improve the pathological change of AEP. 关 键 词：血小板 内皮细胞 支持分子-1 基因表达 外周循环 胰腺微循环 蛙皮缩胆囊肽 诱导作用 消化系统 急性胰腺炎水肿
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