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RP-HPLC Determination and Pharmacokinetic Comparison of Cinnamic Acid in Rat Plasma After Administration of Di-Gu-Pi Decoction and Pure Cinnamic Acid

查看全文 作　　者：LI [1]Kang;BI Kai-[2]shun 高影响力作者 机构地区：[1]Department of Pharmacy, Shenzhen Hospital, Beijing University, Shenzhen 518036, P.R. China;[2]College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, P.R. China高影响力机构 出　　处：《Chemical Research in Chinese Universities》索引2006年第22卷第1期,共5页高影响力期刊 摘　　要：A sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was extracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase(HPLC) system with a Diamonsil C_ 18 column and methanol-acetonitrile-water (8∶32∶60, volume ratio) (adjusted to pH=3.0 with glacial acetic acid) as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-(p-fluorophenyl)-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0.10 to 25.0 μg/mL (R 2 =0.9988, n=9). The precision was 3.42%—10.10%; the between-day precision was 2.84%—8.91%; the accuracy was-1.51%—1.26%; the mean recovery was 99.9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid. 关 键 词：Di-Gu-Pi煎剂 肉桂酸 药物代谢动力学 RP-HPLC
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