Genome-wide identification of R genes and exploitation of candidate RGA markers in rice

查看全文 作　　者：[1]WANGXusheng;[2]WUWeiren;[3]JINGulei;[1]ZHUJun

机构地区：[1]InstituteofBioinformatics,ZhejiangUniversity,Hangzhou310029,China;[2]InstituteofBioinformatics,ZhejiangUniversity,Hangzhou310029,China//CollegeofCropScience,FujianAgricultureandForestryUniversity,Fuzhou350002,China;[3]CollegeofCropScience,FujianAgricultureandForestryUniversity,Fuzhou350002,China

出　　处：《Chinese Science Bulletin》索引2005年第50卷第11期,共6页

基　　金：the National High-Tech Research and Development Program of China(Grant Nos.2003AA207160&2002AA234031); the Natural Science Foundationof Fujian Province(Grant No.B9910011). 摘　　要：By scanning the whole genomic sequence of japonica rice using 45 known plant disease resistance (R) genes, we identified 2119 resistance gene homologs or ana- logs (RGAs) and verified that RGAs are not randomly dis- tributed but tend to cluster in the rice genome. The RGAs were classified into 21 families according to their functional domain based on Hidden Markov model (HMM). By com- paring the RGAs of japonica rice with the whole genomic sequence of indica rice, we found 702 RGAs allelic between the two subspecies and revealed that 671 (95.6%) of them have length difference (InDels) in their genomic sequences (including coding and non-coding regions) between the two subspecies, suggesting that RGAs are highly polymorphic between the two subspecies in rice. We also exploited 402 PCR-based and co-dominant candidate RGA markers by designing primer pairs on the regions flanking the InDels and validating them via e-PCR. The length differences of the candidate RGA markers between the two subspecies are from 1 to 742 bp, with an average of 10.26 bp. All related information of the RGAs is available from our web site (http://gffzzbe43f054107d49d7h9nk99cxku00060vw.ffgz.tsg.suse.edu.cn/RGAs/index.html). 关键词：基因组 R遗传因子水稻 RGA 抵抗力基因

相关文献

参考文献（5）
引证文献（10）
耦合文献（0）

参考文献(5)

引证文献(10)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Genome-wide identification of R genes and exploitation of candidate RGA markers in rice

参考文献(5)

引证文献(10)

用户提示