Molecular cloning of human heat shock protein 27 and study of its protective effects on oxidative damage in rat cardiomyocte H9c2

查看全文 作　　者：Li [1]Liu;Xiaojin [2]Zhang;Surong [1]Jiang;Xiang [1]Gao;Guoxain [1]Ding;Yunlin [1]Cheng

机构地区：[1]Department of Gerontology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;;[2]Center of Model Animal, Nanjing University, Nanjing 210045,China.

出　　处：《Journal of Nanjing Medical University》索引2005年第19卷第4期,共4页

基　　金：ChuangxinFundingofNanjingMedicalUniversity(CX2003018) 摘　　要：Objective: To clone human cardiac heat shock protein 27(HSP27) gene and to determine the effects of HSP27 on the oxidative stress in rat cardiomyocyte cell line H9c2. Methods: Full length of HSP27 cDNA which got by RT-PCR was constructed into pCDNA3.1+. The recombinant was transfected into rat cardiomyocyte cell line H9c2 and the stable transfection cell line was selected by G418. Then we observe the effects of HSP27 over-expression on LDH release and apoptosis induced H2O2 in H9c2. Results:①pCDNA3.1+/HSP27 provided a sound expression of HSP27 in both 293T and H9c2. ②LDH releasing induced by 0, 100,250,500,1000 μmol/L H2O2 in HSP27 over-expression group and wild type group were 0.396±0.017 vs. 0.390±0.009 (p>0.05), 0.437±0.014 vs. 0.416±0.015 (P<0.05), 0.471±0.018 vs. 0.417±0.009 (P<0.001), 0.505±0.030 vs. 0.657±0.022(P<0.001),0.547±0.027 and 0.661±0.011(P<0.001),respectively. ③Apoptosis induced by 150 μmol/L H2O2 in HSP27 over-expression group and wild type group were (10.693±1.122)% vs. (4.027±1.628)%(P<0.01). Conclusion: We cloned and constructed human cardiac HSP27 gene successfully, and over-expression of human HSP27 could inhibit oxidative damage significantly in H9c2. 关键词：分子系化人类热休克蛋白27 保护机制氧化损伤 H9C2 心肌细胞

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Molecular cloning of human heat shock protein 27 and study of its protective effects on oxidative damage in rat cardiomyocte H9c2

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