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Capsaicin-induced cell death in a human gastric adenocarcinoma cell line

查看全文 作　　者：Yi-Ching [1]Lo;Yuan-Chieh [2]Yang;I-Chen [3]Wu;Fu-Chen [4]Kuo;Chi-Ming [1]Liu;Hao-Wei [1]Wang;Chao-Hung [3]Kuo;Jeng-Yi [3]Wu;Deng-Chyang [3]Wu 高影响力作者 机构地区：[1]Department and Graduate Institute of Pharmacology, Kaohsiung Medical University,Kaohsiung City, Taiwan, China;[2]Department of Clinical Pathology, KaohsiungMedical University Hospital, Kaohsiung City, Taiwan, China;[3]Division of Gastroenterology, Department of Internal Medicine,Kaohsiung Medical University Hospital. Kaohsiung City, Taiwan,China;[4]Department of Gynecology and Obstetrics, E-DAHospital, I-Shou University, Kaohsiung, Taiwan, China高影响力机构 出　　处：《World Journal of Gastroenterology》索引2005年第11卷第40期,共4页高影响力期刊 基　　金：Supported by Grants from the National Science Council of the ROC, No. NSC 89-2314-B-037-073 and NSC-89-2315-B-037-004 摘　　要：AIM: Capsaicin, a pungent ingredient found in red pepper,has long been used in spices, food additives, and drugs.Cell death induced by the binding of capsaicin was examined in a human gastric adenocarcinoma cell line (AGS cells).METHODS: By using XTT-based cytotoxicityassay, flow cytometry using the TUNEL method, and quantitation of DNA fragmentation, both cell death and DNA fragmentation were detected in AGS cells treated with capsaicin. By using Western blotting methods, capsaicin reduced the expression of Bcl-2, the antiapoptotic protein, in AGS cells in a concentration-dependent manner.RESULTS: After incubation of AGS cells with capsaicin for 24 h, cell viability decreased significantly in a dose-dependent manner. After incubation of AGS cells with capsaicin for 24 h, apoptotic bodies also significantly increased, and were again correlated with the dose of capsaicin. When the concentration of capsaicin was 1 mmol/L, the amount of DNA fragments also increased. Similar results werealso in the lower traces.CONCLUSION: These results suggest that capsaicininduced cell death might be via a Bcl-2 sensitive apoptotic pathway. Therefore, capsaicin might induce protection from gastric cancer. 关 键 词：辣椒素 细胞凋亡 胃癌 肿瘤细胞
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