Fluorescence study on the interactions of PAMAM dendrimers and their derivatives with bovine serum albumin

查看全文 作　　者：WANG Yanming SONG Yu KONG Deling YU [1]Yaoting 机构地区：[1]Bioactive Material Research Laboratory, Nankai University, Tianjin 300071, China 出　　处：《Chinese Science Bulletin》索引2005年第50卷第21期,共6页 基　　金：supported by the National Natural Science Foundation of China(Grant No.30370405). 摘　　要：The interactions of amino-terminated, and ethylenediamine core poly(amidoamine) (PAMAM) den-drimers and their derivatives with bovine serum albumin (BSA) were investigated by fluorescence spectroscopy. Ex-perimental results showed that the fluorescence intensity of BSA decreased after the addition of different modified den-drimers, and the extent of the fluorescence quenching caused by various modified dendrimers strongly depends upon the different functional groups on their surfaces. We also inves-tigated the influence of pH and ionic strength on the interac-tion between various modified dendrimers and BSA. Circu-lar dichroism (CD) spectroscopic measurements showed that the content of α-helix structure of BSA decreased after the addition of different modified dendrimers, which indicated that dendrimers induced changes in the secondary structure of BSA. 关 键 词：PAMAM 高分子化合物 化学结构 荧光性 分色性